Strategic growth of limnic green microalgae with phycoremediation potential for enhanced production of biomass and biomolecules for sustainable environment

Sureshkumar, Pandian; Thomas, Jibu

doi:10.1007/s11356-018-4012-9

Cited by 9 publications

(2 citation statements)

References 24 publications

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“…Compared to photoautotrophy, heterotrophic cultivation can significantly increase growth rate and biomass productivity, eliminate the light dependence, and be easier to control monoculture and scale up [ 2 ]. Due to these advantages, hetertrophic production of valuable products, including pigments, fatty acids, pharmaceuticals, and biofuels, has received substantially increasing interest [ 3 – 5 ]. Under heterotrophic culture, many microalgae, such as Haematococcus pluvialis , Chlorella protothecoides , Galdieria sulphuraria , Nitzschia laevis , Crypthecodinium cohnii, and Neochloris oleoabundans , have been reported to have the potential to accumulate high biomass, fatty acids, or large quantities of valuable chemicals [ 4 , 6 – 10 ].…”

Section: Introductionmentioning

confidence: 99%

Biomass, lipid accumulation kinetics, and the transcriptome of heterotrophic oleaginous microalga Tetradesmus bernardii under different carbon and nitrogen sources

Gao

Wang

Huang

et al. 2021

Biotechnol Biofuels

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Background Heterotrophic cultivation of microalgae has been proposed as a viable alternative method for novel high-value biomolecules, enriched biomass, and biofuel production because of their allowance of high cell density levels, as well as simple production technology. Tetradesmus bernardii, a newly isolated high-yielding oleaginous microalga under photoautotrophic conditions, is able to grow heterotrophically, meaning that it can consume organic carbon sources in dark condition. We investigated the effect of different carbon/nitrogen (C/N) ratios on the growth and lipid accumulation of T. bernardii in heterotrophic batch culture under two nitrogen sources (NaNO3 and CO(NH2)2). In addition, we conducted time-resolved transcriptome analysis to reveal the metabolic mechanism of T. bernardii in heterotrophic culture. Results T. bernardii can accumulate high biomass concentrations in heterotrophic batch culture where the highest biomass of 46.09 g/L was achieved at 100 g/L glucose concentration. The rate of glucose to biomass exceeded 55% when the glucose concentration was less than 80 g/L, and the C/N ratio was 44 at urea treatment. The culture was beneficial to lipid accumulation at a C/N ratio between 110 and 130. NaNO3 used as a nitrogen source enhanced the lipid content more than urea, and the highest lipid content was 45% of dry weight. We performed RNA-seq to analyze the time-resolved transcriptome of T. bernardii. As the nitrogen was consumed in the medium, nitrogen metabolism-related genes were significantly up-regulated to speed up the N metabolic cycle. As chloroplasts were destroyed in the dark, the metabolism of cells was transferred from chloroplasts to cytoplasm. However, storage of carbohydrate in chloroplast remained active, mainly the synthesis of starch, and the precursor of starch synthesis in heterotrophic culture may largely come from the absorption of organic carbon source (glucose). With regard to lipid metabolism, the related genes of fatty acid synthesis in low nitrogen concentration increased gradually with the extension of cultivation time. Conclusion T. bernardii exhibited rapid growth and high lipid accumulation in heterotrophic culture. It may be a potential candidate for biomass and biofuel production. Transcriptome analysis showed that multilevel regulation ensured the conversion from carbon to the synthesis of carbohydrate and lipid.

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Section: Introductionmentioning

confidence: 99%

Biomass, lipid accumulation kinetics, and the transcriptome of heterotrophic oleaginous microalga Tetradesmus bernardii under different carbon and nitrogen sources

Gao

Wang

Huang

et al. 2021

Biotechnol Biofuels

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“…In particular, N starvation triggered lipid accumulation, especially TAGs, in microalgae such as Scenedesmus sp. [40][41][42][43][44]. In general, an inverse relationship has been reported between lipid and nutrient concentrations [43].…”

Section: Nutrient Consumption Of S Rubescens Knua042mentioning

confidence: 99%

Nitrogen Deficiency-Dependent Abiotic Stress Enhances Carotenoid Production in Indigenous Green Microalga Scenedesmus rubescens KNUA042, for Use as a Potential Resource of High Value Products

Hong

et al. 2020

Sustainability

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The microalgal strain Scenedesmus rubescens KNUA042 was identified in freshwater in Korea and characterized by evaluating its stress responses in an effort to increase lipid and carotenoid production. Under a two-stage cultivation process, the algal strain that generally exhibits optimal growth at a nitrate (source of nitrogen) concentration of 0.25 g L−1 was challenged to different exogenous stimuli—salinity (S), light intensity (L), combined L and S (LS), and nitrogen deficiency (C)—for 14 days. Lipid production and carotenoid concentration increased in a time-dependent manner under these physicochemical conditions during the culture periods. Lipid accumulation was confirmed by thin layer chromatography, BODIPY staining, and fatty acid composition analysis, which showed no differences in the algal cells tested under all four (C, S, L, and LS) conditions. The quality of biodiesel produced from the biomass of the algal cells met the American Society for Testing and Materials and the European standards. Total carotenoid content was increased in the LS-treated algal cells (6.94 mg L−1) compared with that in the C-, S-, and L-treated algal cells 1.75, 4.15, and 1.32 mg L−1, respectively). Accordingly, the concentration of canthaxanthin and astaxanthin was also maximized in the LS-treated algal cells at 1.73 and 1.11 mg g−1, respectively, whereas lutein showed no differences in the cells analyzed. Conversely, chlorophyll a level was similar among the C-, S-, and LS-treated algal cells, except for the L-treated algal cells. Thus, our results suggested that S. rubescens KNUA042 was capable of producing carotenoid molecules, which led to the maximum values of canthaxanthin and astaxanthin concentrations when exposed to the combined LS condition compared with that observed when exposed to the salinity condition alone. This indicates that the algal strain could be used for the production of high-value products as well as biofuel. Furthermore, this article provides the first evidence of carotenoid production in S. rubescens KNUA042.

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Microalgae-based domestic wastewater treatment: a review of biological aspects, bioremediation potential, and biomass production with biotechnological high-value

de Cassia Soares Brandão,

Oliveira,

dos Santos

et al. 2023

Environ Monit Assess

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Strategic growth of limnic green microalgae with phycoremediation potential for enhanced production of biomass and biomolecules for sustainable environment

Cited by 9 publications

References 24 publications

Biomass, lipid accumulation kinetics, and the transcriptome of heterotrophic oleaginous microalga Tetradesmus bernardii under different carbon and nitrogen sources

Biomass, lipid accumulation kinetics, and the transcriptome of heterotrophic oleaginous microalga Tetradesmus bernardii under different carbon and nitrogen sources

Nitrogen Deficiency-Dependent Abiotic Stress Enhances Carotenoid Production in Indigenous Green Microalga Scenedesmus rubescens KNUA042, for Use as a Potential Resource of High Value Products

Microalgae-based domestic wastewater treatment: a review of biological aspects, bioremediation potential, and biomass production with biotechnological high-value

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