Strengthening of lateral activation in adult rat visual cortex after retinal lesions captured with voltage-sensitive dye imaging in vivo

Palagina, Ganna; Eysel, Ulf T.; Jancke, Dirk

doi:10.1073/pnas.0900068106

Cited by 43 publications

(37 citation statements)

References 41 publications

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“…In the future, these capacities might provide a powerful approach to study cortical reorganization processes (70,71) of the functional neuronal architecture induced by TMSbased plasticity and learning protocols. Heart rate, intratracheal pressure, exhaled CO 2 , and body temperature were monitored.…”

Section: Discussionmentioning

confidence: 99%

Voltage-sensitive dye imaging of transcranial magnetic stimulation-induced intracortical dynamics

Kozyrev

Eysel

Jancke

2014

Proc. Natl. Acad. Sci. U.S.A.

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Transcranial magnetic stimulation (TMS) is widely used in clinical interventions and basic neuroscience. Additionally, it has become a powerful tool to drive plastic changes in neuronal networks. However, highly resolved recordings of the immediate TMS effects have remained scarce, because existing recording techniques are limited in spatial or temporal resolution or are interfered with by the strong TMS-induced electric field. To circumvent these constraints, we performed optical imaging with voltage-sensitive dye (VSD) in an animal experimental setting using anaesthetized cats. The dye signals reflect gradual changes in the cells' membrane potential across several square millimeters of cortical tissue, thus enabling direct visualization of TMS-induced neuronal population dynamics. After application of a single TMS pulse across visual cortex, brief focal activation was immediately followed by synchronous suppression of a large pool of neurons. With consecutive magnetic pulses (10 Hz), widespread activity within this "basin of suppression" increased stepwise to suprathreshold levels and spontaneous activity was enhanced. Visual stimulation after repetitive TMS revealed long-term potentiation of evoked activity. Furthermore, loss of the "deceleration-acceleration" notch during the rising phase of the response, as a signature of fast intracortical inhibition detectable with VSD imaging, indicated weakened inhibition as an important driving force of increasing cortical excitability. In summary, our data show that high-frequency TMS changes the balance between excitation and inhibition in favor of an excitatory cortical state. VSD imaging may thus be a promising technique to trace TMS-induced changes in excitability and resulting plastic processes across cortical maps with high spatial and temporal resolutions. (1) (TMS) has become a frequently used method for noninvasive diagnostics, therapeutic treatment, and intervention for neurorehabilitation of neurological disorders (2-8). Additionally, TMS has proved a valuable tool in basic brain research as its perturbative effects allow area-selective manipulation of immediate cortical function (9-11), as well as its long-lasting alteration through plasticity and learning protocols (12, 13). However, direct measurements of the TMS-induced cortical dynamics at highly resolved spatiotemporal scales are missing because "online approaches" (14), using modern neuroimaging techniques such as functional MRI (fMRI) (15-18), magnetoencephalography (19), EEG (20), and near-infrared (21) or intrinsic optical imaging (22), are limited in either spatial or temporal resolutions or in both.Here we overcame these limitations, using optical imaging with voltage-sensitive dyes (VSD), which exploits the dye's property to transduce gradual changes in voltage across neuronal membranes into fluorescent light signals. In contrast to imaging methods applicable in humans, this method is invasive but allows avoiding the commonly experienced contamination of signals by artifacts due to the strong ...

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Section: Discussionmentioning

confidence: 99%

Voltage-sensitive dye imaging of transcranial magnetic stimulation-induced intracortical dynamics

Kozyrev

Eysel

Jancke

2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Secondly, there is an extensive remapping and reorganization of the visual field representation after pre-and post-chiasmatic damage as shown in animal model studies [34][35][36][37], as well as behavioral [38] and fMRI studies in patients [31][32][33]39]. Such receptive field reorganization is believed to be mediated by mechanisms involving plasticity of intrinsic cortical horizontal connections [40]. Importantly, receptive fields modifications are found not only within the damaged tissue or lesion projection zone, but they also occur in the area adjacent to the scotoma but not directly affected by the lesion [35,37].…”

Section: Perceptual Deficits In the ''Intact'' Visual Field Of Patientsmentioning

confidence: 99%

The second face of blindness: Processing speed deficits in the intact visual field after pre- and post-chiasmatic lesions

Bola

Gall

Sabel

2013

Journal of the Neurological Sciences

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Purpose: Damage along the visual pathway results in a visual field defect (scotoma), which retinotopically corresponds to the damaged neural tissue. Other parts of the visual field, processed by the uninjured tissue, are considered to be intact. However, perceptual deficits have been observed in the ''intact'' visual field, but these functional impairments are poorly understood. We now studied temporal processing deficits in the intact visual field of patients with either pre-or postchiasmatic lesions to better understand the functional consequences of partial blindness.Methods: Patients with pre-(n = 53) or post-chiasmatic lesions (n = 98) were tested with high resolution perimetry -a method used to map visual fields with supra-threshold light stimuli. Reaction time of detections in the intact visual field was then analyzed as an indicator of processing speed and correlated with features of the visual field defect.

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“…Optical imaging of VSD was so far mostly applied to explore the in vivo functional architecture of the mammalian neocortex at high spatial (up to 50 m) and temporal (millisecond) resolution (Grinvald et al, 1994;Seidemann et al, 2002;Jancke et al, 2004;Sharon et al, 2007;Palagina et al, 2009) (but see Prechtl et al, 1997;Senseman and Robbins, 1999, for VSD imaging in the turtle visual brain in vivo). The VSD binds to the external surfaces of all excitable membranes.…”

Section: Vsd Imagingmentioning

confidence: 99%

Dominant Vertical Orientation Processing without Clustered Maps: Early Visual Brain Dynamics Imaged with Voltage-Sensitive Dye in the Pigeon Visual Wulst

Ng¹,

Grabska-Barwińska²,

Güntürkün³

et al. 2010

J. Neurosci.

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The pigeon is a widely established behavioral model of visual cognition, but the processes along its most basic visual pathways remain mostly unexplored. Here, we report the neuronal population dynamics of the visual Wulst, an assumed homolog of the mammalian striate cortex, captured for the first time with voltage-sensitive dye imaging. Responses to drifting gratings were characterized by focal emergence of activity that spread extensively across the entire Wulst, followed by rapid adaptation that was most effective in the surround. Using additional electrophysiological recordings, we found cells that prefer a variety of orientations. However, analysis of the imaged spatiotemporal activation patterns revealed no clustered orientation map-like arrangements as typically found in the primary visual cortices of many mammalian species. Instead, the vertical orientation was overrepresented, both in terms of the imaged population signal, as well as the number of neurons preferring the vertical orientation. Such enhanced selectivity for the vertical orientation may result from horizontal motion vectors that trigger adaptation to the extensive flow field input during natural behavior. Our findings suggest that, although the avian visual Wulst is homologous to the primary visual cortex in terms of its gross anatomical connectivity and topology, its detailed operation and internal organization is still shaped according to specific input characteristics.

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Strengthening of lateral activation in adult rat visual cortex after retinal lesions captured with voltage-sensitive dye imaging in vivo

Cited by 43 publications

References 41 publications

Voltage-sensitive dye imaging of transcranial magnetic stimulation-induced intracortical dynamics

Voltage-sensitive dye imaging of transcranial magnetic stimulation-induced intracortical dynamics

The second face of blindness: Processing speed deficits in the intact visual field after pre- and post-chiasmatic lesions

Dominant Vertical Orientation Processing without Clustered Maps: Early Visual Brain Dynamics Imaged with Voltage-Sensitive Dye in the Pigeon Visual Wulst

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