Strategies to promote permeation and vectorization, and reduce cytotoxicity of metal complex luminophores for bioimaging and intracellular sensing

Holden, Lorcan; Burke, Christopher S.; Cullinane, David; Keyes, Tia E.

doi:10.1039/d1cb00049g

Cited by 28 publications

(28 citation statements)

References 183 publications

(228 reference statements)

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“…The use of nanocarriers [ 95 – 98 ], liposomes [ 99 ], dendrimers [ 100 ], sugars/carbohydrates [ 101 , 102 ], polyethyleneglycol (PEG) chains [ 81 , 82 ], vitamins [ 103 – 105 ], antibodies [ 106 ], lipophilic moieties such as triphenylphosphonium (TPP) [ 107 ], amino acids [ 108 ] and cell-penetrating peptides (CPPs) [ 81 , 109 – 111 ] has also been shown to increase solubility and improve membrane permeability, facilitating reliable uptake of complexes within cells for a range of applications. Recent reviews describe the preparation and application of ruthenium bioconjugates [ 112 ] and vectorisation strategies of metal complex luminophores [ 3 ].…”

Section: Rationale For Peptide Conjugation To Transition Row Metal Co...mentioning

confidence: 99%

“…A challenge that has traditionally impeded the application of metal complexes as bioimaging and biosensing probes is the poor uptake of such materials into cells. This has been widely overcome in recent years with a variety of strategies involving modification of the physicochemical properties of the complex or bioconjugation [ 3 ]. Conjugation of complexes to cell-penetrating and signal peptides specifically, has proven to be a particularly attractive and reliable method for achieving efficient cellular uptake without the use of permeabilisation agents.…”

Section: Introductionmentioning

confidence: 99%

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Metal Peptide Conjugates in Cell and Tissue Imaging and Biosensing

2022

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Metal complex luminophores have seen dramatic expansion in application as imaging probes over the past decade. This has been enabled by growing understanding of methods to promote their cell permeation and intracellular targeting. Amongst the successful approaches that have been applied in this regard is peptide-facilitated delivery. Cell-permeating or signal peptides can be readily conjugated to metal complex luminophores and have shown excellent response in carrying such cargo through the cell membrane. In this article, we describe the rationale behind applying metal complexes as probes and sensors in cell imaging and outline the advantages to be gained by applying peptides as the carrier for complex luminophores. We describe some of the progress that has been made in applying peptides in metal complex peptide-driven conjugates as a strategy for cell permeation and targeting of transition metal luminophores. Finally, we provide key examples of their application and outline areas for future progress.

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Section: Rationale For Peptide Conjugation To Transition Row Metal Co...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metal Peptide Conjugates in Cell and Tissue Imaging and Biosensing

2022

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“…[1][2][3][4][5][6][7][8][9][10], and a broad range of applications in the fields of luminescence chemosensing, [11][12][13] photocatalysis, [14][15][16] sensitisation, [17][18][19] selfassembled materials, 20 and bioimaging. [20][21][22][23] Although pure organic light emitters are predominantly fluorescent (triplet excitons formed are deactivated by thermal processes), the presence of strong spin-orbit coupling in transition metal complexes upholds the intersystem crossing from the singlet to the triplet state enabling phosphorescent relaxation. An electroluminescent device doped with a transition metal compound may form up to 25% singlet and 75% triplet excitons resulting in nearly quantitative luminescence efficiencies.…”

Section: Introductionmentioning

confidence: 99%

Tuning the luminescence of transition metal complexes with acyclic diaminocarbene ligands

Kinzhalov

Grachova

Luzyanin

2022

Inorg. Chem. Front.

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“…Numerous luminescent substances exist that can be used in various applications, such as bioimaging probes [1,2], biosensors [2,3], light-emitting diodes [4][5][6], and lighting devices [4][5][6]. Luminescent materials can be divided into two classes according to the physical state of the luminophores: solution-state luminophores and solid-state luminophores.…”

Section: Introductionmentioning

confidence: 99%

Unsymmetrical Hexafluorocyclopentene-Linked Twisted π-Conjugated Molecules as Dual-State Emissive Luminophores

et al. 2021

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Dual-state emissive (DSE) luminophores, which can luminesce both in solution and in solid states, have recently attracted significant attention because of their broad applications. However, their development is difficult due to the difference in molecular design between solution- and solid-state luminophores. In this study, DSE luminophores based on unsymmetrical hexafluorocyclopentene-linked twisted π-conjugated structures carrying various substituents to tune the electron-density were designed and synthesized in a single-step reaction from heptafluorocyclopentene or perfluoro-1,2-diphenylcyclopentene derivatives. The twisted π-conjugated luminophores exhibited absorption in the UV region at approximately 330 nm, along with several signals in the high-energy region. Upon irradiating the luminophore solution (wavelength 330 nm), light-green to yellow photoluminescence (PL) was observed in the range of 422–471 nm with high PL efficiency. Theoretical calculations revealed that excitation from ground to excited states altered the structural shape of the luminophores from twisted to planar, leading to red-shifted PL and high PL efficiency (ΦPL). The intense blue PL exhibited by the luminophores in the crystalline state was attributed to their twisted molecular structures that suppressed non-radiative deactivation via the effective blocking of π/π stacking interactions.

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Strategies to promote permeation and vectorization, and reduce cytotoxicity of metal complex luminophores for bioimaging and intracellular sensing

Abstract: Transition metal luminophores are emerging as important tools for intracellular imaging and sensing. Their putative suitability for such applications has long been recognised but poor membrane permeability and cytotoxicity were...

Cited by 28 publications

References 183 publications

Metal Peptide Conjugates in Cell and Tissue Imaging and Biosensing

Metal Peptide Conjugates in Cell and Tissue Imaging and Biosensing

Tuning the luminescence of transition metal complexes with acyclic diaminocarbene ligands

Unsymmetrical Hexafluorocyclopentene-Linked Twisted π-Conjugated Molecules as Dual-State Emissive Luminophores

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