Strategies to simplify operation procedures for applying labeled antibody-based immunosensors to point-of-care testing

Kimura, Hayato; Asano, Ryutaro

doi:10.1016/j.ab.2022.114806

Cited by 5 publications

(3 citation statements)

References 96 publications

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“…The ideal approach for protein detection should be sensitive, specific, robust, and rapid, which does not call for expensive apparatus and professional users . Unfortunately, conventional approaches for PCSK9 quantification mainly relied on Western blot and enzyme-linked immunosorbent assay (ELISA), − which were limited by low sensitivity and cumbersome operation. − Electrochemical sensors have been developed for the ultrasensitive assay of PCSK9. ,, For example, Yu et al fabricated an electrochemical immunosensor to detect PCSK9, using nanomaterials with good conductivity and glassy carbon electrodes .…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Homogeneous Detection of PCSK9 and Efficacy Monitoring of the PCSK9 Inhibitor Based on Proximity Hybridization-Dependent Chemiluminescence Imaging Immunoassay

Yang

Cao³

et al. 2023

Anal. Chem.

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Accurate quantification of proprotein convertase subtilisin/kexin type 9 (PCSK9) in serum before and after the medication is helpful in grasping the evolution of PCSK9-related disease and evaluating the efficacy of PCSK9 inhibitors. Conventional approaches for PCSK9 quantification suffered from complicated operations and low sensitivity. By integrating stimuli-responsive mesoporous silica nanoparticles, dual-recognition proximity hybridization, and T7 exonuclease-assisted recycling amplification, a novel homogeneous chemiluminescence (CL) imaging approach was proposed for ultrasensitive and convenient immunoassay of PCSK9. Owing to the intelligent design and signal amplification property, the whole assay was conducted without separation and rinsing, significantly simplifying the procedure and eliminating the errors associated with the professional operation; meanwhile, it showed linear ranges over 5 orders of magnitude and detection limit as low as 0.7 pg mL–1. Parallel testing was allowed due to the imaging readout, which brought a maximum throughput of 26 tests h–1. The proposed CL approach was applied to analyze PCSK9 from hyperlipidemia mice before and after the intervention of the PCSK9 inhibitor. Serum PCSK9 levels in the model group and the intervention group could be distinguished efficiently. The results were reliable compared to commercial immunoassay results and histopathologic findings. Thus, it could facilitate the monitoring of the serum PCSK9 level and the lipid-lowering effect of the PCSK9 inhibitor, showing promising potential in bioanalysis and pharmaceuticals.

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Section: Introductionmentioning

confidence: 99%

Ultrasensitive Homogeneous Detection of PCSK9 and Efficacy Monitoring of the PCSK9 Inhibitor Based on Proximity Hybridization-Dependent Chemiluminescence Imaging Immunoassay

Yang

Cao³

et al. 2023

Anal. Chem.

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“…Enzyme-linked immunosorbent assay (ELISA) is widely used for detecting and quantifying target analytes in biological and clinical samples. Advancements in assay chemistry, microfluidics, and automation have been implemented in an effort to simplify the ELISA workflow [1][2][3][4][5] and several commercially available ELISAs are now based on a 90-minute, 3-step, single-wash protocol (e.g. SimpleStep ELISA from Abcam or the Quantikine ELISA from R&D Systems).…”

Section: Introductionmentioning

confidence: 99%

“Glow ELISA”: sensitive immunoassay with minimal equipment and stable reagents

Vu,

Brosamer,

McDaniel

et al. 2023

Analyst

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“…Optical and electrochemical detection systems are widely used to create miniature devices for protein detection [14,15]. Optical detection methods, which are considered very sensitive and specific, are based on a change in the phase, amplitude, polarization or frequency of incoming light in response to biorecognition processes.…”

Section: Introductionmentioning

confidence: 99%

Impedimetric Biosensor Coated with Zinc Oxide Nanorods Synthesized by a Modification of the Hydrothermal Method for Antibody Detection

et al. 2023

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Impedimetric biosensors are used for detecting a wide range of analytes. The detection principle is a perspective for the development of new types of analytical devices for biomolecular diagnosis of diseases. Of particular interest are biosensors with very high sensitivities, capable of detecting trace amounts of biomarkers or drugs in biological fluids. Impedimetric biosensors possess a potential for increased sensitivity, since their electrodes can be modified with nanostructured materials, in particular zinc oxide. In this work, a miniature biosensor with an array of zinc oxide nanorods synthesized by the hydrothermal method has been created. Protein A was immobilized on the resulting structure, which was previously tested for binding to omalizumab by capillary electrophoresis. Using impedance spectroscopy, it was possible to detect the binding of omalizumab at concentrations down to 5 pg/mL. The resulting structures are suitable for creating reusable biosensor systems, since ZnO-coated electrodes are easily cleaned by photocatalytic decomposition of the bound molecules. The biosensor is promising for use in Point-of-Care systems designed for fast, multimodal detection of molecular markers of a wide range of diseases.

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Strategies to simplify operation procedures for applying labeled antibody-based immunosensors to point-of-care testing

Cited by 5 publications

References 96 publications

Ultrasensitive Homogeneous Detection of PCSK9 and Efficacy Monitoring of the PCSK9 Inhibitor Based on Proximity Hybridization-Dependent Chemiluminescence Imaging Immunoassay

Ultrasensitive Homogeneous Detection of PCSK9 and Efficacy Monitoring of the PCSK9 Inhibitor Based on Proximity Hybridization-Dependent Chemiluminescence Imaging Immunoassay

“Glow ELISA”: sensitive immunoassay with minimal equipment and stable reagents

Impedimetric Biosensor Coated with Zinc Oxide Nanorods Synthesized by a Modification of the Hydrothermal Method for Antibody Detection

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