Strategies related to light quality and temperature to improve lutein production of marine microalga Chlamydomonas sp.

Zhao, Xurui; Ma, Ruijuan; Liu, Xiaoting; Ho, Shih‐Hsin; Xie, Youping; Chen, Jianfeng

doi:10.1007/s00449-018-2047-4

Cited by 36 publications

(19 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…JSC4 under different environmental conditions for lutein production and concluded that the optimal lutein content was obtained under blue light and a lower temperature of 20-25 • C. Schüler et al [49] also examined different environmental factors on marine microalga Tetraselmis sp. CTP4 for carotenoid biosynthesis and examined that lutein amount increased 1.5-fold under higher light intensities (170 and 280 µmol photons m −2 s −1 ), but in contrast to Zhao et al [48], found a temperature increase from 20 to 35 • C, after only two days at a light intensity of 170 µmol photons m −2 s −1 , yielded the highest lutein productivity (3.17 ± 0.18 mg g −1 dry weight biomass). Ma et al [50] also examined light stress on the marine microalga Chlamydomonas sp.…”

Section: Microalgae Cultivation For Commercial Lutein Production and mentioning

confidence: 92%

“…Zhao et al [48] examined the marine microalga Chlamydomonas sp. JSC4 under different environmental conditions for lutein production and concluded that the optimal lutein content was obtained under blue light and a lower temperature of 20-25 • C. Schüler et al [49] also examined different environmental factors on marine microalga Tetraselmis sp.…”

Section: Microalgae Cultivation For Commercial Lutein Production and mentioning

confidence: 99%

See 1 more Smart Citation

Marine Microalgae for Potential Lutein Production

2020

View full text Add to dashboard Cite

Lutein is particularly known to help maintain normal visual function by absorbing and attenuating the blue light that strikes the retina in our eyes. The effect of overexposure to blue light on our eyes due to the excessive use of electronic devices is becoming an issue of modern society due to insufficient dietary lutein consumption through our normal diet. There has, therefore, been an increasing demand for lutein-containing dietary supplements and also in the food industry for lutein supplementation in bakery products, infant formulas, dairy products, carbonated drinks, energy drinks, and juice concentrates. Although synthetic carotenoid dominates the market, there is a need for environmentally sustainable carotenoids including lutein production pathways to match increasing consumer demand for natural alternatives. Currently, marigold flowers are the predominant natural source of lutein. Microalgae can be a competitive sustainable alternative, which have higher growth rates and do not require arable land and/or a growth season. Currently, there is no commercial production of lutein from microalgae, even though astaxanthin and β-carotene are commercially produced from specific microalgal strains. This review discusses the potential microalgae strains for commercial lutein production, appropriate cultivation strategies, and the challenges associated with realising a commercial market share.

show abstract

Section: Microalgae Cultivation For Commercial Lutein Production and mentioning

confidence: 92%

Section: Microalgae Cultivation For Commercial Lutein Production and mentioning

confidence: 99%

Marine Microalgae for Potential Lutein Production

2020

View full text Add to dashboard Cite

show abstract

“…Unsaturated fatty acid content increases to maintain membrane fluidity, while saturated fatty acid content decreases under low temperatures [73]. In addition, the accumulation of secondary carotenoids such as zeaxanthin increases under relatively high temperatures [74], while primary carotenoids, like lutein, are enhanced under low temperatures [75]. In addition, phycobiliprotein accumulation is improved under relatively low temperatures [76].…”

Section: Temperaturementioning

confidence: 99%

“…A temperature decreasing strategy has been applied to lutein production in Chlamydomonas sp. JSC4 [75]. Similarly, a salinity-shift strategy has been applied to biodiesel production in D. salina KSA-HS022 [104] and lutein production in Chlamydomonas sp.…”

Section: Cultivation Strategies Based On Environmental Conditionsmentioning

confidence: 99%

Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds

Wang

Chua

et al. 2020

Marine Drugs

Self Cite

View full text Add to dashboard Cite

Marine microalgae are regarded as potential feedstock because of their multiple valuable compounds, including lipids, pigments, carbohydrates, and proteins. Some of these compounds exhibit attractive bioactivities, such as carotenoids, ω-3 polyunsaturated fatty acids, polysaccharides, and peptides. However, the production cost of bioactive compounds is quite high, due to the low contents in marine microalgae. Comprehensive utilization of marine microalgae for multiple compounds production instead of the sole product can be an efficient way to increase the economic feasibility of bioactive compounds production and improve the production efficiency. This paper discusses the metabolic network of marine microalgal compounds, and indicates their interaction in biosynthesis pathways. Furthermore, potential applications of co-production of multiple compounds under various cultivation conditions by shifting metabolic flux are discussed, and cultivation strategies based on environmental and/or nutrient conditions are proposed to improve the co-production. Moreover, biorefinery techniques for the integral use of microalgal biomass are summarized. These techniques include the co-extraction of multiple bioactive compounds from marine microalgae by conventional methods, super/subcritical fluids, and ionic liquids, as well as direct utilization and biochemical or thermochemical conversion of microalgal residues. Overall, this review sheds light on the potential of the comprehensive utilization of marine microalgae for improving bioeconomy in practical industrial application.

show abstract

“…Chlamydomonas sp. (X Zhao et al, 2019),. and Scenedesmus almeriensis(Molino et al, 2020).The past few decades have witnessed great advances in heterologous biosynthesis of natural products using engineered microbialcell factories.…”

mentioning

confidence: 99%

Hierarchical dynamic regulation of Saccharomyces cerevisiae for enhanced lutein biosynthesis

Bian

Xue

Chen

et al. 2022

Biotech & Bioengineering

View full text Add to dashboard Cite

Lutein, as a carotenoid with strong antioxidant capacity and an important component of macular pigment in the retina, has wide applications in pharmaceutical, food, feed, and cosmetics industries. Besides extraction from plant and algae, microbial fermentation using engineered cell factories to produce lutein has emerged as a promising route. However, intra-pathway competition between the lycopene cyclases and the conflict between cell growth and production are two major challenges. In our previous study, de novo synthesis of lutein had been achieved in Saccharomyces cerevisiae by dividing the pathway into two stages (δ-carotene formation and conversion) using temperature as the input signal to realize sequential cyclation of lycopene. However, lutein production was limited to microgram level, which is still too low to meet industrial demand. In this study, a dual-signal hierarchical dynamic regulation system was developed and applied to divide lutein biosynthesis into three stages in response to glucose concentration and culture temperature. By placing the genes involved in δ-carotene formation under the glucose-responsive ADH2 promoter and genes involved in the conversion of δ-carotene to lutein under temperature-responsive GAL promoters, the growthproduction conflict and intra-pathway competition were simultaneously resolved.Meanwhile, the rate-limiting lycopene ε-cyclation and carotene hydroxylation reactions were improved by screening for lycopene ε-cyclase with higher activity and fine tuning of the P450 enzymes and their redox partners. Finally, a lutein titer of 19.92 mg/L (4.53 mg/g DCW) was obtained in shake-flask cultures using the engineered yeast strain YLutein-3S-6, which is the highest lutein titer ever reported in heterologous production systems.

show abstract

Strategies related to light quality and temperature to improve lutein production of marine microalga Chlamydomonas sp.

Cited by 36 publications

References 40 publications

Marine Microalgae for Potential Lutein Production

Marine Microalgae for Potential Lutein Production

Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds

Hierarchical dynamic regulation of Saccharomyces cerevisiae for enhanced lutein biosynthesis

Contact Info

Product

Resources

About