Strategies to optimize the biocompatibility of iron oxide nanoparticles – “SPIONs safe by design”

Janko, Christina; Zaloga, Jan; Pöttler, Marina; Dürr, Stephan; Eberbeck, Dietmar; Tietze, Rainer; Lyer, Stefan; Alexiou, Christoph

doi:10.1016/j.jmmm.2016.09.034

Cited by 50 publications

(27 citation statements)

References 19 publications

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“…Two hundred micrograms per milliliters SPIONs were suspended in plasma, PBS or medium containing 10% serum and incubated for 10 min. We observed that the SPIONs exhibited different colloidal stabilities, with SPION LA1 and SPION LA2 forming clusters after incubation in the presence of PBS (Figure 1A ); incubation with plasma or medium containing 10% serum did not induce clusters due to the formation of stabilizing protein coronae (Figures 1B , 2A ) ( 11 , 12 ).…”

Section: Resultsmentioning

confidence: 99%

“…For SPION DEX dedicated for magnetic resonance imaging we previously proofed the absence of complement activation in vitro and complement-activation related pseudoallergy (CARPA) in vivo , probably due to tight coverage of the iron surface by complete cross-linking of the dextran shell ( 8 , 34 ), indicating the importance of proper coatings for biocompatibility. For SPIONs dedicated for magnetic drug targeting (SPION LA−HSA ) we previously showed that an artificial albumin protein corona can colloidally stabilize iron oxide nanoparticles and increase their biocompatibility ( 11 , 35 , 36 ).…”

Section: Discussionmentioning

confidence: 99%

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Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps

et al. 2018

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If foreign particles enter the human body, the immune system offers several mechanisms of response. Neutrophils forming the first line of the immune defense either remove pathogens by phagocytosis, inactivate them by degranulation or release of reactive oxygen species or immobilize them by the release of chromatin decorated with the granular proteins from cytoplasm as neutrophil extracellular traps (NETs). Besides viable microbes like fungi, bacteria or viruses, also several sterile inorganic particles including nanoparticles reportedly activate NET formation. The physicochemical nanoparticle characteristics fostering NET formation are still elusive. Here we show that agglomerations of non-stabilized superparamagnetic iron oxide nanoparticles (SPIONs) induce NET formation by isolated human neutrophils, in whole blood experiments under static and dynamic conditions as well as in vivo. Stabilization of nanoparticles with biocompatible layers of either human serum albumin or dextran reduced agglomeration and NET formation by neutrophils. Importantly, this passivation of the SPIONs prevented vascular occlusions in vivo even when magnetically accumulated. We conclude that higher order structures formed during nanoparticle agglomeration primarily trigger NET formation and the formation of SPION-aggregated NET-co-aggregates, whereas colloid-disperse nanoparticles behave inert and are alternatively cleared by phagocytosis.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps

et al. 2018

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“…Damage of the erythrocyte membrane leads to release of hemoglobin, which is toxic when present in the extracellular space and can cause life-threatening situations [ 32 ]. To analyze hemocompatibility, we performed quantitative colorimetric determination of free hemoglobin of erythrocytes co-incubated with SPIONs as previously described [ 33 ]. Compared to the positive control (Triton X-100), the incubation of blood from three donors with SPIONs did not induce release of hemoglobin, even in the highest tested SPION concentration ( Figure 1 B).…”

Section: Resultsmentioning

confidence: 99%

Mitoxantrone-Loaded Nanoparticles for Magnetically Controlled Tumor Therapy–Induction of Tumor Cell Death, Release of Danger Signals and Activation of Immune Cells

et al. 2020

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Stimulating the patient`s immune system represents a promising therapeutic strategy to fight cancer. However, low immunogenicity of the tumor cells within an immune suppressive milieu often leads to weak anti-tumor immune responses. Additionally, the immune system may be impaired by accompanying aggressive chemotherapies. We show that mitoxantrone, bound to superparamagnetic iron oxide nanoparticles (SPIONs) as the transport system, can be magnetically accumulated in adherent HT-29 colon carcinoma cells, thereby inducing the same cell death phenotype as its soluble counterpart, a chemotherapeutic agent and prototypic inductor of immunogenic cell death. The nanoparticle-loaded drug induces cell cycle stop, apoptosis and secondary necrosis in a dose- and time-dependent manner comparable to the free drug. Cell death was accompanied by the release of interleukin-8 and damage-associated molecular patterns (DAMPs) such as HSP70 and ATP, which fostered chemotactic migration of monocytes and maturation of dendritic cells. We furthermore ensured absence of endotoxin contaminations and compatibility with erythrocytes and platelets and investigated the influence on plasma coagulation in vitro. Summarizing, with magnetic enrichment, mitoxantrone can be accumulated at the desired place, sparing healthy peripheral cells and tissues, such as immune cells. Conserving immune competence in cancer patients in the future might allow combined therapeutic approaches with immune therapies (e.g. checkpoint inhibitors).

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“…The nano-gold structures drastically decreased the performance of the yeast-based MFC, as the gold nanostructure poisoned the yeast cell [38]. Iron oxide nanoparticles are another type of transition metal nanoparticles that are cheap, available, biocompatible, and already investigated in different biomedical applications [58][59][60]. The application of iron oxide nanoparticles prepared by a chemical route followed by depositing the nanoparticles on the surface of a graphite electrode, resulted in the increased performance of the MFC, due to the improvement of the electron transfer between the nanoparticles and the microorganisms [61].…”

Section: Introductionmentioning

confidence: 99%

A Carbon-Cloth Anode Electroplated with Iron Nanostructure for Microbial Fuel Cell Operated with Real Wastewater

et al. 2020

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Microbial fuel cell (MFC) is an emerging method for extracting energy from wastewater. The power generated from such systems is low due to the sluggish electron transfer from the inside of the biocatalyst to the anode surface. One strategy for enhancing the electron transfer rate is anode modification. In this study, iron nanostructure was synthesized on a carbon cloth (CC) via a simple electroplating technique, and later investigated as a bio-anode in an MFC operated with real wastewater. The performance of an MFC with a nano-layer of iron was compared to that using bare CC. The results demonstrated that the open-circuit voltage increased from 600 mV in the case of bare CC to 800 mV in the case of the iron modified CC, showing a 33% increase in OCV. This increase in OCV can be credited to the decrease in the anode potential from 0.16 V vs. Ag/AgCl in the case of bare CC, to −0.01 V vs. Ag/AgCl in the case of the modified CC. The power output in the case of the modified electrode was 80 mW/m2—two times that of the MFC using the bare CC. Furthermore, the steady-state current in the case of the iron modified carbon cloth was two times that of the bare CC electrode. The improved performance was correlated to the enhanced electron transfer between the microorganisms and the iron-plated surface, along with the increase of the anode surface- as confirmed from the electrochemical impedance spectroscopy and the surface morphology, respectively.

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Strategies to optimize the biocompatibility of iron oxide nanoparticles – “SPIONs safe by design”

Cited by 50 publications

References 19 publications

Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps

Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps

Mitoxantrone-Loaded Nanoparticles for Magnetically Controlled Tumor Therapy–Induction of Tumor Cell Death, Release of Danger Signals and Activation of Immune Cells

A Carbon-Cloth Anode Electroplated with Iron Nanostructure for Microbial Fuel Cell Operated with Real Wastewater

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