Strain Variation among Bordetella pertussis Isolates Circulating in Poland after 50 Years of Whole-Cell Pertussis Vaccine Use

Abstract

In the present study, clinical isolates of Bordetella pertussis collected in Poland from 1960 to 2005 were analyzed by pulsed-field gel electrophoresis (PFGE) according to protocols recommended in previous studies. Among the 110 isolates from 1995 to 2005, 59 PFGE patterns were found, most of which were different from those currently circulating in other European Union (EU) countries for which data are available. The PFGE patterns of currently disseminating B. pertussis clones were found within PFGE groups III and IV, as elsewhere in the EU, and in newly identified clusters A and C. Up to 70, 26, and 4%, respectively, of the currently isolated strains in Poland harbored ptxA1-prn1, ptxA1-prn2, and ptxA1-prn3 allele combinations, and most (82%) were found to be of the Fim2 phenotype. Differences in the extent of heterogeneity estimated by PFGE typing in B. pertussis populations circulating in Poland in comparison to other EU countries may be due to the different vaccine composition strategy, since routine pertussis vaccination was initiated in Poland in 1960.

Pulsed-Field Gel Electrophoresis Analysis of Bordetella pertussis Isolates Circulating in Europe from 1998 to 2009

“…However, in countries such as Finland, France, The Netherlands, and Sweden, where the serial collection of isolates was done and tested, the frequencies in the major profiles observed were similar to those found in the present study. Moreover, a recent Polish study reported that there were 59 PFGE profiles identified among 110 isolates from 1995 to 2005 (38). Most of these profiles were different from those observed in this study, confirming that the trends/ proportions observed in this study were representative.…”

Sequence variation in virulence-related genes of Bordetella pertussis isolates from Poland in the period 1959–2013

“…Similar to many other countries [11, 13-15, 17, 22], genetic divergence between vaccine strains and clinical isolates in Poland has also been found [23]. Nevertheless, genetic changes observed in B. pertussis isolates circulating in Poland were found to be quite different to those seen elsewhere in Europe [23].…”

“…Nevertheless, genetic changes observed in B. pertussis isolates circulating in Poland were found to be quite different to those seen elsewhere in Europe [23]. Polish B. pertussis isolates from 1999 to 2004 were rarely similar to those observed in other European countries using the PFGE technique [12,13].…”

Serotypes and genetic profiles of <i>Bordetella pertussis</i> strains isolated in the city of São Paulo, 2006-2008

“…Entre as metodologias disponíveis que são capazes de monitorar diferenças genéticas entre isolados, a PFGE foi escolhida como método de referência para estudos epidemiológicos da coqueluche, por apresentar um maior poder discriminatório 7,8 .…”

“…Embora a identificação do sorotipo seja importante para a caracterização primária das cepas, métodos moleculares com maior reprodutibilidade e poder discriminatório, como a PFGE, podem ser mais úteis em investigações epidemiológicas, pois a PFGE oferece um perfil de restrição reproduzível de grandes fragmentos de DNA bacteriano 7 .…”