Strategies for Tag‐Based Protein Control

Nabet, Behnam; Gray, Nathanael S.; Ferguson, Fleur M.

doi:10.1002/9781119774198.ch13

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2024

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“…We then provide an overview of the challenges and opportunities with the successful application of degron tagging, focusing on those with greatest relevance to in vivo experiments. For a more general overview of protein degradation-based experimental tools, the reader is referred to other recent articles ( Chen et al, 2023 ; Clift et al, 2018 ; Nabet et al, 2022 ; Wu et al, 2020a ).…”

Section: Introductionmentioning

confidence: 99%

Degron tagging for rapid protein degradation in mice

Hernández-Morán,

Taylor,

Lorente-Macías

et al. 2024

Disease Models &Amp; Mechanisms

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Degron tagging allows proteins of interest to be rapidly degraded, in a reversible and tuneable manner, in response to a chemical stimulus. This provides numerous opportunities for understanding disease mechanisms, modelling therapeutic interventions and constructing synthetic gene networks. In recent years, many laboratories have applied degron tagging successfully in cultured mammalian cells, spurred by rapid advances in the fields of genome editing and targeted protein degradation. In this At a Glance article, we focus on recent efforts to apply degron tagging in mouse models, discussing the distinct set of challenges and opportunities posed by the in vivo environment.

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