Strain Typing of Trichosporon asahii Clinical Isolates by Random Amplification of Polymorphic DNA (RAPD) Analysis

Premamalini, Thayanidhi; Rajyoganandh, Vijayaraman; Vijayakumar, R.; Hemanth, Veena; Kindo, Anupma Jyoti; Marak, Rungmei S. K.

doi:10.1055/s-0041-1731111

Cited by 1 publication

(1 citation statement)

References 39 publications

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“…The primer used in RAPD-PCR was primer 272 (59-AGCGGGCCAA-39). 11 The amplification volume was 20 mL, which included 2 mL primers (100 mmol/L), 10 mL 23 PowerTaq PCR Master Mix, 1.5 mL template, and 6.5 mL double-distilled H 2 O. The parameters of RAPD-PCR were as follows: 4 cycles of 5 minutes at 94 C, 5 minutes at 36 C, and 5 minutes at 72 C; 30 cycles of 1 minute at 94 C, 1 minute at 36 C, and 2 minutes at 72 C; and a final extension at 72 C for 10 minutes.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Pseudomonas aeruginosa Phages with a Broad Host Spectrum from Hospital Sewage Systems and Their Therapeutic Effect in a Mouse Model

Dai

Yang

Lou

et al. 2023

The American Journal of Tropical Medicine and Hygiene

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This study aimed to isolate and characterize phages as an alternative treatment of multidrug- or pan-drug-resistant Pseudomonas aeruginosa. Phage titers and bacterial densities correlated, with the phages disappearing after bacteria were eliminated. We isolated phages in filtered sewage water by a double-layered agar spot test. Fifty-eight P. aeruginosa strains were used to screen the host spectrum of the 14 phages isolated. Random amplification of polymorphic DNA-typing polymerase chain reaction was used to analyze the genomic homologies of the 58 host bacteria strains and four phages with a broad host spectrum. Transmission electron microscopy was used to observe the morphology of the four phages with a broad host spectrum. Mice with intraabdominal P. aeruginosa infection were used as an in vivo animal model to investigate the therapeutic effect of the selected phage. Four virulent phages with a broad host spectrum specific to P. aeruginosa strains were isolated. They were all double-stranded DNA viruses and belonged to four different genotypes. The test curve showed that phage I had the highest adsorption rate, the shortest latent period, and the largest burst size. The infected mouse model indicated that small doses of phage I could prevent the death of infected mice. Phage titers and bacterial densities correlated, with phages disappearing after bacteria were eliminated. Phage I was the most effective and promising treatment of drug-resistant P. aeruginosa.

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Section: Methodsmentioning

confidence: 99%