Strategies for using nanoprobes to perceive and treat cancer activity: a review

Kang, Byunghoon; Kukreja, Aastha; Song, Daesub; Huh, Yong Min; Haam, Seungjoo

doi:10.1186/s13036-016-0044-1

Cited by 11 publications

(4 citation statements)

References 68 publications

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“…The increased uptake of anti-EGFR– p MBA–MGNs in cells clearly shows that (i) EGFR receptors are overexpressed in MDA-MB-231 cells, as supported by our fluorescence assay in Figure S1, (ii) active targeting of MGNs is enabled likely by both surface-bound receptors and receptor-mediated endocytosis, − and (iii) MGNs without antibodies have a low passive uptake in cells within 16 h of incubation. , Unlike the spindle morphology of the MDA-MB-231 cells, these MCF7 cells have more of a brick-shaped morphology and tend to grow in closer proximity to one another (Figure d). Phase contrast images clearly demonstrated that MGNs are not bound to cells either via active targeting (Figure e) or via passive uptake (Figure f).…”

Section: Results and Discussionmentioning

confidence: 58%

Theranostic Gold Nanoantennas for Simultaneous Multiplexed Raman Imaging of Immunomarkers and Photothermal Therapy

Webb¹,

Ou²,

Faley³

et al. 2017

ACS Omega

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In this study, we demonstrate the theranostic capability of actively targeted, site-specific multibranched gold nanoantennas (MGNs) in triple-negative breast cancer (TNBC) cells in vitro. By utilizing multiplexed surface-enhanced Raman scattering (SERS) imaging, enabled by the narrow peak widths of Raman signatures, we simultaneously targeted immune checkpoint receptor programmed death ligand 1 (PDL1) and the epidermal growth factor receptor (EGFR) overexpressed in TNBC cells. A 1:1 mixture of MGNs functionalized with anti-PDL1 antibodies and Raman tag 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB) and MGNs functionalized with anti-EGFR antibodies and Raman tag para-mercaptobenzoic acid (pMBA) were incubated with the cells. SERS imaging revealed a cellular traffic map of MGN localization by surface binding and receptor-mediated endocytosis, enabling targeted diagnosis of both biomarkers. Furthermore, cells incubated with anti-EGFR–pMBA–MGNs and illuminated with an 808 nm laser for 15 min at 4.7 W/cm2 exhibited photothermal cell death only within the laser spot (indicated by live/dead cell fluorescence assay). Therefore, this study not only provides an optical imaging platform that can track immunomarkers with spatiotemporal control but also demonstrates an externally controlled light-triggered therapeutic approach enabling receptor-specific treatment with biocompatible theranostic nanoprobes.

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Section: Results and Discussionmentioning

confidence: 58%

Theranostic Gold Nanoantennas for Simultaneous Multiplexed Raman Imaging of Immunomarkers and Photothermal Therapy

Webb¹,

Ou²,

Faley³

et al. 2017

ACS Omega

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“…An efficient nano–chemotherapeutic system should improve the therapeutic effects of drugs on diseased tissues while reducing its side effects on healthy tissues and organs. , Considering the differences between normal and tumoral tissues in the lymphatic system, capillary walls of blood vessels, and pH levels, , body-intrinsic stimuli-responsive nanosystems are in the focus of recent studies. Among them, pH-responsive drug carriers have been mainly investigated − since they are able to release the cargo drugs only at the site of the action in the acidic microenvironment of the diseased tissue and in the acidic organelles of cells.…”

Section: Introductionmentioning

confidence: 99%

Intracellular Dynamic Disentangling of Doxorubicin Release from Luminescent Nanogold Carriers by Fluorescence Lifetime Imaging Microscopy (FLIM) under Two-Photon Excitation

