Strategies for enriching erucic acid from Crambe abyssinica oil by improved Candida antarctica lipase A variants

Zorn, Katja; Oroz‐Guinea, Isabel; Bornscheuer, Uwe T.

doi:10.1016/j.procbio.2018.12.022

Cited by 22 publications

(35 citation statements)

References 30 publications

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“…The following assays were performed as previously described: (1) sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE), (2) protein concentration quantification, (3) determination of the melting temperature, and (4) selection of the best P. pastoris clones (Oroz‐Guinea et al, ; Zorn et al, , b).…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, 5–10 μg were used to transform P. pastoris GS115‐competent cells by electroporation as described by Invitrogen. From each transformation, 20 colonies were picked, and the best P. pastoris clones were selected by screening the Zeocin resistance and the hydrolytic activity of the secreted lipases on indicator plates as previously described (Kouker and Jaeger, ; Zorn et al, , b).…”

Section: Methodsmentioning

confidence: 99%

“…Consequently, several studies analyzing the selectivity of lipases toward different FA and their potential use for the enrichment of gondoic and erucic acids were described. Recently, the creation of Candida antarctica lipase A (CAL‐A) variants was reported by our group as being able to enrich long‐chain mono‐unsaturated FA (MUFA) by either (1) enhanced selectivity toward the hydrolysis of FA shorter than 20 carbon atoms (Zorn et al, , b) or (2) increased tendency for their hydrolysis (Oroz‐Guinea et al, ). Thus, these approaches led to the enrichment of the longer FA in the esterified FA (EFA) fraction or in the FFA fraction, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…For this purpose, the suitability of both lipase isoforms to access enriched FA fractions was investigated in hydrolysis and ethanolysis reactions. In addition, as it was previously observed that the lipase selectivity can be affected by the type of ester substrate used (Oroz‐Guinea et al, ; Zorn et al, , b), during the hydrolysis reactions, two formulations were used, the corresponding TAG and the FAEE derivatives.…”

Section: Introductionmentioning

confidence: 99%

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Enrichment of Erucic and Gondoic Fatty Acids from Crambe and Camelina Oils Catalyzed by Geotrichum candidum Lipases I and II

Oroz‐Guinea

Zorn²,

Bornscheuer³

2019

J Americ Oil Chem Soc

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Erucic (22:1, cisΔ13) and gondoic acids (20:1, cisΔ11) are building blocks obtained from renewable sources for the oleochemical industry. Different biocatalytic strategies for the enrichment of these compounds with high recovery yields were developed in our group. Geotrichum candidum lipases (GCL) strongly discriminate against fatty acids longer than 18 carbon atoms. Thus, GCL-I and -II were investigated using hydrolysis or ethanolysis reactions with Crambe and Camelina oils. Hydrolysis was also studied using fatty acid ethyl esters (FAEE) derived from the corresponding oil. Both isoforms were highly selective; however, interesting differences were observed. Although it has been reported that GCL-I displays a higher preference toward 18 cisΔ9, which is present in the studied oils at high levels, GCL-II showed higher enrichment values during hydrolysis independent of the substrate used. Hence, enrichments of 87% (Crambe oil) and 82% (Crambe FAEE) for erucic acid and 50% (Camelina oil) and 45% (Camelina FAEE) for gondoic acid, with recovery values between 89% and 99%, were achieved. On the contrary, the best enzyme for ethanolysis was GCL-I (82% and 41% for erucic and gondoic acid, respectively). In this case, although GCL-II also displayed good enrichment and recovery levels (77% and 28%, respectively), they were lower compared to the former reactions. In both ethanolysis reactions, the FAEE fraction contained between 92% and 97% of 18 unsaturated fatty acids.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enrichment of Erucic and Gondoic Fatty Acids from Crambe and Camelina Oils Catalyzed by Geotrichum candidum Lipases I and II

Oroz‐Guinea

Zorn²,

Bornscheuer³

2019

J Americ Oil Chem Soc

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“…(Lovatto et al, 2017). Also, the utilization of oil produced by C. abissinica is favored over high erucic acid rapeseed oil due to its higher natural content of C22:1 (Zorn et al, 2019). According to Stankevych (2017), plants of this species are high-yield cultures, which may compete with other oil cultures of Brassicaceae by a complex of parameters.…”

Section: Introductionmentioning

confidence: 99%

Accumulation of Nutrients in the Raw Of Crambe L. Species

Vergun

Shymanska²,

Rakhmetov³

et al. 2019

Agrobiodiversity for Improving of Nutrition, Health and Life Quality

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Investigation of accumulation of different compounds in above-ground part of these plants an important aspect for evaluation of perspective of use. The aim of this study was to compare the peculiarities of the biochemical composition of Crambe species dynamically. Plant material collected from the experimental collection of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. It was studied above-ground parts of C. cordifolia Steven, C. koktebelica (Junge) N. Busch, C. maritima L., C. steveniana Rupr. At the spring vegetation, budding stage, flowering, and fruitage. Following biochemical parameters was studied: dry matter by drying to consist weight at the 105 °C; content of sugars by Bertrand's method using of glucose scale; ascorbic acids with 2.6-dichlorophenolindophenol, tannins with indigo carmine discoloration, organic acids by sodium hydroxide titration with phenolphthalein; carotene with gasoline galosh spectrophotometrically; ash in muffle over. The dry matter during vegetation was from 9.76 (C. cordifolia, budding) to 22.54 (C. maritima at the fruitage) %, total content of sugars from 6.54 (C. maritime at the fruitage) to 33.18 (C. cordifolia at the budding) %, ascorbic acid from 139.85 (C. maritima at the spring vegetation) to 987.02 (C. maritime at the budding) mg%, b-carotene from 0.39 (C. maritima at the budding) to 1.82 (C. cordifolia at the fruitage) mg%, tannins from 1.28 (C. cordifolia at the fruitage) to 7.47 (C. steveniana at the budding) %, content of organic acids from 3.12 (C. steveniana at the fruitage) to 6.28 (C. koktebelica at the spring vegetation) %, ash from 6.11 (C. maritima at the spring vegetation) to 14.61 (C. cordifolia at the flowering) %. Correlation analysis showed that between the content of tannins and organic acids existed positive moderate correlation (r = 0.52). Between other parameters found the weak, very weak positive or negative correlation. Thus, comparing biochemical analyze identified that raw of four species of Crambe is a valuable source of nutrients during vegetation. The high content of sugars, ascorbic acid, and carotene can recommend these plants as forage crops. Also, raw of these plants can be recommended for farther pharmacological investigations.

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Fettsäuren und Fettsäurederivate als nachwachsende Plattformmoleküle für die chemische Industrie

et al. 2021

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Strategies for enriching erucic acid from Crambe abyssinica oil by improved Candida antarctica lipase A variants

Cited by 22 publications

References 30 publications

Enrichment of Erucic and Gondoic Fatty Acids from Crambe and Camelina Oils Catalyzed by Geotrichum candidum Lipases I and II

Enrichment of Erucic and Gondoic Fatty Acids from Crambe and Camelina Oils Catalyzed by Geotrichum candidum Lipases I and II

Accumulation of Nutrients in the Raw Of Crambe L. Species

Fettsäuren und Fettsäurederivate als nachwachsende Plattformmoleküle für die chemische Industrie

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