Strain distribution in an elastic substrate vibrated in a bioreactor for vocal fold tissue engineering

Titze, Ingo R.; Broadhead, Kelly; Tresco, Patrick A.; Gray, Steven D.

doi:10.1016/j.jbiomech.2004.10.011

Cited by 11 publications

(5 citation statements)

References 11 publications

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“…For example, the turbulent media flow and the inability to accommodate multiple samples limit the usage of Wolchok bioreactor in tissue engineering applications. Limitations of the Hitchcock bioreactor, on the other hand, include non-uniform strain distribution along the porous substrate,[212] the need for a large number of connectors and bars to transmit forces and the possibility of individual components to resonate at various frequencies. Finally, because the Tong bioreactor relies on the anchored silicone elastomer to transmit the oscillatory air pressure to the cellular constructs, the vibration is acoustic rather than aerodynamic, the vibration amplitude is small, and the collision forces are absent.…”

Section: Bioreactor Developmentsmentioning

confidence: 99%

Tissue engineering-based therapeutic strategies for vocal fold repair and regeneration

Stiadle

Lau

et al. 2016

Biomaterials

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Vocal folds are soft laryngeal connective tissues with distinct layered structures and complex multicomponent matrix compositions that endow phonatory and respiratory functions. This delicate tissue is easily damaged by various environmental factors and pathological conditions, altering vocal biomechanics and causing debilitating vocal disorders that detrimentally affect the daily lives of suffering individuals. Modern techniques and advanced knowledge of regenerative medicine have led to a deeper understanding of the microstructure, microphysiology, and micropathophysiology of vocal fold tissues. State-of-the-art materials ranging from extracecullar-matrix (ECM)-derived biomaterials to synthetic polymer scaffolds have been proposed for the prevention and treatment of voice disorders including vocal fold scarring and fibrosis. This review intends to provide a thorough overview of current achievements in the field of vocal fold tissue engineering, including the fabrication of injectable biomaterials to mimic in vitro cell microenvironments, novel designs of bioreactors that capture in vivo tissue biomechanics, and establishment of various animal models to characterize the in vivo biocompatibility of these materials. The combination of polymeric scaffolds, cell transplantation, biomechanical stimulation, and delivery of antifibrotic growth factors will lead to successful restoration of functional vocal folds and improved vocal recovery in animal models, facilitating the application of these materials and related methodologies in clinical practice.

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Section: Bioreactor Developmentsmentioning

confidence: 99%

Tissue engineering-based therapeutic strategies for vocal fold repair and regeneration

Stiadle

Lau

et al. 2016

Biomaterials

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“…Our data demonstrate maximum accelerations of 9.2-334 m/s 2 at 100-250 Hz by using different recoil materials with the TRB-multiwell system. These accelerations are much lower than predicted vocal fold accelerations using three-mass computer simulation or stroboscopy data (1000-4000 m/s 2 at 100-400 Hz) [20,21] but they are the highest values generated by any bioreactor to date [7,8,10,[22][23][24][25][26]. Stiff recoil materials provide accelerations in the order of 300 m/s 2 above 100 Hz and more compliant materials provide 100 m/s 2 at 28-100 Hz vibrations.…”

Section: Discussionmentioning

confidence: 91%

A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies

et al. 2014

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To mimic in vivo vibration of vocal fold cells, we studied the controllability and range of frequency, acceleration, duration, and shear stress in a new bioreactor attachment. The custom multiwell disc appliance fits into a commercially built rheometer, together termed a torsional rheometer bioreactor (TRB). Previous attachments to the TRB were capable of 50-100 Hz vibrations at relatively high strains but were limited to single-sample experiments. The TRB-multiwell disc system accommodates 20 samples in partially fluid-filled wells in an aseptic environment delivering three different acceleration conditions to different samples simultaneously. Frequency and amplitude used to calculate acceleration along with duration and shear stress were controllable and quantifiable using a combination of built-in rheometer sensors, manufacturer software, and smooth particle hydrodynamics (SPH) simulations. Computed shear stresses at the well bottom using SPH in two and three dimensions were verified with analytical approximations. Results demonstrate capabilities of the TRB-multiwell disc system that, when combined with computational modeling, provide quantifiable vibration parameters covering frequencies 0.01-250 Hz, accelerations of 0.02-300 m/s 2 , and shear stresses of 0.01-1.4 Pa. It is

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“…It was used in the present study, because TFX had similar properties to vocal fold tissues and was well characterized as biocompatible. TFX has been used for synthetic vascular grafts, in vitro tissue engineering studies ͑Klemuk et al, 2001;Mulder et al, 1998;Titze et al, 2005;Titze et al, 2004a;Vara et al, 2005;Webb et al, 2003͒, and …”

Section: A Uniform Cell Distributionmentioning

confidence: 99%

“…Force application to tissues in a bioreactor is quite common, but quantifying vibrational stresses has been attempted in very few bioreactors ͑Bacabac et Tanaka et al, 2003;Titze et al, 2004b;Titze et al, 2005;Titze et al, 2004a͒. Bacabac and The rheometer bioreactor used in the present investigation is capable of delivering vibrational shear strains observed during vocal fold movement and directly measuring viscoelastic properties.…”

Section: Vibrational Stresses Quantifiedmentioning

confidence: 99%

Cell viability viscoelastic measurement in a rheometer used to stress and engineer tissues at low sonic frequencies

Klemuk

Jaiswal

Titze

2008

The Journal of the Acoustical Society of America

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Effects of vibration on human vocal fold extracellular matrix composition and the resultant tissue viscoelastic properties are difficult to study in vivo. Therefore, an in vitro bioreactor, simulating the in vivo physiological environment, was explored. A stress-controlled commercial rheometer was used to administer shear vibrations to living tissues at stresses and frequencies corresponding to male phonation, while simultaneously measuring tissue viscoelastic properties. Tissue environment was evaluated and adjustments made in order to sustain cell life for short term experimentation up to 6 h. Cell nutrient medium evaporation, osmolality, pH, and cell viability of cells cultured in three-dimensional synthetic scaffolds were quantified under comparably challenging environments to the rheometer bioreactor for 4 or 6 h. The functionality of the rheometer bioreactor was demonstrated by applying three vibration regimes to cell-seeded three-dimensional substrates for 2 h. Resulting strain was quantified throughout the test period. Rheologic data and cell viability are reported for each condition, and future improvements are discussed.

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Strain distribution in an elastic substrate vibrated in a bioreactor for vocal fold tissue engineering

Cited by 11 publications

References 11 publications

Tissue engineering-based therapeutic strategies for vocal fold repair and regeneration

Tissue engineering-based therapeutic strategies for vocal fold repair and regeneration

A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies

Cell viability viscoelastic measurement in a rheometer used to stress and engineer tissues at low sonic frequencies

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