Strains ofaspergillus versicolor tiraboschi synthesizing sterigmatocystin and the differentiation of mycotoxic risk dependent on their productivity in housing buildings

Civil and Environmental Engineering Reports

2018

The intense pace of construction work means that technical materials contain so-called technological moisture, which enables the development of biodeteriogenic organisms on building partitions. This article presents the mycological analysis of two buildings in Zielona Góra, whose building partitions were affected by colour deposits. In the first building (a block of flats in the shell and core condition) 7 species of moulds were determined, of which the dominant one was Cladosporium herbarum. In the second building (public utilities building) changes on the walls appeared just after refurbishment and were caused by a species of mould called Strachybotrys chartarum which is hazardous to human and animal life.

Section: Testing Methodologymentioning

confidence: 99%

Technological Moisture as a Cause of Moulds on Building Partitions

Civil and Environmental Engineering Reports

2018

“…Czapek-Dox and MEA. The time of plating, cultivation and observation for an isolated single species was 21 days [17,18]. The isolated strains were subjected to identification tests using keys for taxonomic identifiers: 19-32, 15.…”

Section: Methodsmentioning

confidence: 99%

Spergilli on Building Partitions Infested with Moulds in Residential Housing and Public Utility Premises

Civil and Environmental Engineering Reports

2017

A b s t r a c tAspergilli constitute a serious risk to the health of the inhabitants of infested rooms. Mycological analysis conducted in buildings infected with moulds in the area of the Lubuskie province (Poland) demonstrated the presence of 9 species of Aspergillus moulds: A. carbonarius A. clavatus, A. flavus, A. fumigatus, A. niger, A. ochraceus, A. terreus, A ustus and A. versicolor. The highest frequency (4 -frequently) was observed in the case of A. versicolor, while frequency 3 (fairly frequently) was characteristic of such species as A. flavus and A. niger. A. ustus was encountered with frequency 2 (individually), while frequency 1 (sporadically) referred to four species: A. carbonarius, A. clavatus, A. fumigatus and A. terreus. Because Aspergillus versicolor occurs with the highest frequency in buildings, and as a consequence of this, synthesizes toxic and carcinogenic sterigmatocystin (ST), it constitutes the greatest risk to the inhabitants of the infested premises. All species of Aspergillus present on building partitions are able to synthesise mycotoxins, are pathogens and may cause allergies.

“…Pure (axenic) cultures were isolated from mixed starter cultures by their passaging on the media: Cz and MEA. The time of plating, cultivation and observation for an isolated single species was 21 days [ 38 , 39 ]. The isolated strains were subjected to identification tests using keys for taxonomic identifiers: [ 12 , 14 , 15 , 19 , 26 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ].…”

Section: Methodsmentioning

confidence: 99%

Occurrence of the Toxin-Producing Aspergillus versicolor Tiraboschi in Residential Buildings

2016

IJERPH

In an area representative of a moderate climate zone (Lubuskie Province in Poland), mycological tests in over 270 flats demonstrated the occurrence of 82 species of moulds. Aspergillus versicolor Tiraboschi was often encountered on building partitions (frequency 4: frequently). The ability to synthesize the carcinogenic sterigmatocystin (ST) means that it poses a risk to humans and animals. Biotoxicological tests of biomasses of A. versicolor were conducted in the Microbiological and Toxicological Laboratory, using the planarians Dugesia tigrina (Girard). The obtained results of the tests covered a broad range of toxicity levels of isolated strains: from weakly toxic (100–1000 mg·L−3) to potently toxic (1–10 mg·L−3). The high-performance liquid chromatography (HPLC) physicochemical method confirmed the ability of A. versicolor strains to synthesize sterigmatocystin. All of the samples of the air-dry biomasses of the fungi contained ST in the range between 0.03 and 534.38 mg·kg−1. In the bio-safety level (BSL) classification A. versicolor belongs to category 1. Additionally, A. versicolor is an allergenic mould.