Streamlined F2-Isoprostane Analysis in Plasma and Urine with High-Performance Liquid Chromatography and Gas Chromatography/Mass Spectroscopy

Walter, Mary; Blumberg, Jeffrey B.; Dolnikowski, Gregory G.; Handelman, Garry J.

doi:10.1006/abio.1999.4476

Cited by 66 publications

(43 citation statements)

References 19 publications

(19 reference statements)

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“…An advantage of measuring urinary isoprostanes is that neither 15-F 2␣ -isoprostanes (reflecting systemic rather than renal formation) nor 5-F 2␣ -isoprostane are formed ex vivo by autooxidation in urine. Isoprostanes were measured in duplicate by gas chromatography/mass spectrometry after isolation using high-pressure liquid chromatography according to the method described by Sachek et al (2003) and modified from that of Walter et al (2000). Briefly, samples were thawed, and deuterated prostaglandin F 2␣ was added as an internal standard.…”

Section: Methodsmentioning

confidence: 99%

Superoxide Dismutase Mimetic Preserves the Glomerular Capillary Permeability Barrier to Protein

Duann

Datta

Pan

et al. 2005

J Pharmacol Exp Ther

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Overproduction of superoxide (O 2 . ) occurs in glomerular disease and may overwhelm the capacity of superoxide dismutase (SOD), thereby intensifying oxidant injury by O 2 . and related radical species that disrupt the glomerular capillary permeability barrier to protein. We examined the efficacy of the SOD mimetic tempol in preserving glomerular permeability to protein using 1) a rat model of glomerular immune injury induced by an antiglomerular basement membrane antibody (anti-GBM), and 2) isolated rat glomeruli in which injury was induced by the cytokine tumor necrosis factor-␣ (TNF␣). To induce glomerular immune injury, rats received anti-GBM using a protocol that results in prominent infiltration of glomeruli by macrophages and in which macrophage-derived TNF␣ has been shown to mediate albuminuria. To increase glomerular capillary permeability to albumin (P alb ) ex vivo, isolated glomeruli were incubated with TNF␣ at concentrations (0.5-4.0 g/ml) known to stimulate O 2 . production. Increments in P alb were detected by measuring changes in glomerular volume in response to an applied oncotic gradient. Significant increases in the urine excretion of albumin and F 2␣ -isoprostane were observed in rats with glomerular immune injury without a significant change in systolic blood pressure. Tempol treatment significantly reduced urine isoprostane and albumin excretion. In isolated glomeruli, TNF␣ increased P alb and tempol abrogated this effect, both in a dose-dependent manner. These observations indicate that SOD mimetics can preserve the glomerular permeability barrier to protein under conditions of oxidative stress from O 2 . production.

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Section: Methodsmentioning

confidence: 99%

Superoxide Dismutase Mimetic Preserves the Glomerular Capillary Permeability Barrier to Protein

Duann

Datta

Pan

et al. 2005

J Pharmacol Exp Ther

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“…F 2α -isoprostanes in urine were measured using an HPLC and GC/MS method as described by Walter et al [32]. The final values were adjusted with urinary creatinine to account for differences in urine volume.…”

Section: Determination Of Biochemical Biomarkersmentioning

confidence: 99%

Chronic consumption of a low calorie, high polyphenol cranberry beverage attenuates inflammation and improves glucoregulation and HDL cholesterol in healthy overweight humans: a randomized controlled trial

et al. 2018

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Purpose We studied the health benefits of low calorie cranberry beverage consumption on glucoregulation, oxidative damage, inflammation, and lipid metabolism in overweight but otherwise healthy humans. Methods 78 overweight or obese men and women (30-70 years; BMI 27-35 kg/m 2 ) with abdominal adiposity (waist: hip > 0.8 for women and > 0.9 for men; waist: height ≥ 0.5) consumed 450 mL placebo or low calorie, high polyphenol cranberry extract beverage (CEB) daily for 8 week in a randomized, double-blind, placebo-controlled, parallel design trial. Blood and urine samples were collected after overnight fast at baseline and after 8 weeks of daily beverage consumption. Blood and urine samples were also collected during 3 oral glucose tolerance test (OGTT) challenges: (1) pre-intervention without the test beverages, (2) following a single dose of placebo or CEB at baseline (week 0), and (3) following a single dose of placebo or CEB at 8 week. Results Compared to placebo, a single CEB dose at baseline lowered endothelin-1 and elevated nitric oxide and the reduced:oxidized glutathione ratio (P < 0.05). Interferon-γ was elevated (P < 0.05) after a single CEB dose at baseline; however, after 8 week of CEB intervention, fasting C-reactive protein was lower (P < 0.05). CEB consumption for 8 week also reduced serum insulin and increased HDL cholesterol compared to placebo (P < 0.05). Conclusions An acute dose of low calorie, high polyphenol cranberry beverage improved antioxidant status, while 8 week daily consumption reduced cardiovascular disease risk factors by improving glucoregulation, downregulating inflammatory biomarkers, and increasing HDL cholesterol.

