Strategies to Increase Expression and Accumulation of Recombinant Proteins

Saberianfar, Reza; Menassa, Rima

doi:10.1002/9781118801512.ch6

Cited by 4 publications

(2 citation statements)

References 105 publications

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“…Moreover, in many cases, the transcriptional nature of constitutive promoters often leads to poor growth of the host plants and affects yields, whereas seed-specific promoters restrict the accumulation of heterologous proteins in seed tissue. Therefore, in addition to the increased stability of the recombinant protein, the vegetative organs are preserved from the undesirable effects of accumulated foreign proteins [ 75 , 76 , 77 , 78 ]. In this context, our transgenic barley plants, which stably expressed rhLL-37 driven by the barley endosperm-specific B1 hordein gene promoter, illustrate the functional expression of transgenes in progeny up to the T6 generation.…”

Section: Discussionmentioning

confidence: 99%

Long-Lasting Stable Expression of Human LL-37 Antimicrobial Peptide in Transgenic Barley Plants

Mirzaee¹,

Holásková²,

Mičúchová³

et al. 2021

Antibiotics

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Antimicrobial peptides play a crucial role in the innate immune system of multicellular organisms. LL-37 is the only known member of the human cathelicidin family. As well as possessing antibacterial properties, it is actively involved in various physiological responses in eukaryotic cells. Accordingly, there is considerable interest in large-scale, low-cost, and microbial endotoxin-free production of LL-37 recombinant peptides for pharmaceutical applications. As a heterologous expression biofactory, we have previously obtained homologous barley (Hordeum vulgare L.) as an attractive vehicle for producing recombinant human LL-37 in the grain storage compartment, endosperm. The long-term stability of expression and inheritance of transgenes is necessary for the successful commercialization of recombinant proteins. Here, we report the stable inheritance and expression of the LL-37 gene in barley after six generations, including two consecutive seasons of experimental field cultivation. The transgenic plants showed normal growth and remained fertile. Based on the bacteria viability test, the produced peptide LL-37 retained high antibacterial activity.

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Section: Discussionmentioning

confidence: 99%

Long-Lasting Stable Expression of Human LL-37 Antimicrobial Peptide in Transgenic Barley Plants

Mirzaee¹,

Holásková²,

Mičúchová³

et al. 2021

Antibiotics

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“…The following plant systems for expression of foreign proteins are exploited: transgenic plants with nuclear/chloroplast transformed genomes, cell suspension cultures, and transient expression. The recombinant proteins can be targeted to various subcellular organelles or compartments such as ER, apoplast, cytosol, and chloroplasts to find the most appropriate cellular surroundings for their accumulation [85].…”

Section: Heterologous Expression Of Therapeutic Proteins In Plantsmentioning

confidence: 99%

Medicinal Plants: Guests and Hosts in the Heterologous Expression of High-Value Products

Vasil'ev

2021

Planta Med

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Medicinal plants play an important dual role in the context of the heterologous expression of high-value pharmaceutical products. On the one hand, the classical biochemical and modern omics approaches allowed for the discovery of various genes encoding biosynthetic pathways in medicinal plants. Recombinant DNA technology enabled introducing these genes and regulatory elements into host organisms and enhancing the heterologous production of the corresponding secondary metabolites. On the other hand, the transient expression of foreign DNA in plants facilitated the production of numerous proteins of pharmaceutical importance. This review summarizes several success stories of the engineering of plant metabolic pathways in heterologous hosts. Likewise, a few examples of recombinant protein expression in plants for therapeutic purposes are also highlighted. Therefore, the importance of medicinal plants has grown immensely as sources for valuable products of low and high molecular weight. The next step ahead for bioengineering is to achieve more success stories of industrial-scale production of secondary plant metabolites in microbial systems and to fully exploit plant cell factoriesʼ commercial potential for recombinant proteins.

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Molecular Farming for the Production of Recombinant Pharmaceutical Proteins in Plants

Fidan,

Secgin

2024

Concepts and Strategies in Plant Sciences

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Strategies to Increase Expression and Accumulation of Recombinant Proteins

Cited by 4 publications

References 105 publications

Long-Lasting Stable Expression of Human LL-37 Antimicrobial Peptide in Transgenic Barley Plants

Long-Lasting Stable Expression of Human LL-37 Antimicrobial Peptide in Transgenic Barley Plants

Medicinal Plants: Guests and Hosts in the Heterologous Expression of High-Value Products

Molecular Farming for the Production of Recombinant Pharmaceutical Proteins in Plants

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