Strategies for Biofilm Inhibition and Virulence Attenuation of Foodborne Pathogen-Escherichia coli O157:H7

Oloketuyi, Sandra Folarin; Khan, F. Nawaz

doi:10.1007/s00284-017-1314-y

Cited by 36 publications

(17 citation statements)

References 141 publications

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“…Biofilm formation by most pathogenic bacteria results in the survival of the cells under adverse environmental conditions as well as counter effects the host immunological responses. Several strategies have been developed to inhibit biofilm formation as well as to eradicate established mature biofilms [20,60,61]. However, several reports have shown a failure in the treatment of biofilm-associated infections of pathogenic bacteria [62].…”

Section: Anti-persister Activity Of Ua-cs Nps Towards the Biofilm-assmentioning

confidence: 99%

Bactericidal Activity of Usnic Acid-Chitosan Nanoparticles against Persister Cells of Biofilm-Forming Pathogenic Bacteria

Khan

Kim

2020

Marine Drugs

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The present study aimed to prepare usnic acid (UA)-loaded chitosan (CS) nanoparticles (UA-CS NPs) and evaluate its antibacterial activity against biofilm-forming pathogenic bacteria. UA-CS NPs were prepared through simple ionic gelification of UA with CS, and further characterized using Fourier transform infrared spectroscopy, X-ray diffraction, and field-emission transmission electron microscopy. The UA-CS NPs presented a loading capacity (LC) of 5.2%, encapsulation efficiency (EE) of 24%, and a spherical shape and rough surface. The maximum release of UA was higher in pH 1.2 buffer solution as compared to that in pH 6.8 and 7.4 buffer solution. The average size and zeta potential of the UA-CS NPs was 311.5 ± 49.9 nm in diameter and +27.3 ± 0.8 mV, respectively. The newly prepared UA-CS NPs exhibited antibacterial activity against persister cells obtained from the stationary phase in batch culture, mature biofilms, and antibiotic-induced gram-positive and gram-negative pathogenic bacteria. Exposure of sub-inhibitory concentrations of UA-CS NPs to the bacterial cells resulted in a change in morphology. The present study suggests an alternative method for the application of UA into nanoparticles. Furthermore, the anti-persister activity of UA-CS NPs may be another possible strategy for the treatment of infections caused by biofilm-forming pathogenic bacteria.

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Section: Anti-persister Activity Of Ua-cs Nps Towards the Biofilm-assmentioning

confidence: 99%

Bactericidal Activity of Usnic Acid-Chitosan Nanoparticles against Persister Cells of Biofilm-Forming Pathogenic Bacteria

Khan

Kim

2020

Marine Drugs

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“…However, the high expense of this process remains a major obstacle. In addition, a number of food additives, such as nitrite (Steffen, Christiane, Birkenstock, Florian, & Friedrich, 2007), citric acid (Akbas & Kokumer, 2015), and cinnamic aldehyde (Oloketuyi & Khan, 2017b), have been found to exhibit antimicrobial effects against both gram-positive and gram-negative bacteria. Hence, the utilization of physical or chemical methods can negatively affect food quality and cause threats to human health as well as form biofilms on surfaces in contact with food.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of two types of food additives on biofilm formation by foodborne pathogens

Liu

Soteyome

et al. 2019

MicrobiologyOpen

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The inhibition of microbial biofilms is a significant concern in food safety. In the present study, the inhibitory effect of sodium citrate and cinnamic aldehyde on biofilm formation at minimum inhibitory concentrations (MICs) and sub‐MICs was investigated for Escherichia coli O157:H7 and Staphylococcus aureus. The biofilm inhibition rate was measured to evaluate the effect of sodium citrate on S. aureus biofilms at 24, 48, 72, and 96 hr. According to the results, an antibiofilm effect was shown by both food additives, with 10 mg/ml of sodium citrate exhibiting the greatest inhibition of S. aureus biofilms at 24 hr (inhibition rate as high as 77.51%). These findings strongly suggest that sodium citrate exhibits a pronounced inhibitory effect on biofilm formation with great potential in the extension of food preservation and storage.

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“…Since cells within biofilms have low sensitivity to many antimicrobial compounds (7), prevention of biofilm formation is important in disease treatment. A number of different alternative approaches are currently explored as the antibiofilm strategies, including surface modification to prevent bacterial adhesion or assembly of the biofilm matrix, specific enzymes to degrade biofilm matrix, and inhibition of bacterial signaling or quorum sensing using small molecules (8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Multiple Drug-Induced Stress Responses Inhibit Formation of Escherichia coli Biofilms

Teteneva

Mart’yanov

Esteban-López

et al. 2020

Appl Environ Microbiol

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In most ecosystems, bacteria primarily exist as structured surface-associated biofilms that can be highly tolerant to antibiotics and thus represent an important health issue. Here we explored drug repurposing as a strategy to identify new antibiofilm compounds, screening over 1000 compounds from the Prestwick Chemical Library of approved drugs for specific activities that prevent biofilm formation by Escherichia coli. Most growth-inhibiting compounds, which include known antibacterial but also antiviral and other drugs, had also reduced biofilm formation. However, we also identified several drugs that were biofilm-inhibitory at doses where only weak or no effect on planktonic growth could be observed. Activities of the most specific antibiofilm compounds were further characterized using gene expression analysis, proteomics and microscopy. We observed that most of these drugs acted by repressing genes responsible for production of curli, major component of E. coli biofilm matrix. This repression apparently occurred through induction of several different stress responses, including DNA and cell-wall damage, and homeostasis of divalent cations, demonstrating that biofilm formation can be inhibited through a variety of molecular mechanisms. One tested drug, tyloxapol, inhibited biofilm formation independent of curli expression or growth, by suppressing bacterial attachment at the surface. Importance Prevention of bacterial biofilm formation is one of the major current challenges in microbiology. By systematically screening a large number of approved drugs for their ability to suppress biofilm formation by Escherichia coli, here we identified a number of prospective antibiofilm compounds. We further demonstrated different mechanisms of action for individual compounds, from induction of replicative stress to disbalance of cation homeostasis to inhibition of bacterial attachment to the surface. Our work demonstrates the potential of drug repurposing for the prevention of bacterial biofilm formation and suggests that also for other bacteria the activity spectrum of antibiofilm compounds is likely to be broad.

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Strategies for Biofilm Inhibition and Virulence Attenuation of Foodborne Pathogen-Escherichia coli O157:H7

Cited by 36 publications

References 141 publications

Bactericidal Activity of Usnic Acid-Chitosan Nanoparticles against Persister Cells of Biofilm-Forming Pathogenic Bacteria

Bactericidal Activity of Usnic Acid-Chitosan Nanoparticles against Persister Cells of Biofilm-Forming Pathogenic Bacteria

Inhibitory effects of two types of food additives on biofilm formation by foodborne pathogens

Multiple Drug-Induced Stress Responses Inhibit Formation of Escherichia coli Biofilms

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