Stratified-structural hydrogel incorporated with magnesium-ion-modified black phosphorus nanosheets for promoting neuro-vascularized bone regeneration

Xu, Yan; Xu, Chao; He, Lei; Zhou, Junjie; Chen, Tianwu; Ouyang, Liuzhang; Guo, Xiaodong; Qu, Yanzhen; Luo, Zhiqiang; Duan, Deyu

doi:10.1016/j.bioactmat.2022.02.024

Cited by 71 publications

(88 citation statements)

References 39 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The vascularization of the implanted bone substitute is indispensable for appropriate bone healing since vessels provide the main source of nutrients for the entire bone niche. The human umbilical vein endothelial (HUVECs) cell line is commonly used to assess the effects of bone biomaterial on vessel formation in vitro [ 75 , 105 , 139 , 140 , 142 , 143 , 144 , 145 ]. As an alternative, the use of human aortic endothelial cells (HAECs) is also reported [ 146 ].…”

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

“…As an alternative, the use of human aortic endothelial cells (HAECs) is also reported [ 146 ]. The tube formation assay is a classic angiogenesis assay that can be used to assess the angiogenic differentiation of HUVECs in contact with a specific biomaterial; the tube structures as well as the total branch length can be analyzed with a contrast inverted microscope [ 86 , 105 , 140 , 142 , 143 ].…”

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

“…The expression of angiogenic related genes, such as platelet and endothelial cell adhesion molecule 1 (PECAM1/CD31), vascular endothelial growth factor A (VEGFA), fibroblast growth factor 2 (FGF2), kinase insert domain receptor/vascular endothelial growth factor receptor 2 (KDR), C-X-C motif chemokine ligand 12 (CXCL12/SDF1A), nitric oxide synthase 3 (NOS3 ), and hypoxia inducible factor 1 subunit alpha (HIF1A) can be analyzed by RT-qPCR and/or immunofluorescence and/or Western blot analysis and/or ELISA [ 75 , 86 , 105 , 107 , 139 , 140 , 142 , 143 , 144 , 145 ].…”

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

“…A migration test of endothelial cells by scratch wound healing assay on the biomaterial can be added to the set of tests aimed at assessing the angiogenic properties of a bone-substitute material [ 86 , 105 , 139 , 142 , 143 , 145 ].…”

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

“…Moreover, in this case, the reciprocal effects of precursor bone cells as well as of immune cells on angiogenesis and vice versa in the presence of the tested biomaterials can be determined by culturing different cell types simultaneously [ 75 , 105 , 142 ].…”

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

See 4 more Smart Citations

Translating Material Science into Bone Regenerative Medicine Applications: State-of-The Art Methods and Protocols

Pietro

Palmieri

Papi

et al. 2022

IJMS

View full text Add to dashboard Cite

In the last 20 years, bone regenerative research has experienced exponential growth thanks to the discovery of new nanomaterials and improved manufacturing technologies that have emerged in the biomedical field. This revolution demands standardization of methods employed for biomaterials characterization in order to achieve comparable, interoperable, and reproducible results. The exploited methods for characterization span from biophysics and biochemical techniques, including microscopy and spectroscopy, functional assays for biological properties, and molecular profiling. This review aims to provide scholars with a rapid handbook collecting multidisciplinary methods for bone substitute R&D and validation, getting sources from an up-to-date and comprehensive examination of the scientific landscape.

show abstract

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

See 3 more Smart Citations

Translating Material Science into Bone Regenerative Medicine Applications: State-of-The Art Methods and Protocols

Pietro

Palmieri

Papi

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

Bioprinted Scaffold Remodels the Neuromodulatory Microenvironment for Enhancing Bone Regeneration

Guo

2023

Adv Funct Materials

View full text Add to dashboard Cite

Herein, a 3D bioprinted scaffold is proposed, containing a calcitonin gene‐related peptide (CGRP) and the β‐adrenergic receptor blocker propranolol (PRN) as a new method to achieve effective repair of bone defects. By leveraging the neuromodulation mechanism of bone regeneration, CGRP and PRN loaded mesoporous silica nanoparticles are added into a hybrid bio‐ink, which initially contains gelatin methacrylate, Poly (ethylene glycol) diacrylate and bone marrow mesenchymal stem cells (BMSCs). Subsequently, the optimized bio‐ink is used for 3D bioprinting to create a composite scaffold with a pre‐designed micro‐nano hierarchical structure. The migration and tube formation of human umbilical vein endothelial cells (HUVECs) can be promoted by the scaffold, which is beneficial to the formation of a new capillary network during the bone repair process. With the release of CGRP from the scaffold, the secretion of neuropeptides by sensory nerves is simulated. Meanwhile, the release of PRN can inhibit the binding process of catecholamine to β‐adrenergic receptor, co‐promoting the osteogenic differentiation of BMSCs with CGRP and silicon ions, which will effectively enhance bone repair of a critical‐sized cranial defect in a rat model. In conclusion, this study provides a promising strategy for bone defect repair by understanding the neuromodulatory mechanisms during bone regeneration.

show abstract

Ion‐Engineered Microcryogels via Osteogenesis‐Angiogenesis Coupling and Inflammation Reversing Augment Vascularized Bone Regeneration

Wang,

Pang

et al. 2024

Adv Funct Materials

View full text Add to dashboard Cite

Native bone inherently requires a balanced ionic microenvironment to maintain bone homeostasis. Hence, scaffolds designed for the sustained release of therapeutic ions into bone defects hold great promise for bone regeneration. Magnesium (Mg) and silicon (Si) are essential elements, which play crucial roles in the process of bone regeneration, impacting immunomodulation, angiogenesis, and osteogenesis. Herein, porous cryogel‐type organic–inorganic composite microspheres are developed as injectable microscaffolds (denoted as GMN). GMN enables sustained release of Mg/Si ions at an optimized ratio, achieving the most significant synergistic effect on vascularized bone regeneration. Various conditioned media are obtained to explore angiogenesis‐osteogenesis coupling, as well as the crosstalk between bone marrow mesenchymal stromal cells (BMSCs) and macrophages. Meanwhile, autocrine and paracrine effects simultaneously play synergistic modulating functions in determining cell fates under the guidance of Mg/Si ions and biofactors secreted by cells. Overall, the Mg/Si ion‐engineering microscaffolds create a conducive microenvironment to efficiently augment the regeneration of vascularized bone tissue in vivo, offering a versatile platform for tissue engineering.

show abstract

Stratified-structural hydrogel incorporated with magnesium-ion-modified black phosphorus nanosheets for promoting neuro-vascularized bone regeneration

Cited by 71 publications

References 39 publications

Translating Material Science into Bone Regenerative Medicine Applications: State-of-The Art Methods and Protocols

Translating Material Science into Bone Regenerative Medicine Applications: State-of-The Art Methods and Protocols

Bioprinted Scaffold Remodels the Neuromodulatory Microenvironment for Enhancing Bone Regeneration

Ion‐Engineered Microcryogels via Osteogenesis‐Angiogenesis Coupling and Inflammation Reversing Augment Vascularized Bone Regeneration

Contact Info

Product

Resources

About