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“…Total levels of F 2 -isoprostanes were measured in MLVs reconstituted from DAPC prepared in the presence of vehicle or various statins and Trolox (100 nM), using GC-MS with negative chemical ionization as described by Walter et al (23). MLVs were allowed to autoxidize for 48 h at 37°C, and peroxidation was terminated by the addition of 25 l of 5.0 mM EDTA and 20 l of 35.0 mM butylated hydroxytoluene.…”

Section: Preparation Of Multilamellar Lipid Vesicles (Mlvs) For Lipidmentioning

confidence: 99%

Active Metabolite of Atorvastatin Inhibits Membrane Cholesterol Domain Formation by an Antioxidant Mechanism

Mason

Walter²,

Day³

et al. 2006

Journal of Biological Chemistry

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The advanced atherosclerotic lesion is characterized by the formation of microscopic cholesterol crystals that contribute to mechanisms of inflammation and apoptotic cell death. These crystals develop from membrane cholesterol domains, a process that is accelerated under conditions of hyperlipidemia and oxidative stress. In this study, the comparative effects of hydroxymethylglutaryl-CoA (HMG-CoA) reductase inhibitors (statins) on oxidative stress-induced cholesterol domain formation were tested in model membranes containing physiologic levels of cholesterol using small angle x-ray diffraction approaches. In the absence of HMG-CoA reductase, only the atorvastatin active o-hydroxy metabolite (ATM) blocked membrane cholesterol domain formation as a function of oxidative stress. This effect of ATM is attributed to electron donation and proton stabilization mechanisms associated with its phenoxy group located in the membrane hydrocarbon core. ATM inhibited lipid peroxidation in human low density lipoprotein and phospholipid vesicles in a dose-dependent manner, unlike its parent and other statins (pravastatin, rosuvastatin, simvastatin). These findings indicate an atheroprotective effect of ATM on membrane lipid organization through a potent antioxidant mechanism.The unstable atherosclerotic lesion is characterized by extracellular lipid deposits consisting of cholesterol (both free and esterified), phospholipids, and lesser amounts of triacylglycerol (1). Free cholesterol is associated with phospholipid membranes and insoluble, extracellular crystals in the lipid core of the plaque. Membrane-associated cholesterol crystals have been characterized in cell culture systems and tissue explants from animal models of atherosclerosis using electron microscopy and x-ray diffraction approaches (2, 3). Microscopic cholesterol crystalline structures have also been observed in macrophage foam cells following treatment with LDL 2 or by inhibition of acyl-coenzyme A:cholesterol acyltransferase (4 -6). These crystalline structures contribute to mechanisms of cell death and inflammation (3-5). Although non-crystalline membrane cholesterol can readily exchange from the plaque with plasma lipoprotein particles, cholesterol in the crystalline state is insoluble and does not respond to pharmacologic intervention or reverse cholesterol transport mechanisms (1).We have recently reported that oxidative stress, a pathologic process associated with cardiovascular risk factors (e.g. hypertension, diabetes, hypercholesterolemia) (7), contributes directly to the formation of cholesterol crystalline microdomains in membranes (8). Domain formation as a function of lipid peroxidation was observed in lipid vesicles containing physiologic levels of cholesterol using small angle x-ray diffraction approaches (2, 9). These observations have led to our current hypothesis that the active o-hydroxy metabolite of atorvastatin (ATM) may interfere with cholesterol domain formation without altering membrane cholesterol content. ATM was selected as it has pot...

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Streamlined F2-Isoprostane Analysis in Plasma and Urine with High-Performance Liquid Chromatography and Gas Chromatography/Mass Spectroscopy

Cited by 66 publications

References 19 publications

Superoxide Dismutase Mimetic Preserves the Glomerular Capillary Permeability Barrier to Protein

Superoxide Dismutase Mimetic Preserves the Glomerular Capillary Permeability Barrier to Protein

Chronic consumption of a low calorie, high polyphenol cranberry beverage attenuates inflammation and improves glucoregulation and HDL cholesterol in healthy overweight humans: a randomized controlled trial

Active Metabolite of Atorvastatin Inhibits Membrane Cholesterol Domain Formation by an Antioxidant Mechanism

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