Strategies to improve the efficiency of celiac disease diagnosis in the laboratory

González, Delia Almeida; Armas, Laura García de; Rodríguez, Itahisa Marcelino; Almeida, Ana Arencibia; García, Miriam García; Gannar, Fadoua; León, Antonio Cabrera de

doi:10.1016/j.jim.2017.07.007

Cited by 3 publications

(1 citation statement)

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“…[5][6][7] CD may occur in adults and children, 8 with a female/male ratio of 3:1. 9 About 90% of the CD patients carry a major histocompatibility complex class II molecule, human leukocyte antigen (HLA) variant DQ2 and most of the remainder of patients carry an HLA variant named DQ8. 10 The clinical presentation of CD ranges from asymptomatic form to a symptomatic which could represent a general malabsorption (typical or classical) or extra intestinal symptoms (Atypical or symptomatic).…”

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confidence: 99%

Evaluation of T-Bet immunostaining in the counting of intraepithelial lymphocytes in celiac disease

Al-Araji

Omran

Ahmed

et al. 2020

J. Contemp. Med. Sci.

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Objective: In this study, we aimed to evaluate CD3, T-bet and GATA3 staining of intraepithelial lymphocytes (IELs) in comparison to the routine H&E stains in celiac disease. Methods: a total of 50 patients, in whom celiac disease was diagnosed based on a combination of clinicopathological features, were enrolled in the study. Duodenal biopsied tissues were processed routinely into formalin fixed paraffin embedded (FFPE) blocks. Sections were prepared and stained with H&E and each of CD3, T-bet and GATA3. A number of histological criteria were measured to calculate the Marsh score. The results were analyzed using the IBM SPSS analytic software. Results: a positive correlation was found between the count of T-bet stained cells and the increase of intraepithelial lymphocytes (P-value = 0.001). In addition, low count of GATA3 stained cells was seen in almost all cases. The count of GATA3 stained cells was not affected by the increase in IELs count. Conclusions: the majority of increased IELs were stained with T-bet. Whereas in normal IELs count is less than half the IELs were stained with T-bet. This would indicate that T-bet immunostaining is a potential alternative to H&E and/or CD3 based counting of IELs.

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