Strain-dependent resistance to allergen-induced lung pathophysiology in mice correlates with rate of apoptosis of lung-derived eosinophils

Abstract

Although exposed to similar allergic and environmental stimuli, not all humans develop asthma. Similarly, mouse strains vary in the degree of pathophysiology seen following induction of experimental asthma. Three mouse strains (CBA/Ca, BALB/c, and C57BL/6) were used to determine if the extent and duration of inflammation influenced the degree of lung tissue damage in an OVA-induced allergic asthma model. Airways obstruction, leukocyte infiltration, edema, eosinophil accumulation, and degranulation were less severe in wild-type (wt) CBA/Ca mice than wt BALB/c and C57BL/6 mice. F1 hybrids of CBA/Ca mice crossed with BALB/c or C57BL/6 mice had bronchoalveolar lavage leukocyte (BAL) and cell-free protein profiles similar to those of the respective disease-susceptible parental strain. IL-5 transgene expression on each of the three genetic backgrounds accentuated the difference between CBA/Ca and the other two strains. Importantly, even when overexpressing IL-5, CBA/Ca mice did not develop substantial airways obstruction. Eosinophils recovered from the airways of allergic wt and IL-5 transgenic (Tg) CBA/Ca mice entered apoptosis at a faster rate than eosinophils from the other parental strains and F1 hybrids. In contrast, eosinophils harvested from the peritoneal cavities of untreated CBA/Ca IL-5 Tg mice had a relatively low rate of apoptosis in vitro. The CBA/Ca mouse strain is therefore relatively resistant to experimental asthma, and this may be a consequence of a propensity for apoptosis of eosinophils recruited into the allergic lung. Restricting survival of a key effector cell may thus limit pathogenesis in this experimental model and in humans.

The Contraceptive Depot Medroxyprogesterone Acetate Impairs Mycobacterial Control and Inhibits Cytokine Secretion in Mice Infected with Mycobacterium tuberculosis

“…The increase in bacterial load could also be explained by the reduction in serum TNF-␣ concentration and the inability of the MLNs to produce this particular cytokine, as this cytokine is known to play an important role in the confinement of mycobacteria (46). Interstrain variations have been described for mice with regard to inflammatory and immunological functions (19,47). Macrophages from different mouse strains also exhibit different sensitivities to the glucocorticoid dexamethasone (19).…”

Eosinophils and Trichinella infection: toxic for the parasite and the host?

“…Recently, strain-dependent resistance in the asthma model in mice has been reported to correlate with the apoptosis rate of lung-derived eosinophils [50]. Another possibility might be the difference in the degree of eosinophil activation.…”

Role of aluminum adjuvant in producing an allergic rhinitis animal model

“…Recent studies found that making the aluminum adjuvant into nanometer form could induce the specific antibody in mice quickly and at a high level, and that compared with other forms of aluminum adjuvant, it could induce humoral immunity earlier (Frey et al, 1999). After consulting the relevant literature, we found the amount of OVA used in animal models generally ranges from 10 to 100 μg (Yamaki et al, 2005;Tumes et al, 2007;Liu et al, 2010;Oh et al, 2011), whereas the amount of Al(OH) 3 ranges from 1 and 5 mg. Moreover, there are several forms of Al(OH) 3 used in these experiments including powder, gel, solution, and other dosage forms (Yamaki et al, 2005;Zhao et al, 2005;Su et al, 2006;Tumes et al, 2007;Mo et al, 2011).…”

“…After consulting the relevant literature, we found the amount of OVA used in animal models generally ranges from 10 to 100 μg (Yamaki et al, 2005;Tumes et al, 2007;Liu et al, 2010;Oh et al, 2011), whereas the amount of Al(OH) 3 ranges from 1 and 5 mg. Moreover, there are several forms of Al(OH) 3 used in these experiments including powder, gel, solution, and other dosage forms (Yamaki et al, 2005;Zhao et al, 2005;Su et al, 2006;Tumes et al, 2007;Mo et al, 2011). Our study focused on these three dosage forms (powder, gel, and solution) in the production of an AR animal model.…”