Strain isolation and optimization of process parameters for bioconversion of glycerol to lactic acid

Hong, An-An; Cheng, Ke-Ke; Peng, Feng; Zhou, Sheng; Sun, Yuankui; Liu, Canming; Liu, Dehua

doi:10.1002/jctb.2209

Cited by 68 publications

(35 citation statements)

References 28 publications

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“…Fifty grams per liter of L-lactic acid (99.9% chiral purity) was produced from 56 g/L of waste glycerol with a yield of 0.91 mol/mol glycerol. Other than engineered strains, lactic acid production was also observed in glycerol fermentation by E. coli AC-521, a wild-type soil bacterium [27]. The yield reached 0.9 mole lactic acid per mole consumed glycerol with a final concentration of about 85.8 g/L (0.97 g/L/h productivity), however no data about chiral purity was presented in this study.…”

Section: Escherichia Speciesmentioning

confidence: 75%

“…This strain was shown to be capable of producing 1 mole of ethanol and 1 mole of hydrogen gas per mole of glycerol consumed [26] (Table 1). Lactic acid can be produced during glycerol fermentation by some E. coli strains as an alternative to NAD + regeneration in the absence of external electron acceptors [27]. Lactic acid has many applications as a food additive, acidulant, as well in the production of biodegradable polylactic acid [81,82].…”

Section: Escherichia Speciesmentioning

confidence: 99%

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Microbial Conversion of Waste Glycerol from Biodiesel Production into Value-Added Products

Lesnik

Liang

2013

Energies

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Biodiesel has gained a significant amount of attention over the past decade as an environmentally friendly fuel that is capable of being utilized by a conventional diesel engine. However, the biodiesel production process generates glycerol-containing waste streams which have become a disposal issue for biodiesel plants and generated a surplus of glycerol. A value-added opportunity is needed in order to compensate for disposal-associated costs. Microbial conversions from glycerol to valuable chemicals performed by various bacteria, yeast, fungi, and microalgae are discussed in this review paper, as well as the possibility of extending these conversions to microbial electrochemical technologies.

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Section: Escherichia Speciesmentioning

confidence: 75%

Section: Escherichia Speciesmentioning

confidence: 99%

Microbial Conversion of Waste Glycerol from Biodiesel Production into Value-Added Products

Lesnik

Liang

2013

Energies

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“…But only a few papers have been published on the use of glycerol as carbon source for lactic acid production [6,17]. For instance, Hong et al [17] compared eight bacterial strains for lactic acid production from glycerol.…”

Section: Fermentation Of Glycerolmentioning

confidence: 99%

Analysis of the Production Process of Optically Pure d-Lactic Acid from Raw Glycerol Using Engineered Escherichia coli Strains

Posada

Cardona

González

2011

Appl Biochem Biotechnol

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Glycerol has become an ideal feedstock for producing fuels and chemicals. Here, five technological schemes for optically pure D: -lactic acid production from raw glycerol were designed, simulated, and economically assessed based on five fermentative scenarios using engineered Escherichia coli strains. Fermentative scenarios considered different qualities of glycerol (pure, 98 wt.%, and crude, 85 wt.%) with concentrations ranging from 20 to 60 g/l in the fermentation media, and two fermentation stages were also analyzed. Raw glycerol (60 wt.%) was considered as the feedstock feeding the production process in all cases; then a purification process of raw glycerol up to the required quality was required. Simulation processes were carried out using Aspen Plus, while economic assessments were performed using Aspen Icarus Process Evaluator. D: -Lactic acid recovery and purification processes were based on reactive extraction with tri-n-octylamine using dichloromethane as active extractant agent. The use of raw glycerol represents only between 2.4% and 7.8% of the total production costs. Also, the total production costs obtained of D: -lactic acid in all cases were lower than its sale price indicating that these processes are potentially profitable. Thus, the best configuration process requires the use of crude glycerol diluted at 40 g/l with total glycerol consumption and with D: -lactic acid recovering by reactive extraction. The lowest obtained total production cost was 1.015 US$/kg with a sale price/production cost ratio of 1.53.

