Strategies to bioengineer aptamer-driven nanovehicles as exceptional molecular tools for targeted therapeutics: A review

Thevendran, Ramesh; Sarah, Shigdar; Tang, Thean‐Hock; Citartan, Marimuthu

doi:10.1016/j.jconrel.2020.04.051

Cited by 31 publications

(22 citation statements)

References 168 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, aptamer provides a promising recognition platform in targeted therapeutics. [152][153][154] There are several successful attempts to targeting cancer cells by modifying Au NCs or their nanocomposites with aptamers. For example, AS1411 is ssDNA aptamer with high binding affinity to nucleolin (NCL).…”

Section: Aptamer Targetingmentioning

confidence: 99%

Engineering Metal Nanoclusters for Targeted Therapeutics: From Targeting Strategies to Therapeutic Applications

Lin

Goswami

Xue

et al. 2021

Adv Funct Materials

View full text Add to dashboard Cite

Metal nanoclusters (NCs) have recently attracted great interest in biomedical applications due to their ultrasmall size, good biocompatibility, and unique molecule-like physical and chemical properties. Metal NCs can be rationally designed and integrated with various targeting moieties to achieve unique physicochemical properties and functions. For therapeutic applications, these multifunctional surface-modified NCs can provide distinctive advantages over native metal NCs, such as improved therapeutic effects and reduced side effects. In this review, the design principles of targeting strategies for metal NCs and their composites, including passive and active targeting, and physical and chemical targeting are first discussed. The authors then focus on the recent achievements in the application of metal NCs in targeted therapeutics, including chemotherapy, phototherapy, and radiotherapy. Finally, the authors' perspectives on the challenges and opportunities of developing metal NCs in targeted therapeutics, further paving their way for potential clinical applications are provided.

show abstract

Section: Aptamer Targetingmentioning

confidence: 99%

Engineering Metal Nanoclusters for Targeted Therapeutics: From Targeting Strategies to Therapeutic Applications

Lin

Goswami

Xue

et al. 2021

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…More specifically, aptamers are single-stranded DNA or RNA molecules that bind proteins with high specificity because of their stable three-dimensional shapes. 57 DNA and RNA aptamers are typically generated through the process of systematic evolution of ligands by exponential enrichment (SELEX), which allows the selection of 20-to 100-nt aptamers from libraries, binding various protein families with high affinity to modulate their functions 58 (Figure S3).…”

Section: Aptamermentioning

confidence: 99%

“…In addition, by combining with other types of therapeutic agents, aptamers can be utilized as delivery vehicles for siRNAs, enzymes, and anticancer drugs. 58 Prostate-specific membrane antigen (PSMA) is an overexpressed receptor on the surface of prostate cancer cells and tumor vascular endothelium, which offers a binding site for aptamers. Meanwhile, siRNAs are responsible for the expression of survival genes.…”

Section: Aptamermentioning

confidence: 99%

“…In addition, high stability at extreme temperatures and in other harmful environments, a simple modification process, an inexpensive synthesis process, and low variation between batches render aptamer-based delivery systems achievable and successful. 58 Although aptamers have shown good targeting performance in vitro, there are still many factors to be considered in order to apply aptamers in vivo. Aptamers bind to their targets by forming secondary or tertiary structures, and the formation of the correct structure can be further affected by the environment surrounding the target, such as temperature, pH, and ionic strength.…”

Section: Aptamermentioning

confidence: 99%

See 1 more Smart Citation

The development and improvement of ribonucleic acid therapy strategies

Zhao

Shu

Liu

2021

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

The biological understanding of RNA has evolved since the discovery of catalytic RNAs in the early 1980s and the establishment of RNA interference (RNAi) in the 1990s. RNA is no longer seen as the simple mid-product between transcription and translation, but potential molecules to be developed as RNA therapeutic drugs. RNA-based therapeutic drugs have gained recognition due to their ability to regulate gene expression and perform cellular functions. Various nucleobase, backbone, and sugar-modified oligonucleotides have been synthesized, as natural oligonucleotides with some limitations such as poor low nuclease resistance, binding affinity, poor cellular uptake and toxicity, which affect their use as RNA therapeutic drugs. In this review, we briefly discuss different RNA therapeutic drugs and the internal connection, including antisense oligonucleotides, siRNA and miRNA, aptamers, saRNA, and RNA vaccines, we also discuss the important roles of RNA vaccines and their use in the fight against COVID-19. In addition, various chemical modifications and delivery systems used to improve the performance of RNA therapeutic drugs and overcome their limitations are discussed.

show abstract

“…Aptamers are folded nucleic acid strands capable of binding to different target molecules with high programmability and low immunogenicity. 100 In one study, cholesterol modified aptamer AS1411 conferred exosome enhanced tumor targeting in an MDA-MB -231-xenografted mouse model due to specific binding of AS1411 aptamers to nucleolin on breast cancer cells. 101 Surface modification of exosomes also allows for 2-in-1 or all-in-1 strategies to achieve tumor specific-killing.…”

mentioning

confidence: 99%

<p>Advances in Exosome-Based Drug Delivery and Tumor Targeting: From Tissue Distribution to Intracellular Fate</p>

Shao

Zaro

Shen

2020

IJN

169

100

View full text Add to dashboard Cite

Exosomes or small extracellular vesicles are considered a new generation of bioinspired-nanoscale drug delivery system (DDS). Endogenous exosomes function as signalosomes since they convey signals via ligands or adhesion molecules located on the exosomal membrane, or packaged inside the exosome. Recently, exosome membrane modification, therapeutic payloads encapsulation, and modulation of in vivo disposition of exosomes have been extensively investigated, among which significant advances have been made to optimize exosome-mediated delivery to solid tumors. Exosomes, specifically tumor cell-derived exosomes, are presumed to have tumor-preferential delivery due to the homotypic features. However, quality attributes that dictate the tissue distribution, cell typeselective uptake, and intracellular payload release of the administered exosomes, as well as the spatiotemporal information regarding exosome fate in vivo, remain to be further investigated. This review summarizes recent advances in developing exosomes as drug delivery platforms with a focus on tumor targeting. The pharmacokinetic features of naive exosomes and factors influencing their intracellular fate are summarized. Recent strategies to improve tumor targeting of exosomes are also reviewed in the context of the biological features of tumor and tumor microenvironment (TME). Selected approaches to augment tumor tissue deposition of exosomes, as well as methods to enhance intracellular payload delivery, are summarized with emphasis on the underlying mechanisms (eg, passive or active targeting, endosomal escape, etc.). In conclusion, this review highlights recently reported tumortargeting strategies of exosome-based drug delivery, and it's in the hope that multiple approaches might be employed in a synergistic combination in the development of exosomebased cancer therapy.

show abstract

Strategies to bioengineer aptamer-driven nanovehicles as exceptional molecular tools for targeted therapeutics: A review

Cited by 31 publications

References 168 publications

Engineering Metal Nanoclusters for Targeted Therapeutics: From Targeting Strategies to Therapeutic Applications

Engineering Metal Nanoclusters for Targeted Therapeutics: From Targeting Strategies to Therapeutic Applications

The development and improvement of ribonucleic acid therapy strategies

<p>Advances in Exosome-Based Drug Delivery and Tumor Targeting: From Tissue Distribution to Intracellular Fate</p>

Contact Info

Product

Resources

About