Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in<i>Cunninghamella bainieri</i>2A1

Shuib, Salwati; Nawi, Wan Nazatul Naziah Wan; Taha, Ekhlass M.; Omar, Othman; Kader, Abdul; Kalil, Mohd Sahaid; Hamid, Aidil Abdul

doi:10.1155/2014/173574

Cited by 7 publications

(7 citation statements)

References 32 publications

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“…Effect of metal ions on lipid production by fungi Cunninghamella bainieri has been investigated (Shuib et al, 2014). The fungus produced up to 8.42 g/L biomass with 32% lipid content during nitrogen limitation.…”

Section: Metals and Non-metalsmentioning

confidence: 99%

A review on variation in crude glycerol composition, bio-valorization of crude and purified glycerol as carbon source for lipid production

Kumar

Yellapu

Tyagi

et al. 2019

Bioresource Technology

145

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Crude glycerol (CG) is a by-product formed during the trans-esterification reaction for biodiesel production. Although crude glycerol is considered a waste stream of the biodiesel industry, it can replace expensive carbon substrates required for lipid production by oleaginous micro-organisms.However, crude glycerol has several impurities, such as methanol, soap, triglycerides, fatty acids, salts and metals, which are created during the trans-esterification process and may affect the cellular metabolism involved in lipid synthesis. This review aims to critically present a variation in crude glycerol composition depending on trans-esterification process and impact of impurities present in the crude glycerol on the cell growth and lipid accumulation by oleaginous microbes.This study also draws comparison between purified and crude glycerol for lipid production. Several techniques for crude glycerol purification (chemical treatment, thermal treatment, membrane technology, ion-exchange chromatography and adsorption) have been presented and discussed with reference to cost and environmental effects.

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Section: Metals and Non-metalsmentioning

confidence: 99%

A review on variation in crude glycerol composition, bio-valorization of crude and purified glycerol as carbon source for lipid production

Kumar

Yellapu

Tyagi

et al. 2019

Bioresource Technology

145

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“…It is well known that C. bainieri 2A1 is capable of producing up to 30% lipid (g/g biomass) which contains 10–15% GLA. In this regard, nutritional intake of GLA and other PUFAs have been used in clinical treatment of human diseases such as blood cholesterol, acute and chronic inflammations, and atopic eczema, hypertension, Crohn’s disease, rheumatoid arthritis and asthma (Shuib et al, 2014, Stoll, 2002, Vadivelan and Venkateswaran, 2014).…”

Section: Introductionmentioning

confidence: 99%

Batch culture and repeated-batch culture of Cunninghamella bainieri 2A1 for lipid production as a comparative study

Dashti

Abdeshahian

2016

Saudi Journal of Biological Sciences

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This research was performed based on a comparative study on fungal lipid production by a locally isolated strain Cunninghamella bainieri 2A1 in batch culture and repeated-batch culture using a nitrogen-limited medium. Lipid production in the batch culture was conducted to study the effect of different agitation rates on the simultaneous consumption of ammonium tartrate and glucose sources. Lipid production in the repeated-batch culture was studied by considering the effect of harvesting time and harvesting volume of the culture broth on the lipid accumulation. The batch cultivation was carried out in a 500 ml Erlenmeyer flask containing 200 ml of the fresh nitrogen-limited medium. Microbial culture was incubated at 30 °C under different agitation rates of 120, 180 and 250 rpm for 120 h. The repeated-batch culture was performed at three harvesting times of 12, 24 and 48 h using four harvesting cultures of 60%, 70%, 80% and 90%. Experimental results revealed that nitrogen source (ammonium tartrate) was fully utilized by C. bainieri 2A1 within 24 h in all agitation rates tested. It was also observed that a high amount of glucose in culture medium was consumed by C. bainieri 2A1 at 250 rpm agitation speed during the batch fermentation. Similar results showed that the highest lipid concentration of 2.96 g/L was obtained at an agitation rate of 250 rpm at 120 h cultivation time with the maximum lipid productivity of 7.0 × 10−2 mg/ml/h. On the other hand, experimental results showed that the highest lipid concentration produced in the repeated-batch culture was 3.30 g/L at the first cycle of 48 h harvesting time using 70% harvesting volume, while 0.23 g/L gamma-linolenic acid (GLA) was produced at the last cycle of 48 h harvesting time using 80% harvesting volume.

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“…Mg, K, Na, Fe, Zn, Co). It is also affected by limited nitrogen concentration that inhibits the activation of the NAD isocitrate dehydrogenase resulting in accumulation of citrate and increased acetyl-CoA and lipid synthesis (Tsakona et al, 2014;Shuib et al, 2014;Calvey at al., 2016). Therefore, the results of this study indicate that the used AIW contained sufficient metal and nitrogen sources for lipid accumulation in L. starkeyi.…”

Section: Discussionmentioning

confidence: 99%

Porous cellulose as promoter of oil production by the oleaginous yeast Lipomyces starkeyi using mixed agroindustrial wastes

Ganatsios

Κουτίνας

Bekatorou

et al. 2017

Bioresource Technology

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Enhanced single cell oil (SCO) production by the oleaginous yeast Lipomyces starkeyi DSM 70296, immobilised on delignified porous cellulose, is reported. Pure glucose media were initially used. The effects of substrate pH and treatment temperature were evaluated, showing that 30°C and pH 5.0 were the optimum conditions for SCO production by the immobilised yeast. The immobilisation technique led to increased lipid accumulation and cell growth by 44% and 8%, respectively, in the glucose media, compared to free cells in suspension. This positive effect was also shown when low concentration mixed agro-industrial waste suspensions were used as substrates, leading to 85% enhanced SCO production in comparison with free cells. Higher fatty acid (HFA) analysis showed that yeast immobilisation led to increased formation of unsaturated HFAs (6%) and reduced saturated HFAs (5%) compared to free cells.

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Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation inCunninghamella bainieri2A1

Cited by 7 publications

References 32 publications

A review on variation in crude glycerol composition, bio-valorization of crude and purified glycerol as carbon source for lipid production

A review on variation in crude glycerol composition, bio-valorization of crude and purified glycerol as carbon source for lipid production

Batch culture and repeated-batch culture of Cunninghamella bainieri 2A1 for lipid production as a comparative study

Porous cellulose as promoter of oil production by the oleaginous yeast Lipomyces starkeyi using mixed agroindustrial wastes

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