Strain Variation among
            <i>Bordetella pertussis</i>
            Isolates from Québec and Alberta Provinces of Canada from 1985 to 1994

Peppler, M S; Kuny, Sharee; Nevesinjac, Anna Z.; Rogers, Christina; Moissac, Yvon R. de; Knowles, Kathleen; Lorange, Manon; Serres, Gaston De; Talbot, James

doi:10.1128/jcm.41.7.3344-3347.2003

Cited by 25 publications

(30 citation statements)

References 21 publications

(20 reference statements)

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“…Recent Dutch isolates were shown to be distinguishable from strains that had been used for the production of vaccines. These same findings were reproduced in many other countries (10,12,14,22,29). Acellular pertussis vaccines were introduced nationwide in Sweden in 1996, 17 years after the withdrawal of whole-cell pertussis vaccine from the childhood immunization schedule (20).…”

supporting

confidence: 54%

“…Based on the profiles obtained with each of these two enzymes, Weber et al (29) identified six main groups of B. pertussis, i.e., groups I, II, III, IV␣, IV␤, and V. Reference strains were recommended for strain characterization at the group level (18,29). Many recent studies have put forward the idea of a one-enzyme system, primarily using XbaI (4,5,7,14,22,24). In many of these studies, references to internationally available type strains were lacking (4,5,7,14).…”

mentioning

confidence: 99%

“…Pulsed-field gel electrophoresis (PFGE) is used as an epidemiological tool for surveillance studies of B. pertussis strain variation over time and for identification of outbreak-associated isolates (4,5,7,14,22,24,29). It is of particular interest to monitor the bacterial population and its possible influence on vaccine effectiveness after the introduction of acellular vaccines.…”

mentioning

confidence: 99%

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Reference System for Characterization of Bordetella pertussis Pulsed-Field Gel Electrophoresis Profiles

2004

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Pulsed-field gel electrophoresis (PFGE) has been used as an epidemiological tool for surveillance studies of Bordetella pertussis since the early 1990s. To date there is no standardized procedure for comparison of results, and therefore it has been difficult to directly compare PFGE results between laboratories. We propose a profile-based reference system for PFGE characterization of B. pertussis strain variation and to establish traceability of B. pertussis PFGE results. We initially suggest 35 Swedish reference strains as reference material for PFGE traceability. This reference material is deposited at the Culture Collection of the University of Gothenburg, Gothenburg, Sweden. Altogether, 1,810 Swedish clinical isolates from between 1970 and 2003 were studied, together with the Swedish Pw vaccine strain, six reference strains, and two U.S. isolates. Our system provides evidence that profiles obtained by using only one enzyme, i.e., XbaI, give enough data to analyze the epidemiological relationship between them. Characterization with one enzyme is far less labor intensive, yielding results in half the time than when a two-enzyme procedure is used. Also, we can see that there is a correlation between PFGE profile and pertactin type. One common PFGE profile, BpSR11 (n ‫؍‬ 455), showed 100% prn2 and 100% Fim3 when analyzed for pertactin type and serotype. On the other hand, strains with the same profile may express various serotypes when isolated over longer periods of time. Subculturing of the same isolate eight times or lyophilization caused no change in PFGE profile.

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confidence: 54%

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confidence: 99%

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Reference System for Characterization of Bordetella pertussis Pulsed-Field Gel Electrophoresis Profiles

2004

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“…One possible explanation for this resurgence was that the circulating B. pertussis population consisted of strains that were antigenically distinct from the vaccine strains (Mooi et al, 1998). Subsequent studies have reported allelic variation in these two markers in Finland, the UK, Poland, the USA and Canada Cassiday et al, 2000;Fry et al, 2001;Gzyl et al, 2002;Peppler et al, 2003). Additional targets under presumed selective pressure were examined for nucleotide sequence variation by , including other genes encoding proteins used as acellular vaccine (ACV) components and other surface-associated proteins.…”

Section: Introductionmentioning

confidence: 99%

Sequence variation and conservation in virulence-related genes of Bordetella pertussis isolates from the UK

Packard

Parton

Coote

et al. 2004

Journal of Medical Microbiology

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To determine the value of gene markers for surveillance and to assess the genetic stability of potential acellular pertussis vaccine components, the sequence variation in ten virulence-related genes of Bordetella pertussis was investigated in strains isolated in the UK between 1920 and 2002. These genes encode: pertactin (prnA); pertussis toxin subunits S1 (ptxA) and S3 (ptxC); tracheal colonization factor (tcfA); bordetella autotransporter protein C (bapC); bordetella resistance to killing protein (brkA); fimbrial antigen 2 (fim2); outer-membrane protein Q (ompQ); virulence-activated gene 8 (vag8) and adenylate cyclase toxin (cyaA). The encoded proteins are either components of current acellular vaccines (ACVs), or potential virulence markers for B. pertussis. Three strains used in the pertussis UK whole-cell vaccine (WCV), strain Tohama-I used for production of ACV components and the type strain of B. pertussis (18323 T ) were also analysed. Several novel alleles were found. The UK isolates were assigned multi-locus sequence types (MLSTs) according to a previously described scheme for B. pertussis based on three of these genes (ptxA, ptxC and tcfA). Compared with isolates from other countries, the UK clinical strains showed a distinct distribution of MLSTs. Apart from one strain that was MLST-3, all other recent isolates (2000)(2001)(2002) were identified as MLST-5. These isolates differed from the three WCV strains, which were MLST-2 or MLST-3, the Tohama-I strain (MLST-2) and the type strain of B. pertussis (MLST-9). MLST-3 and MLST-5 differ only by a single synonymous mutation, but this method does indicate that currently circulating strains of B. pertussis are not identical to the vaccine types, and they may differ in other important characteristics. Two new MLSTs were identified amongst historical UK isolates. Sequence-based typing offers a convenient method of analysing and comparing populations of B. pertussis from different time periods and from different countries. The variation exhibited by prnA and fim2 suggests that they could be useful, additional epidemiological markers in such a typing scheme.

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“…Infants too young to be fully immunized have become the most vulnerable group at highest risk for severe clinical disease, whereas adolescents and adults, previously protected, have become populations susceptible to mild disease (Figure 1) [28,29]. Since the 1990s, a steady increase in the number of pertussis cases was observed in various countries with successful and long-lasting vaccination programs, such as Argentina, Finland, Norway, Spain, Switzerland, Israel, Canada, Australia, the USA and The Netherlands [30][31][32][33][34]. In these countries, different types of pertussis vaccines were being used with proven efficacy in clinical trials.…”

Section: Resurgence Of Pertussismentioning

confidence: 99%

Resurgence of pertussis calls for re-evaluation of pertussis animal models

Aa¹,

Hozbor²,

Cj³

et al. 2012

Expert Review of Vaccines

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Strain Variation among Bordetella pertussis Isolates from Québec and Alberta Provinces of Canada from 1985 to 1994

Cited by 25 publications

References 21 publications

Reference System for Characterization of Bordetella pertussis Pulsed-Field Gel Electrophoresis Profiles

Reference System for Characterization of Bordetella pertussis Pulsed-Field Gel Electrophoresis Profiles

Sequence variation and conservation in virulence-related genes of Bordetella pertussis isolates from the UK

Resurgence of pertussis calls for re-evaluation of pertussis animal models

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