Strain differences in the cytotoxic activity and TNF production of murine macrophages stimulated by lentinan

Kerékgyártó, C; Virág, Lászó; Tankó, László B.; Chihara, Goro; Fachet, J

doi:10.1016/s0192-0561(96)00038-0

Cited by 39 publications

(25 citation statements)

References 15 publications

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“…The glucans screened by the current system included branched (133)-␤-D-glucans such as laminarin, paramylon, schizophyllan, lentinan, bakers' yeast ␤-glucan, barley ␤-glucan, and krestin. Although schizophyllan, lentinan, and krestin are known as BRM and have been reported to induce the production of nitric oxide and/or TNF-␣ in macrophages (28,42,43), by our present assay system we could not detect significant NF-B-stimulating activities of these branched (133)-␤-D-glucans at concentrations of 100 g/ml with or without NaOH pretreatment. Instead, another linear (133)-␤-D-glucan, 2 K. Kataoka and T. Muta, unpublished data.…”

Section: Discussioncontrasting

confidence: 60%

Activation of Macrophages by Linear (1→3)-β-d-Glucans

Kataoka¹,

Muta²,

Yamazaki³

et al. 2002

Journal of Biological Chemistry

195

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Section: Discussioncontrasting

confidence: 60%

Activation of Macrophages by Linear (1→3)-β-d-Glucans

Kataoka¹,

Muta²,

Yamazaki³

et al. 2002

Journal of Biological Chemistry

195

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“…A co-culture experiment was performed as described by Tanoue et al 16) TNFcontents were quantified by cytotoxicity assay with L929 cells (actinomycin D-treated murine fibroblast cell line) using murine rTNF-as the standard, as described by Kerékgyártó et al 10) Semi-quantitative RT-PCR was performed as described previously. 16) To semi-quantify the induction of IL-8 mRNA expression in Caco-2 cells, IL-8 mRNA expression levels were normalized relative to the expression of GAPDH mRNA, and IL-8/GAPDH ratios were calculated by densitometric analysis using Image J software.…”

Section: )mentioning

confidence: 99%

“…8) Lentinan stimulates natural killer (NK) cell activity 9) and several macrophage functions, e.g., the release of tumor necrosis factor (TNF)- 10) and interleukin-1.…”

mentioning

confidence: 99%

Different Suppressive Effects of Fucoidan and Lentinan on IL-8 mRNA Expression inin VitroGut Inflammation

Mizuno¹,

Nishitani²,

Hashimoto³

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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A system for assessing the anti-inflammatory effects of food factors was developed by establishing a coculture system with intestinal epithelial Caco-2 cells and macrophage RAW264.7 cells. The results indicate that fucoidan and lentinan exhibited different suppressive effects on interleukin-8 (IL-8) mRNA expression in Caco-2 through tumor necrosis factor-(TNF-) production from RAW264.7 stimulated with lipopolysaccharide (LPS).

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“…It has been shown to stimulate natural killer cell activity (20 -22), macrophage/monocyte functions (secreting IL-1 and superoxide anion), phagocytosis, and cytotoxicity (23)(24)(25)(26)(27)(28). Lentinan was found to elevate the cytotoxic activity and TNF secretion of macrophages in vitro and in vivo (16,29). Pretreatment of bone marrow macrophages with Lentinan results in increased production of NO in vitro (16).…”

mentioning

confidence: 99%

Immunomodulatory β-Glucan from Lentinus edodes Activates Mitogen-activated Protein Kinases and Nuclear Factor-κB in Murine RAW 264.7 Macrophages

Pan

Zhang

et al. 2011

Journal of Biological Chemistry

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Lentinan, a cell wall ␤-glucan from the fruiting bodies of Lentinus edodes, is well known to be a biological defense modifier, but the signal transduction pathway(s) induced by Lentinan have not been elucidated. In this study, we extracted Lentinan (LNT-S) by ultrasonication from Lentinus edodes and report that, in murine RAW 264.7 macrophages, LNT-S glucan activated NF-B p65 and triggered its nuclear translocation as determined by Western blotting. Moreover, LNT-S enhanced NF-B-luciferase activity in the Dual-Luciferase gene system assay. Its upstream signaling molecules, MAPKs such as ERK1/2 and JNK1/2, were shown to be activated by assessing the level of phosphorylation in a time-and concentration-dependent manner, but its downstream proinflammatory enzyme, inducible NOS, was not observed. The data evaluated using a TNF-␣ ELISA kit and Griess reagent further demonstrated that no proinflammatory mediators such as TNF-␣ and NO were produced by LNT-S stimulation in RAW 264.7 cells. In contrast, LPS significantly induced inducible NOS expression and increased NO and TNF-␣ production, which are associated with activation of the NF-B p65/p50 heterodimer complex. It is possible that LNT-S did not activate NF-B p65/p50, and the activation of NF-B p65 was not sufficient to stimulate cytokine production. These data demonstrate that LNT-S glucan carries out its immunomodulating activity by activating MAPK signaling pathways without secretion of TNF-␣ and NO.

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Strain differences in the cytotoxic activity and TNF production of murine macrophages stimulated by lentinan

Cited by 39 publications

References 15 publications

Activation of Macrophages by Linear (1→3)-β-d-Glucans

Activation of Macrophages by Linear (1→3)-β-d-Glucans

Different Suppressive Effects of Fucoidan and Lentinan on IL-8 mRNA Expression inin VitroGut Inflammation

Immunomodulatory β-Glucan from Lentinus edodes Activates Mitogen-activated Protein Kinases and Nuclear Factor-κB in Murine RAW 264.7 Macrophages

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