Strain-Dependent Cellular Immune Responses in Cattle following Escherichia coli O157:H7 Colonization

Corbishley, Alexander; Ahmad, Nur Indah; Hughes, Kirsty; Hutchings, Michael R.; McAteer, Sean P.; Connelley, Timothy; Brown, Helen; Gally, David L.; McNeilly, Tom N.

doi:10.1128/iai.02462-14

Cited by 30 publications

(41 citation statements)

References 86 publications

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“…The up-regulation of CD36 (log2 fold change: 4.1) was reported to increase T-cell proliferation i n vitro 43 ; GPAM , also known as GPAT-1 , (log2 fold change: 2.4) was reported to increase T-cell proliferation in mice 44 . These findings are in agreement with previous findings that increased expression of genes involved in the proliferation of T-cells in the rectum of E. coli O157 challenged cattle 45 . However, different from our previous transcriptomic analysis of rectal tissues which suggested a potential decrease in both humoral and cell-mediated immune functions in SS 11 , our current study indicates potentially enhanced T-cell migration and proliferation at the distal jejunum and descending colon of SS.…”

Section: Discussionsupporting

confidence: 93%

Host mechanisms involved in cattle Escherichia coli O157 shedding: a fundamental understanding for reducing foodborne pathogen in food animal production

Wang

McAllister

Plastow

et al. 2017

Sci Rep

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The host mechanisms involved in Escherichia coli O157 super-shedding in cattle is largely unknown. In this study, the comparison of transcriptomes of intestinal tissues between super-shedders (SS) and cattle negative for E. coli O157 (NS) was performed, aiming to identify genes that are potentially associated with super-shedding. In total, 16,846 ± 639 (cecum) to 18,137 ± 696 (distal jejunum) were expressed throughout the intestine, with the expressed genes associated with immune functions more pronounced in the small intestine. In total, 351 differentially expressed (DE) genes were identified throughout the intestine between SS and NS, with 101 being up-regulated and 250 down-regulated in SS. Functional analysis revealed DE genes were involved in increased T-cell responses and cholesterol absorption in the distal jejunum and descending colon, and decreased B-cell maturation in the distal jejunum of SS. RNA-Seq based SNP discovery revealed that the mutations in seven DE genes involved in leukocyte activation and cholesterol transportation were associated with E. coli O157 shedding. Our findings suggest that T-cell responses and cholesterol metabolism in the intestinal tract may be associated with super-shedding phenomenon, and the SNPs in the DE genes are possibly associated with the observed gene expression difference between SS and NS.

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Section: Discussionsupporting

confidence: 93%

Host mechanisms involved in cattle Escherichia coli O157 shedding: a fundamental understanding for reducing foodborne pathogen in food animal production

Wang

McAllister

Plastow

et al. 2017

Sci Rep

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“…On the contrary, it has been observed that EHEC as wells as EPEC strains could suppress NF-κB and MAPK activation as well as IκB degradation, [13] and could inhibit the production of proinflammatory cytokines IL-8 and IL-6, early in the infection by different LEE- and non-LEE encoded effectors (Tir, NleB, NleC, NleD, NleE, NleH1 and NleH2) [14]. Clearance of EHEC O157 is associated with an up-regulation of Th-1 associated transcripts within the rectal mucosa, the principle site of colonization [10, 15], suggesting that a cellular component of the adaptive immune response may be important in EHEC O157 control.…”

Section: Introductionmentioning

confidence: 99%

Potential immunosuppressive effects of Escherichia coli O157:H7 experimental infection on the bovine host

Kieckens

Rybarczyk

Li³

et al. 2016

BMC Genomics

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Background: Enterohaemorrhagic Escherichia coli (EHEC), like E. coli O157:H7 are frequently detected in bovine faecal samples at slaughter. Cattle do not show clinical symptoms upon infection, but for humans the consequences after consuming contaminated beef can be severe. The immune response against EHEC in cattle cannot always clear the infection as persistent colonization and shedding in infected animals over a period of months often occurs. In previous infection trials, we observed a primary immune response after infection which was unable to protect cattle from reinfection. These results may reflect a suppression of certain immune pathways, making cattle more prone to persistent colonization after re-infection. To test this, RNA-Seq was used for transcriptome analysis of recto-anal junction tissue and ileal Peyer's patches in nine Holstein-Friesian calves in response to a primary and secondary Escherichia coli O157: H7 infection with the Shiga toxin (Stx) negative NCTC12900 strain. Non-infected calves served as controls. Results: In tissue of the recto-anal junction, only 15 genes were found to be significantly affected by a first infection compared to 1159 genes in the ileal Peyer's patches. Whereas, re-infection significantly changed the expression of 10 and 17 genes in the recto-anal junction tissue and the Peyer's patches, respectively. A significant downregulation of 69 immunostimulatory genes and a significant upregulation of seven immune suppressing genes was observed.

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“…To date there has been little empirical research on the interaction between F. hepatica and VTEC O157 in cattle. Recent evidence suggests a role for type 1 immune responses in control of VTEC O157 in cattle, with clearance of the bacteria from the bovine intestine associated with an up-regulation of T-helper type 1 associated transcripts within the rectal mucosa, the principle site of colonisation by this bacteria ( Corbishley et al, 2014; Naylor et al, 2003 ). On the other hand, F. hepatica is known to suppress type 1 immune responses and induce an anti-inflammatory or regulatory immune environment in the host ( Brady et al, 1999; Flynn et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

The feasibility of testing whether Fasciola hepatica is associated with increased risk of verocytotoxin producing Escherichia coli O157 from an existing study protocol

Hickey

Diggle

McNeilly

et al. 2015

Preventive Veterinary Medicine

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Strain-Dependent Cellular Immune Responses in Cattle following Escherichia coli O157:H7 Colonization

Cited by 30 publications

References 86 publications

Host mechanisms involved in cattle Escherichia coli O157 shedding: a fundamental understanding for reducing foodborne pathogen in food animal production

Host mechanisms involved in cattle Escherichia coli O157 shedding: a fundamental understanding for reducing foodborne pathogen in food animal production

Potential immunosuppressive effects of Escherichia coli O157:H7 experimental infection on the bovine host

The feasibility of testing whether Fasciola hepatica is associated with increased risk of verocytotoxin producing Escherichia coli O157 from an existing study protocol

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