Strain engineering for microbial production of value-added chemicals and fuels from glycerol

Westbrook, Adam; Miscevic, Dragan; Kilpatrick, Shane; Bruder, Mark; Moo‐Young, Murray; Chou, C. Perry

doi:10.1016/j.biotechadv.2018.10.006

Cited by 35 publications

(13 citation statements)

References 331 publications

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“…For example, cell growth can be maintained or enhanced upon the addition of glycerol to anaerobic glucose cultures of Lactobacillus sp. changing their metabolism (Westbrook et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Metabolic Characterization of Supernatants Produced by Lactobacillus spp. With in vitro Anti-Legionella Activity

et al. 2019

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Legionella pneumophila is an organism of public health interest for its presence in water supply systems and other humid thermal habitats. In this study, ten cell-free supernatants produced by Lactobacillus strains were evaluated for their ability to inhibit L. pneumophila strains isolated from hot tap water. Production of antimicrobial substances by Lactobacillus strains were assessed by agar well diffusion test on BCYE agar plates pre-inoculated with L. pneumophila. Cell-free supernatants (CFS) showed antimicrobial activity against all Legionella strains tested: L. rhamnosus and L. salivarius showed the highest activity. By means of a proton-based nuclear magnetic resonance ( 1 H-NMR) spectroscopy, we detected and quantified the Lactobacillus metabolites of these CFSs, so to gain information about which metabolic pathway was likely to be connected to the observed inhibition activity. A panel of metabolites with variations in concentration were revealed, but considerable differences among inter-species were not showed as reported in a similar work by Foschi et al. (2018) . More than fifty molecules belonging mainly to the groups of amino acids, organic acids, monosaccharides, ketones, and alcohols were identified in the metabolome. Significant differences were recorded comparing the metabolites found in the supernatants of strains grown in MRS with glycerol and the same strains grown in MRS without supplements. Indeed, pathway analysis revealed that glycine, serine and threonine, pyruvate, and sulfur metabolic pathways had a higher impact when strains were grown in MRS medium with a supplement such as glycerol. Among the metabolites identified, many were amino acids, suggesting the possible presence of bacteriocins which could be linked to the anti- Legionella activity shown by cell-free supernatants.

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“…For example, cell growth can be maintained or enhanced upon the addition of glycerol to anaerobic glucose cultures of Lactobacillus sp. changing their metabolism (Westbrook et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Metabolic Characterization of Supernatants Produced by Lactobacillus spp. With in vitro Anti-Legionella Activity

et al. 2019

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“…The predilection for research and development of bioplastic production by heterotrophic bacteria is justified by the considerable production and accumulation of PHA in their cells, with some species such as Cupriavidus metallidurans and E. coli , wild or genetically modified, being used for the production of PHA on a larger scale [ 47 , 48 ]. Despite the good production of biopolymer by these strains, an operational problem hinders its use at an industrial level: the need for more elaborated carbon sources, which can compete with the food sector [ 49 ], and are expensive with the cost of nutritional inputs reaching up to 50% of production costs [ 50 ].…”

Section: Bioplasticsmentioning

confidence: 99%

“…One way to mitigate these high costs in cultivation is using renewable and cheap carbon sources, such as domestic and industrial waste, which applies to heterotrophic bacteria [ 40 , 41 , 48 ] as well as photosynthetic organisms, that require fewer nutrients for their growth and production of biomass and biotechnological metabolites, such as bioplastic [ 51 , 52 ], thus, producing biopolymers basically from light and CO 2 [ 14 ]; in this context, we can understand the potential of the so-called blue algae, the cyanobacteria.…”

Section: Bioplasticsmentioning

confidence: 99%

“…The use of agricultural residues as a carbon source or for supplementation of other nutrients has already been studied in wild and engineered organisms, such as heterotrophic bacteria and eukaryotic microalgae, in addition to cyanobacteria [ 48 , 152 ], being well assimilated by them in the production of metabolites for various industries, reducing input costs as well as agro-industrial waste. It is a logical path to use wastewater for the cultivation of cyanobacteria given their nutritional preferences, as the blooms of this phylum mostly occur in environments with an abundance of phosphorus and nitrogen, which are also widely found in aquaculture waste [ 153 , 154 ].…”

