Strains of Lactobacillus plantarum in Grape Must are also Present in the Intestinal Tract of Vinegar Flies

Abstract

Twenty-one lactic acid bacteria isolated from the intestinal tract of Drosophila simulans Stuvervant and nine from Merlot noir grapes were identified as L. plantarum by PCR with species-specific primers and 16S rDNA sequencing. The 30 isolates grouped into four clusters based on RAPD-PCR banding patterns, suggesting that they belong to at least four genotypic groups. Thirteen isolates from grape must and five from the flies yielded identical RAPD-PCR banding patterns and grouped into one cluster, suggesting that they are descendants from the same strain. Concluded from these results, L. plantarum (or at least descendants from a specific strain) has the ability to use vinegar flies as a host and vector to infect grape must. Further research is needed to determine the role of this specific strain in wine fermentations.

Microbiome-by-ethanol interactions impact Drosophila melanogaster fitness, physiology, and behavior

“…Acetobacters are known ethanol degraders ( Shimizu et al., 1977 ), which might be reflected in their lower tolerance since they decrease ethanol concentrations and prevent them from reaching toxic levels. Lactobacilli are known wine souring organisms ( Groenewald et al., 2006 ), which might be reflected in their ability to tolerate higher ethanol concentrations. These results indicate that the bacterial composition of flies varies, at least in part, according to the ethanol sensitivities of the bacterial strains.…”