Stratum corneum lipids liposomes for the topical delivery of 5-aminolevulinic acid in photodynamic therapy of skin cancer: preparation and in vitro permeation study

Abstract

BackgroundPhotodynamic therapy (PDT) using 5-aminolevulinic acid (5-ALA) is a skin cancer therapy that still has limitations due to the low penetration of this drug into the skin. We have proposed in this work a delivery system for 5-ALA based on liposomes having lipid composition similar to the mammalian stratum corneum (SCLLs) in order to optimize its skin delivery in Photodynamic Therapy (PDT) of skin cancers.MethodsSCLLs were obtained by reverse phase evaporation technique and size distribution of the vesicles was determinated by photon correlation spectroscopy. In vitro permeation profile was characterized using hairless mouse skin mounted in modified Franz diffusion cell.ResultsSize exclusion chromatography on gel filtration confirmed vesicle formation. SCLLs obtained by presented a degree of encapsulation of 5-ALA around 5.7%. A distribution of vesicle size centering at around 500 nm and 400 nm respectively for SCLLs and SCLLs containing 5-ALA was found. In vitro 5-ALA permeation study showed that SCLLs preparations presented higher skin retention significantly (p < 0.05) on the epidermis without SC + dermis, with a decreasing of skin permeation compared to aqueous solution.ConclusionsThe in vitro delivery performance provided by SCLLs lead to consider this systems adequate for the 5-ALA-PDT of skin cancer, since SCLLs have delivered 5-ALA to the target skin layers (viable epidermis + dermis) to be treated by topical PDT of skin cancer.

Synergistic effects of 5-aminolevulinic acid based photodynamic therapy and celecoxib via oxidative stress in human cholangiocarcinoma cells

“…Accumulation of ROS in substantial concentrations damages cellular DNA and/or membranes, and induces cell death. [9][10][11][12][13][14] We have previously reported that ALA-PDT could effectively inhibit HuCC-T1 cell survival via increased production of intracellular ROS. 19 To further improve the effect of ALA-PDT on HuCC-T1 cells, we sought to boost ROS levels after PDT.…”

“…[3][4][5][6]9,10 Excitation of a photosensitizer by irradiation with a suitable light source can produce a significant amount of reactive oxygen species (ROS). 9,10 ROS elicit DNA and cell membrane damage through lipid peroxidation/alterations in membrane fluidity. The damage to cellular organelles, such as mitochondria and microsomes, can be harnessed to increase the death of tumor cells.…”

Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells

“…This fact reduces or avoids the risk of prolonged phototoxicity to the human body. [14][15][16][17] Recently, researchers reported the effects of ALA-based PDT in various tumor cells such as oral squamous cell carcinoma, melanoma, glioma, lymphoma, esophageal carcinoma, and basal cell carcinoma.…”

Desenvolvimento, caracterização e avaliação de sistemas microestruturados para veiculação de ácido retinóico na pele

“…Essa barreira é formada pelos corneócitos que são células inativas formadas no estágio final de diferenciação das células da epiderme . Desta forma, esta camada não é o alvo para fármacos tópicos que têm ação em receptores localizados na epiderme viável e derme, como é o caso do ácido retinóico (PIERRE et al, 2001;). …”