Section: Cyanobacteriamentioning

confidence: 99%

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Cyanobacterial Polyhydroxyalkanoates: A Sustainable Alternative in Circular Economy

2020

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Conventional petrochemical plastics have become a serious environmental problem. Its unbridled use, especially in non-durable goods, has generated an accumulation of waste that is difficult to measure, threatening aquatic and terrestrial ecosystems. The replacement of these plastics with cleaner alternatives, such as polyhydroxyalkanoates (PHA), can only be achieved by cost reductions in the production of microbial bioplastics, in order to compete with the very low costs of fossil fuel plastics. The biggest costs are carbon sources and nutrients, which can be appeased with the use of photosynthetic organisms, such as cyanobacteria, that have a minimum requirement for nutrients, and also using agro-industrial waste, such as the livestock industry, which in turn benefits from the by-products of PHA biotechnological production, for example pigments and nutrients. Circular economy can help solve the current problems in the search for a sustainable production of bioplastic: reducing production costs, reusing waste, mitigating CO2, promoting bioremediation and making better use of cyanobacteria metabolites in different industries.

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“…Recently, global markets witnessed remarkable growth in the biodiesel industry that subsequently stimulated research toward utilization of byproduct glycerol (Vivek et al, 2017). Due to its abundance, high-reduction ability, non-toxicity, and low cost, glycerol is regarded as a favorable carbon substrate for numerous industrial microbes, including Escherichia coli (Westbrook et al, 2019), Saccharomyces cerevisiae (Xiberras et al, 2019), B. subtilis (Fan et al, 2018), and Pseudomonas species (Pobletecastro et al, 2020) to produce various types of metabolites. Glycerol is typically not treated as a carbon resource for C. glutamicum-based bioprocesses due to the absence of glycerol oxidation pathway enzymes in this strain.…”

Section: Glycerolmentioning

confidence: 99%

Recent Progress on Chemical Production From Non-food Renewable Feedstocks Using Corynebacterium glutamicum

Zhang

Jiang

et al. 2020

Front. Bioeng. Biotechnol.

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Due to the non-renewable nature of fossil fuels, microbial fermentation is considered a sustainable approach for chemical production using glucose, xylose, menthol, and other complex carbon sources represented by lignocellulosic biomass. Among these, xylose, methanol, arabinose, glycerol, and other alternative feedstocks have been identified as superior non-food sustainable carbon substrates that can be effectively developed for microbe-based bioproduction. Corynebacterium glutamicum is a model gram-positive bacterium that has been extensively engineered to produce amino acids and other chemicals. Recently, in order to reduce production costs and avoid competition for human food, C. glutamicum has also been engineered to broaden its substrate spectrum. Strengthening endogenous metabolic pathways or assembling heterologous ones enables C. glutamicum to rapidly catabolize a multitude of carbon sources. This review summarizes recent progress in metabolic engineering of C. glutamicum toward a broad substrate spectrum and diverse chemical production. In particularly, utilization of lignocellulosic biomass-derived complex hybrid carbon source represents the futural direction for non-food renewable feedstocks was discussed.

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Strain engineering for microbial production of value-added chemicals and fuels from glycerol

Cited by 35 publications

References 331 publications

Metabolic Characterization of Supernatants Produced by Lactobacillus spp. With in vitro Anti-Legionella Activity

Metabolic Characterization of Supernatants Produced by Lactobacillus spp. With in vitro Anti-Legionella Activity

Cyanobacterial Polyhydroxyalkanoates: A Sustainable Alternative in Circular Economy

Recent Progress on Chemical Production From Non-food Renewable Feedstocks Using Corynebacterium glutamicum

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