Strategy of combining offline 2D LC‐MS with LC‐DIA‐MS/MS to accurately identify chemical compounds and for quality control of Dioscorea septemloba Thunb

Abstract

Introduction: Dioscorea septemloba Thunb. (DST), the rhizome of Dioscorea spongiosa

Efficient discovery of active isolates from Dioscorea spongiosa by the combination of bioassay‐guided macroporous resin column chromatography and high‐speed counter‐current chromatography

“…Mizuno et W. L. Zhao, known as Mianbixie in China, is a well-known traditional Chinese medicine belonging to the Dioscoreaceae family [1]. This herb medicine is mainly planted in Zhejiang, Fujian, and Jiangxi Provinces, China, which has been famous for its application of the treatments of gonorrhea white turbid, excessive leucorrhea, damp heat sore poison, waist and knee arthralgia, and so on [2][3][4][5][6]. Chemical studies of D. spongiosa have indicated the main chemical constituents as steroids, triterpenoid saponins, diarylheptanes, flavonoids, and lignans, with the first two kinds being the major ones [7][8][9][10], which showed various pharmacological activities including lowering uric acid, anti-diabetic, anti-inflammation, anti-tumor, anti-atherosclerosis, regulating blood lipids, anti-oxidation, antibacterial [11].…”

“…Mizuno et W. L. Zhao, known as Mianbixie in China, is a well‐known traditional Chinese medicine belonging to the Dioscoreaceae family [1]. This herb medicine is mainly planted in Zhejiang, Fujian, and Jiangxi Provinces, China, which has been famous for its application of the treatments of gonorrhea white turbid, excessive leucorrhea, damp heat sore poison, waist and knee arthralgia, and so on [2–6]. Chemical studies of D .…”