Strategies and perspectives to develop SARS-CoV-2 detection methods and diagnostics

Jalandra, Rekha; Yadav, Amit K.; Verma, Deepali; Dalal, Nishu; Sharma, Minakshi; Singh, Rajeev; Kumar, Anil; Solanki, Pratima R.

doi:10.1016/j.biopha.2020.110446

Cited by 82 publications

(60 citation statements)

References 60 publications

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“…1- RNA isolation followed by cDNA synthesis with reverse transcription kit. 2- Mixing buffer, DNA polymerase enzyme, primers of a target gene, deoxynucleoside triphosphate, cDNA template and fluorescent dye, 3- Incubation of the mixture at different temperatures to perform thermal cycling in PCR instrument and fluorescence measurements for calculating Cycle threshold (Ct) data, and 4- Relative expression estimation based on Ct data of control and experimental samples [ 23 ]. The selection of a standard positive control influences the accuracy of RT-PCR [ 47 ].…”

Section: Current Detection Methods For Sars-cov-2mentioning

confidence: 99%

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Diagnostic techniques for COVID-19 and new developments

Sheikhzadeh

Eissa

Ismail

et al. 2020

Talanta

126

129

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COVID-19 pandemic is a serious global health issue today due to the rapid human to human transmission of SARS-CoV-2, a new type of coronavirus that causes fatal pneumonia. SARS -CoV-2 has a faster rate of transmission than other coronaviruses such as SARS and MERS and until now there are no approved specific drugs or vaccines for treatment. Thus, early diagnosis is crucial to prevent the extensive spread of the disease. The reverse transcription-polymerase chain reaction (RT-PCR) is the most routinely used method until now to detect SARS-CoV-2 infections. However, several other faster and accurate assays are being developed for the diagnosis of COVID-19 aiming to control the spread of infection through the identification of patients and immediate isolation. In this review, we will discuss the various detection methods of the SARS-CoV-2 virus including the recent developments in immunological assays, amplification techniques as well as biosensors.

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Section: Current Detection Methods For Sars-cov-2mentioning

confidence: 99%

“…Moreover, mutations lead to faster transport of viruses from animals to humans and humans to humans. Mutations in the ORF8 region at 28,144 and the ORF1B region at 8872 were reported in the early phase of the SRAS-CoV-2 epidemic [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic techniques for COVID-19 and new developments

Sheikhzadeh

Eissa

Ismail

et al. 2020

Talanta

126

129

View full text Add to dashboard Cite

show abstract

“…Due to the phylogenetic connection and similarity in the genome sequence, this novel species of CoV, SARS-CoV-2, has been placed under the β CoV genus of the CoV family. It has a positive sense of large 30 kb, single-stranded RNA (ssRNA) genome, which is spherical [ 18 , 21 ]. As the genetic material of this virus is RNA, its two-third part consists of non-structural polyproteins (ORF1a-ORF1b), i.e., RNA synthesis materials, and encodes viral polymerase RNA dependent RNA polymerase (RdRp).…”

Section: Sars-cov-2: Physical Characteristics Structure and Genomicmentioning

confidence: 99%

The perspectives of biomarker-based electrochemical immunosensors, artificial intelligence and the Internet of Medical Things toward COVID-19 diagnosis and management

Yadav

Verma

Kumar

et al. 2021

Materials Today Chemistry

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The WHO has declared the COVID-19 an international health emergency due to the severity of infection progression which become more severe due to its continuous spread globally and the unavailability of appropriate therapy and diagnostics systems. Thus, there is a need for efficient devices to detect SARS-CoV-2 infection at an early stage. Nowadays, the RT-PCR technique is being applied for detecting this virus around the globe; however, factors such as stringent expertise, long diagnostic times, invasive and painful screening, and high costs have restricted the use of RT-PCR methods for rapid diagnostics. Therefore, the development of cost-effective, portable, sensitive, prompt, and selective sensing systems to detect SARS-CoV-2 in biofluids at fM/pM/nM concentrations would be a breakthrough in diagnostics. Immunosensors that show increased specificity and sensitivity are considerably fast, and don’t imply costly reagents or instruments, reducing the cost for COVID-19 detection. The current developments in immunosensors perhaps signify the most significant opportunity for a rapid assay to detect COVID-19, without the need of highly skilled professionals and specialized tools to interpret results. Artificial intelligence (AI) and the Internet of Medical Things (IoMT) can also be equipped with this immunosensing approach to investigate useful networking through database management, sharing, and analytics to prevent and manage COVID-19. Herein, we represent the collective concepts of biomarkers based immunosensors along with AI and IoMT as smart sensing strategies with bioinformatics approach to monitor non-invasive early stage SARS-CoV-2 development, with fast POC diagnostics as the crucial goal. This approach should be implemented quickly and verified practicality for clinical samples before being set in the present times for mass-diagnostic research.

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“…In this regard, both developing and developed nations are continuously working out to find a suitable solution for this pandemic. Among them, most of the work is done in detecting and curing COVID‐19 and manufacturing different testing and diagnosis kits, development of vaccine, antiviral drugs, and other medical facilities 7–10. On the other side, research is also going on the physical protection from COVID‐19 by using various masks and respirators.…”

Section: Introductionmentioning

confidence: 99%

Graphene‐based Materials for Fighting Coronavirus Disease 2019: Challenges and Opportunities

et al. 2021

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The coronavirus disease 2019 (COVID‐19) caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is considered as serious global threat of this time and greatest challenge for recent days. Several approaches have been carried out in this direction to fight against COVID‐19. Among these, nanotechnology is one of the promising approach to face these challenges in the current situation. Recently, graphene‐based nanomaterials have been explored for COVID‐19 due to its unique physicochemical properties. This mini review provides a recent progress in graphene‐based nanomaterials and its applications for diagnosis, detection, decontamination, and protection against COVID‐19. Further, main challenges and perspective for fundamental design and development of technologies based on graphene‐based materials are discussed and suitable directions to improve these technologies are suggested. This article will provide timely knowledge and future direction about this wonder materials in various biological applications.

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Strategies and perspectives to develop SARS-CoV-2 detection methods and diagnostics

Cited by 82 publications

References 60 publications

Diagnostic techniques for COVID-19 and new developments

Diagnostic techniques for COVID-19 and new developments

The perspectives of biomarker-based electrochemical immunosensors, artificial intelligence and the Internet of Medical Things toward COVID-19 diagnosis and management

Graphene‐based Materials for Fighting Coronavirus Disease 2019: Challenges and Opportunities

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