Suarasan

Craciun

Licărete

et al. 2019

ACS Appl. Mater. Interfaces

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There is still a lack of available techniques to follow noninvasively the intracellular processes as well to track or disentangle various signals from the therapeutic agents at the site of action in the target cells. We present here the assessment of the intracellular kinetics of doxorubicin (DOX) and gold nanoparticle (AuNP) carriers by mapping simultaneously fluorescence and photoluminescence signals by fluorescence lifetime imaging microscopy under two-photon excitation (TPE-FLIM). The new nano–chemotherapeutic system AuNPs@gelatin–hyd–DOX has been fabricated by DOX loading onto the surface of gelatin-biosynthesized AuNPs (AuNPs@gelatin) through a pH-sensitive hydrazone bond. The successful loading of DOX onto the AuNPs was studied by spectroscopic methods and steady-state fluorescence, and the nanosystem pH-responsive character was validated under simulated biological conditions at different pH values (i.e., pH 4.6, 5.3, and 7.4). Considering that the fluorescence lifetime of DOX molecules at a specific point in the cell is a reliable indicator of the discrimination of the different states of the drug in the internalization path, i.e., released versus loaded, the kinetics of AuNPs@gelatin–hyd–DOX cellular uptake and DOX release was compared to that of free DOX, resulting in two different drug internalization pathways. Finally, cell viability tests were conducted against NIH:OVCAR-3 cell line to prove the efficiency of our chemotherapeutic nanosystem. TPE-FLIM technique could be considered promising for noninvasive, high-resolution imaging of cells with improved capabilities over current one-photon-excited FLIM.

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“…The degradation percentage of Mg-ACP/PAA nanoparticles was 72.4% when soaked in an acidic solution (pH = 5.0) for 1 h, which showed improved degradation efficiency than hybrid organosilica nanoparticles. Our results suggest that pH-sensitive Mg-ACP/PAA nanoparticles can be efficiently degraded by lysosomes, which are good candidates for the delivery of drugs and other therapeutic molecules [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Biomimetic inorganic-organic hybrid nanoparticles from magnesium-substituted amorphous calcium phosphate clusters and polyacrylic acid molecules

Cui

Cong

et al. 2021

Bioactive Materials

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Amorphous calcium phosphate (ACP) has been widely found during bone and tooth biomineralization, but the meta-stability and labile nature limit further biomedical applications. The present study found that the chelation of polyacrylic acid (PAA) molecules with Ca 2+ ions in Mg-ACP clusters (~2.1 ± 0.5 nm) using a biomineralization strategy produced inorganic-organic Mg-ACP/PAA hybrid nanoparticles with better thermal stability. Mg-ACP/PAA hybrid nanoparticles (~24.0 ± 4.8 nm) were pH-responsive and could be efficiently digested under weak acidic conditions (pH 5.0–5.5). The internalization of assembled Mg-ACP/PAA nanoparticles by MC3T3-E1 cells occurred through endocytosis, indicated by laser scanning confocal microscopy and cryo-soft X-ray tomography. Our results showed that cellular lipid membranes remained intact without pore formation after Mg-ACP/PAA particle penetration. The assembled Mg-ACP/PAA particles could be digested in cell lysosomes within 24 h under weak acidic conditions, thereby indicating the potential to efficiently deliver encapsulated functional molecules. Both the in vitro and in vivo results preliminarily demonstrated good biosafety of the inorganic-organic Mg-ACP/PAA hybrid nanoparticles, which may have potential for biomedical applications.

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Strategies for using nanoprobes to perceive and treat cancer activity: a review

Cited by 11 publications

References 68 publications

Theranostic Gold Nanoantennas for Simultaneous Multiplexed Raman Imaging of Immunomarkers and Photothermal Therapy

Theranostic Gold Nanoantennas for Simultaneous Multiplexed Raman Imaging of Immunomarkers and Photothermal Therapy

Intracellular Dynamic Disentangling of Doxorubicin Release from Luminescent Nanogold Carriers by Fluorescence Lifetime Imaging Microscopy (FLIM) under Two-Photon Excitation

Biomimetic inorganic-organic hybrid nanoparticles from magnesium-substituted amorphous calcium phosphate clusters and polyacrylic acid molecules

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