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“…In this topic, Hong et al (2009), determined optimal fermentation conditions for Escherichia coli AC-521 reaching lactic acid concentration up to 85.8 g/L when fed-batch culture was applied with pure glycerol as carbon source. Choubisa et al (2012), produced lactic acid from crude glycerol in batch cultures using strains of Lactobacillus delbrueckii reaching a concentration of 4.37 g/L and from pure glycerol using Lactococcus lactis, reaching concentrations of 2.26 g/L.…”

Section: Introductionmentioning

confidence: 99%

Unstructured kinetic model of batch fermentation of USP glycerol for lactic acid production

Gamboa-Rueda

Lizcano-González

Ordoñez-Supelano

et al. 2015

CT&F Cienc. Tecnol. Futuro

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This study show the capability of Lactobacillus rhamnosus ATCC 7469 to produce lactic acid using crude glycerol from biodiesel production as carbon source; in addition, a kinetic model that describes the behaviour of the fermentation process using USP glycerol as substrate was proposed and developed. The strain was adapted to the new carbon source by doing successive cultures, the substrate conversion was up to 94.5% after 24 hours of fermentation using crude glycerol as under initial conditions of substrate concentration (crude glycerol) and pH of 8 g/L and 6.5, respectively. Then, the influence of initial pH (pH o ) and concentration of substrate (So) were evaluated by fermentation tests of USP glycerol. The optimal value of volumetric lactic acid productivity (Qv) achieved from this study was 0.087 g . L -1. h -1 for initial pH o = 6.5 and initial substrate concentration S o = 6 g/L. Lastly, the kinetics parameters of an unstructured and not segregated model (Aiba type equation for growth rate expression), were adjusted with an average fit degree of 88% for all the initial conditions, using USP glycerol. In addition, the kinetic parameters are laid out as function of initial pH and substrate concentration. 82E ste trabalho demonstrou a capacidade do Lactobacillus rhamnosus ATCC 7469 para a produção de ácido láctico usando glicerol quando oriundo da indústria do biodiesel como fonte de carbono, adicionalmente, um modelo cinético foi proposto e desenvolvido com a finalidade de descrever o processo de fermentação usando glicerol USP como substrato. A cepa foi adaptada à nova fonte de carbono utilizando culturas sucessivas, a conversão do substrato foi de até 94.5% após 24 horas de fermentação em condições iniciais de substrato de 8 g/L e pH inicial de 6.5, respectivamente. Posteriormente, a influência do pH inicial (pH o ) e a concentração de substrato inicial (S o ) foram avaliadas mediante ensaios fermentativos de glicerol USP. O valor ótimo de produtividade volumétrica de ácido láctico (Qv) conseguido neste trabalho foi de 0.087 g . L -1. h -1 para um pH inicial de pH o = 6.5 e uma concentração inicial de substrato S o = 6 g/L. Finalmente, os parâmetros cinéticos de um modelo não estruturado e não segregado (equação tipo Aiba para a velocidade de crescimento), foram otimizados atingindo um grau de ajuste de 88% para todas as condições iniciais, usando glicerol USP. Adicionalmente, os parâmetros cinéticos são apresentados como função do pH inicial e a concentração inicial do substrato. E n este trabajo se demostró la capacidad de Lactobacillus rhamnosus ATCC 7469 para producir ácido láctico usando glicerol crudo proveniente de la industria del biodiesel como fuente de carbono, además se propuso y se desarrolló un modelo cinético que describe el proceso de fermentación usando glicerol USP como sustrato. La cepa fue adaptada a la nueva fuente de carbono utilizando cultivos sucesivos, la conversión de sustrato fue de hasta 94.5% después de 24 horas de fermentación en condiciones iniciales de sustrato de 8 g/L y ...

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Strain isolation and optimization of process parameters for bioconversion of glycerol to lactic acid

Cited by 68 publications

References 28 publications

Microbial Conversion of Waste Glycerol from Biodiesel Production into Value-Added Products

Microbial Conversion of Waste Glycerol from Biodiesel Production into Value-Added Products

Analysis of the Production Process of Optically Pure d-Lactic Acid from Raw Glycerol Using Engineered Escherichia coli Strains

Unstructured kinetic model of batch fermentation of USP glycerol for lactic acid production

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