Strengths and weaknesses of in-tube solid-phase microextraction: A scoping review

Fernández-Amado, María; Prieto-Blanco, M.C.; López-Mahı́a, Purificación; Muniategui-Lorenzo, Soledad; Prada‐Rodríguez, D.

doi:10.1016/j.aca.2015.12.007

Cited by 101 publications

(41 citation statements)

References 91 publications

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“…For simultaneous quantitative analysis of multi-class plant hormones, the compatibility of an in vivo and automated sample pretreatment methods with dynamic and ultrasensitive extraction capacity are required. SPME has advantages in these aspects [48,50,52,79]. In SPME, sampling, extraction, concentration, and injection are integrated into a single step, which promotes miniaturization, automation, in vivo sampling and on-line analysis [80].…”

Section: Spme Strategies and Advancesmentioning

confidence: 99%

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

et al. 2020

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Plant hormones are naturally occurring small molecule compounds which are present at trace amounts in plant. They play a pivotal role in the regulation of plant growth. The biological activity of plant hormones depends on their concentrations in the plant, thus, accurate determination of plant hormone is paramount. However, the complex plant matrix, wide polarity range and low concentration of plant hormones are the main hindrances to effective analyses of plant hormone even when state-of-the-art analytical techniques are employed. These factors substantially influence the accuracy of analytical results. So far, significant progress has been realized in the analysis of plant hormones, particularly in sample pretreatment techniques and mass spectrometric methods. This review describes the classic extraction and modern microextraction techniques used to analyze plant hormone. Advancements in solid phase microextraction (SPME) methods have been driven by the ever-increasing requirement for dynamic and in vivo identification of the spatial distribution of plant hormones in real-life plant samples, which would contribute greatly to the burgeoning field of plant hormone investigation. In this review, we describe advances in various aspects of mass spectrometry methods. Many fragmentation patterns are analyzed to provide the theoretical basis for the establishment of a mass spectral database for the analysis of plant hormones. We hope to provide a technical guide for further discovery of new plant hormones. More than 140 research studies on plant hormone published in the past decade are reviewed, with a particular emphasis on the recent advances in mass spectrometry and sample pretreatment techniques in the analysis of plant hormone. The potential progress for further research in plant hormones analysis is also highlighted.

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Section: Spme Strategies and Advancesmentioning

confidence: 99%

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

et al. 2020

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“…Even though in-tube SPME was originally conceived for LC applications, it can also be on-line coupled directly to UV-vis [63], and direct analysis in real time (DART-MS) [64], or off-line with MALDI-TOF [65,66] detectors. Fernández-Amado et al [67] recently provided a comprehensive overview of the strengths and weaknesses of in-tube SPME, showing the possible different couplings and configurations, and new developments made within this technique (Figure 3). In-tube SPME can easily be automated and carried out in two extraction modes: Flow-through or draw/eject cycles (Figure 4).…”

Section: Extraction Modes and Operational Devicesmentioning

confidence: 99%

Current Trends in Fully Automated On-Line Analytical Techniques for Beverage Analysis

2019

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The determination of target analytes in complex matrices such as beverages requires a series of analytical steps to obtain a reliable analysis. This critical review presents the current trends in sample preparation techniques based on solid phase extraction miniaturization, automation and on-line coupling. Techniques discussed include solid-phase extraction (SPE), solid-phase microextraction (SPME), in-tube solid-phase microextraction (in-tube SPME) and turbulent-flow chromatography (TFC). Advantages and limitations, as well as several of their main applications in beverage samples are discussed. Finally, fully automated on-line systems that involve extraction, chromatographic separation, and tandem mass spectrometry in one-step are introduced and critically reviewed.

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“…A subsequent change in the valve position allows the desorption and transfer of the retained analytes to the separative column by means of the mobile-phase. Today, IT-SPME is a well-established technique that has been successfully used in many fields of applications [1][2][3]. This technique is especially well-suited for miniaturized LC systems such as Capillary-LC (Cap-LC) and Nano-LC.…”

Section: Introductionmentioning

confidence: 99%

Exploring New Extractive Phases for In-Tube Solid Phase Microextraction Coupled to Miniaturized Liquid Chromatography

et al. 2019

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In-tube solid-phase microextraction (IT-SPME) coupled on-line to miniaturized liquid chromatography (LC) has emerged as a powerful tool to address a variety of analytical problems. However, in order to expand its applicability, the development of new sorbents that enhance the efficiency and specificity of the extraction is highly desirable. In this respect, the employment of capillary columns coated with sorbents functionalized with nanoparticles (NPs) replacing the loop of the injection valve (in-valve IT-SPME) is one of the most attractive options. In this work, polymers of tetraethyl orthosilicate (TEOS) and trimethoxyethylsilane (MTEOS) modified with SiO2 and TiO2 NPs have been synthetized and used for the extraction of a variety of water pollutants, using both Capillary-LC and Nano-LC. Compounds with different chemical structures and polarities such as the artificial sweetener saccharine, the polycyclic aromatic hydrocarbons (PAHs) naphthalene and fluoranthene, and some phenylurea and organophosphorous herbicides have been used as target analytes. The extraction efficiencies found with the synthetized capillaries have been compared to those obtained with commercially available capillaries coated with polydiphenyl-polydimethylsiloxane (PDMS), nitroterephthalic acid modified polyetilenglicol (FFAP), and polystyrene-divinylbenzene (PS-DVB) phases. The results obtained in this preliminary study showed that, although PS-DVB phase has the strongest affinity for compounds with two or more aromatic rings, the extraction with TEOS-MTEOS coatings modified with NPs is the best option for a majority of the tested compounds. Examples of application are given.

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Strengths and weaknesses of in-tube solid-phase microextraction: A scoping review

Cited by 101 publications

References 91 publications

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

Current Trends in Fully Automated On-Line Analytical Techniques for Beverage Analysis

Exploring New Extractive Phases for In-Tube Solid Phase Microextraction Coupled to Miniaturized Liquid Chromatography

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