Strain-specific type 1 poliovirus-neutralizing monoclonal antibodies

Couillin, P.; Crainic, R; Cabau, N; Horodniceanu, F; Boué, A

doi:10.1016/s0769-2617(82)80010-5

Cited by 15 publications

(15 citation statements)

References 10 publications

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“…Strains GC069, GC082, GC128, GC135, and GC440 all demonstrated complete resistance to microcin 25 (22,35); the rfaQ mutation in CS2774 results in a partial deep rough phenotype (22,35 flzA(pBB440), a strain which expressed diminished amounts of the chimeric protein ( Fig. 3 and 4) terized determinant (19,42,43) against which MAbs are available (3,12). In addition, its utility as a reporter epitope for insertion into outer membrane proteins is well documented (8,10,11,41).…”

Section: Methodsmentioning

confidence: 99%

Genetic insertion and exposure of a reporter epitope in the ferrichrome-iron receptor of Escherichia coli K-12

et al. 1994

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The ferrichrome-iron receptor of Escherichia coli K-12 is FhuA (Me, 78,992), the first component of an energy-dependent, high-affinity iron uptake pathway. FhuA is also the cognate receptor for bacteriophages T5, Ti, +80, and UC-1, for colicin M and microcin 25, and for albomycin. To probe the topological organization of FhuA which enables recognition of these different ligands, we generated a library of 16 Under conditions of iron deprivation, many gram-negative bacteria derepress the expression of a class of surface receptors called iron-regulated outer membrane proteins. The function of these proteins is to bind specific siderophores and to initiate their internalization, thereby enabling the cell to satisfy its iron requirement (5). Siderophores have widely differing structures, but all share a high affinity for the ferric ion (24). Assimilation of the ferric siderophore requires a specific receptor as well as other periplasmic and cytoplasmic membrane proteins, including TonB (28). A cytoplasmic membrane protein complex including TonB, ExbB, and ExbD is thought to couple the electrochemical potential of the cytoplasmic membrane to the outer membrane siderophore receptor, a step which allows subsequent translocation of the bound ligand into the periplasm (37,38 proteins, including FepA (26), FhuA (23), FoxA (1), and LamB (9). These models incorporate some structural elements in common: amphiphilic sequences constitute antiparallel 3 sheets which traverse the outer membrane; intervening sequences are thought to constitute loops that are exposed at the cell surface or in the periplasm.Predictions of outer membrane protein topology can be evaluated with a number of tools, including monoclonal antibodies (MAbs) (26,34,39), insertion and deletion mutagenesis (4, 6, 7, 23), and reporter protein fusions (27)

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Section: Methodsmentioning

confidence: 99%

Genetic insertion and exposure of a reporter epitope in the ferrichrome-iron receptor of Escherichia coli K-12

et al. 1994

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“…Thus, this vascular muscle, in which Ca2channels are considered the most important 25 for membrane excitation (1)(2)(3)13), has two types of Ca2+ channels and a Na+ channel. These Ca2+ channels are similar in voltage ranges and kinetics to those characterized in neurons, cardiac muscle, and other cell types (4).…”

Section: Calcium and Sodium Channels In Spontaneously Contracting Vasmentioning

confidence: 99%

Calcium and Sodium Channels in Spontaneously Contracting Vascular Muscle Cells

Sturek

Hermsmeyer

1986

Science

170

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Electrophysiological recordings of inward currents from whole cells showed that vascular muscle cells have one type of sodium channel and two types of calcium channels. One of the calcium channels, the transient calcium channel, was activated by small depolarizations but then rapidly inactivated. It was equally permeable to calcium and barium and was blocked by cadmium, but not by tetrodotoxin. The other type, the sustained calcium channel, was activated by larger depolarizations, but inactivated very little; it was more permeable to barium than calcium. The sustained calcium channel was more sensitive to block by cadmium than the transient channel, but also was not blocked by tetrodotoxin. The sodium channel inactivated 15 times more rapidly than the transient calcium channel and at more negative voltages. This sodium channel, which is unusual because it is only blocked by a very high (60 microM) tetrodotoxin concentration but not by cadmium, is the first to be characterized in vascular muscle, and together with the two calcium channels, provides a basis for different patterns of excitation in vascular muscles.

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“…BALB/c mice (8 weeks old) were immunized with either 2 ,ug of HBsAg (mouse 1) or 30 Wg of HBsPolioAg (mice 2 to 4) by intraperitoneal injection using a 50% emulsion of Freund's complete adjuvant, repeated 2 weeks later with Freund's incomplete adjuvant. After 3 weeks each mouse received an injection of Sp2/0-Agl4 mouse myeloma cells (25) followed immediately by an intravenous booster injection without adjuvant. Mouse 5 was mock-immunized without antigen.…”

Section: Supported By Natural Sciences and Engineeringmentioning

confidence: 99%

A Poliovirus Neutralization Epitope Expressed on Hybrid Hepatitis B Surface Antigen Particles

Delpeyroux

Chenciner

Lim

et al. 1986

Science

Self Cite

111

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The hepatitis B virus (HBV) envelope protein carrying the surface antigen (HBsAg) is assembled with cellular lipids in mammalian cells into empty viral envelopes. In a study to evaluate the capacity of such particles to present foreign peptide sequences in a biologically active form, in-phase insertions were created in the S gene encoding the major envelope protein. One of the sequences inserted was a synthetic DNA fragment encoding a poliovirus neutralization epitope. Mammalian cells expressing the modified gene secreted hybrid particles closely resembling authentic 22-nanometer HBsAg particles. These particles reacted with a poliovirus-specific monoclonal antibody and induced neutralizing antibodies against poliovirus. The results indicate that empty viral envelopes of HBV may provide a means for the presentation of peptide sequences and for their export from mammalian cells.

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Strain-specific type 1 poliovirus-neutralizing monoclonal antibodies

Cited by 15 publications

References 10 publications

Genetic insertion and exposure of a reporter epitope in the ferrichrome-iron receptor of Escherichia coli K-12

Genetic insertion and exposure of a reporter epitope in the ferrichrome-iron receptor of Escherichia coli K-12

Calcium and Sodium Channels in Spontaneously Contracting Vascular Muscle Cells

A Poliovirus Neutralization Epitope Expressed on Hybrid Hepatitis B Surface Antigen Particles

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