Strawberry Parent Clones US 70, US 159, US 292, and US 438 Resistant to Anthracnose Crown Rot

Galletta, Gene J.; Smith, Barbara J.; Gupton, C. L.

doi:10.21273/hortsci.28.10.1055

Cited by 19 publications

(14 citation statements)

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“…DNAs from the Fragaria · ananassa cultivars Camarosa, Sweet Charlie, Pa´jaro, Oso Grande, Gaviota and Milsei tudla, the breeding line US159 (Galletta et al 1993), one accession of the related wild species Fragaria vesca and F. chiloensis, and two different accessions of Duchesnea indica, with different responses to the anthracnose disease (Colletotrichum spp. ), were used for RGA isolation.…”

Section: Plant Materials and Dna Extractionmentioning

confidence: 99%

Isolation and diversity analysis of resistance gene analogues (RGAs) from cultivated and wild strawberries

2004

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Degenerate oligonucleotide primers, designed based on conserved regions of Nucleotide Binding Site (NBS) domains from previously cloned plant resistance genes, were used to isolate Resistance Gene Analogues (RGAs) from wild and cultivated strawberries. Seven distinct families of RGAs of the NBS-LRR type were identified from two related wild species, Fragaria vesca and F. chiloensis, and six different Fragaria x ananassa cultivars. With one exception (GAV-3), the deduced amino acid sequences of strawberry RGAs showed strong similarity to TIR (Toll Interleukin I Receptor)-type R genes from Arabidopsis, tobacco and flax, suggesting the existence of common ancestors. GAV-3 seemed to be more closely related to the non-TIR type. Further studies showed that the recombination level and the ratio of non-synonymous to synonymous substitutions within families were low. These data suggest that NBS-encoding sequences of RGAs in strawberry are subject to a gradual accumulation of mutations leading to purifying selection, rather than to a diversifying process. The present paper is the first report on RGAs in strawberry.

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Section: Plant Materials and Dna Extractionmentioning

confidence: 99%

Isolation and diversity analysis of resistance gene analogues (RGAs) from cultivated and wild strawberries

2004

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“…This scale is based on percent disease determined by image analysis: DSR = 0 is equivalent to 0 to 0.7% disease, DSR = 1 is equivalent to >0.7 to 3.5% disease, DSR = 2 is equivalent to >3.5% to 10% disease, DSR = 3 is equivalent to >10% to 35% disease, DSR = 4 is equivalent to >35% to 70% disease, and DSR = 5 is equivalent to >70% disease. Prior research (Miller-Butler et al, 2018) indicated that detached leaf DSRs #0.8 indicate a resistant response based on a strong correlation between the detached leaf assay and the whole plant assay which has been used for more than 30 years to identify strawberry plants with resistance to anthracnose crown rot caused by C. fragariae and C. gloeosporioides (Galletta et al, 1993;Lewers et al, 2004Lewers et al, , 2017Smith et al, 1998). When rating leaves in the detached leaf assay, we suggest that a DSR of #0.8 based on the visual scale shown in Fig.…”

Section: Discussionmentioning

confidence: 85%

Evaluation of Detached Strawberry Leaves for Anthracnose Disease Severity Using Image Analysis and Visual Ratings

Miller-Butler¹,

Smith²,

Curry³

et al. 2019

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Inoculation of detached strawberry leaves with Colletotrichum species may provide an accurate, rapid, nondestructive method of identifying anthracnose-resistant germplasm. The purpose of this study was to statistically compare two methods (visual and image analysis) of evaluating disease severity of strawberry germplasm screened for anthracnose resistance. Detached leaves of 77 susceptible and resistant strawberry clones were inoculated with one Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. and two C. fragariae A. N. Brooks isolates. Anthracnose disease symptoms on each leaf were assessed quantitatively via computer-based image analysis to determine percentage lesion area and qualitatively by two independent raters using a visual disease severity rating scale (0 = no symptoms to 5 = entire leaf dead). The two visual raters’ average disease severity ratings (n = 3413) were in substantial agreement with a weighted Cohen’s kappa coefficient (k) of 0.80 [95% confidence interval (CI) 0.79–0.82]. There was a strong positive correlation between percent lesion area determined by image analysis and the visual disease scores of the two raters (rp = 0.79). Image analysis provided a precise measurement of percent lesion area of infected leaves while visual assessment provided more rapid results. Our results indicate that detached leaf inoculations can be used as a rapid preliminary screen to separate anthracnose-susceptible from -resistant germplasm in large populations within breeding programs. It also may be used for assessing the resistance/susceptibility of parental breeding lines to various Colletotrichum species and isolates, for mapping germplasm for resistance genes, and in pesticide development studies.

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“…The USDA-ARS program resulted in over 1500 anthracnose resistant selections that were made from the seedlings field-tested in Mississippi. Four of these anthracnose-resistant strawberry clones were released as breeding lines (Galletta et al, 1993) and one was released as the cultivar Pelican, which is highly resistant to anthracnose crown rot caused by C. fragariae and to anthracnose fruit rot caused by C. acutatum (Smith et al, 1998). 'Pelican' is also resistant to five races of red stele caused by Phytophthora fragariae.…”

Section: Strawberry Breeding Programs To Develop Anthracnose Resistanmentioning

confidence: 99%

Strawberry Anthracnose: Progress toward Control through Science

Smith

2013

International Journal of Fruit Science

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Strawberry Parent Clones US 70, US 159, US 292, and US 438 Resistant to Anthracnose Crown Rot

Cited by 19 publications

References 0 publications

Isolation and diversity analysis of resistance gene analogues (RGAs) from cultivated and wild strawberries

Isolation and diversity analysis of resistance gene analogues (RGAs) from cultivated and wild strawberries

Evaluation of Detached Strawberry Leaves for Anthracnose Disease Severity Using Image Analysis and Visual Ratings

Strawberry Anthracnose: Progress toward Control through Science

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