Strain and cultivar specificity in the Agrobacterium-soybean interaction

Byrne, Michael C.; McDonnell, Raymond E.; Wright, Martha; Carnes, Michael G.

doi:10.1007/bf00040728

Cited by 77 publications

(32 citation statements)

References 59 publications

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“…TL-DNA alone or TL-DNA combined with TR-DNA might render cells of (Ooms et al, 1982). Also, auxins secreted by Ri transformed roots stimulate the development of roots from non-transformed cells present at the wound site or in explanted hairy root tumors (Byrne et al, 1986). PCR analysis of the four of 12 tumor-derived shoot strains showed no amplification of the expected 2.5 kbp bands, indicating that non-transformed cells were also present in the crown galls induced by A. rhizogenes and that the shoot strains were derived from those cells proliferating during cultures.…”

Section: Discussionmentioning

confidence: 99%

Genetic Transformation of Japanese Persimmon (Diospyros kaki L.) by Agrobacterium rhizogenes Wild Type Strain A4

Tao

Handa

Tamura

et al. 1994

Engei Gakkai zasshi

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Section: Discussionmentioning

confidence: 99%

Genetic Transformation of Japanese Persimmon (Diospyros kaki L.) by Agrobacterium rhizogenes Wild Type Strain A4

Tao

Handa

Tamura

et al. 1994

Engei Gakkai zasshi

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“…Another WHR strain, A28 1, was also examined because it was chromosomally isogenic to A348 but contained a succinamopine Ti plasmid. It was more virulent on some plant cultivars than the octopine plasmid strain, A348 (2). Insertion of Tn5 in the ipt locus (A338) had no effect on the necrotic response (Fig.…”

Section: Effect Of Morphological Correlates On the Necrotic Reactionmentioning

confidence: 96%

Physical, Chemical, Developmental, and Genetic Factors that Modulate the Agrobacterium-Vitis Interaction

Lowe¹,

Krul²

1991

Plant Physiol.

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Tumor formation in Vitis species and hybrids, incited by Agrobacterium tumefaciens, was altered by chemical, physical, developmental, and genetic variables. Knowledge of the effect of these variables was used to develop a stringent in vitro assay system to select parents for a study of genetic factors that modulate tumor formation. Tumor formation was reduced by short day preconditioning of assay plants and by inoculation of the morphological apex of isolated stem segments. Pretreatment of plants with auxin or cytokinin altered specificity in various combinations of strains and host genotypes. All Vitis species and hybrids formed tumors in response to strains designated as limited host range, but some displayed a necrotic reaction (cell death at and below site of inoculation) or a null response (same as the response to inoculation with an avirulent strain) to strains designated as wide host range (VC Knauf, CG Panagopoulos, EW Nester [1982] Phytopathology 72: 1545-1549). Screens of F1 progeny, derived from crosses of null, necrotic, and tumor-producing phenotypes, demonstrated that the null and the necrotic phenotypes were modulated by dominant and recessive host genes. The extent of cellular necrosis in the necrotic phenotype was modified by the morphological location of the inoculation site, by the presence of buds on the host stem, and by deletion of the tryptophane monooxygenase locus gene of the Ti-plasmid.The tumor-inducing plasmid ofAgrobacterium tumefaciens contains two regions, termed the virulence (vir) and tumorinducing DNA (T-DNA), that coordinately participate in the excision of DNA from the pathogen and its transfer into the host genome (11,17). The current model for infection of host cells by Agrobacterium predicts that phenolic metabolites produced by wounded plant cells activate plasmid vir genes of the bacterium (22). Once activated, the genes modulate the excision and packaging of T-DNA and facilitate its transfer into the host plant genome (17,22). Expression of T-DNA in host cells alters the endogenous levels of auxin, cytokinin, and ethylene (6,11,17), which results in the loss of cellular totipotency (11,17 3,17,27). Bacterial host range and virulence determinants have been attributed to structural and organizational differences in T-DNA loci (1,17,21,(27)(28)(29)30) and to structural differences in at least two vir loci (30,31). Some investigators suggest that chromosomal loci might also modulate host selectivity (17,25). The role of the host genome in Agrobacterium-host compatibility has not received as much consideration as that of the pathogen. However, intergeneric (3,12,25,26,29,30) and intraspecific and cultivar variations (1, 2, 7-9, 19, 23, 24, 27) in hostresponse to WHR and/or LHR strains have been reported. A preliminary analysis of Vitis hybrids showed that resistance to A. tumefaciens was a heritable trait (23).The primary goal of our research was to develop a system to study the interactions of bacterial virulence genes and host genes so that the molecular and bioch...

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“…The Agrobacterium-mediated gene transfer system would appear to have some advantages because soybean is a dicotyledonous plant species and thus is susceptible to Agrobacterium infection. However, infectivity tests show that not all Agrobacterium strains can readily infect soybean tissues (5,9,22). The Agrobacterium strains which have been 1212 found to be the most effective for the infection of soybean cultivars are those known as the nopaline-type, which encode for the synthesis of the novel amino acid nopaline (8).…”

mentioning

confidence: 99%

Transformation of Soybean (Glycine max) by Infecting Germinating Seeds with Agrobacterium tumefaciens

Chee¹,

Fober²,

Slightom³

1989

Plant Physiol.

133

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The transfer of genetic material into soybean tissue was accomplished by using an avirulent strain of Agrobacterlum tumefaciens which contained the binary vector pGA482. The method used for transformation requires no tissue culture steps as it involves the inoculation of the plumule, cotyledonary node, and adjacent cotyledon tissues of germinating seeds. The identification of neomycin phosphotransferase (NPT) II enzyme activity in the tissues of 16 (Ro) soybean plants indicated that the plant expressible Nos-NPT II gene, contained within the T-DNA region from pGA482, had been transferred at least into somatic tissues.Putative transformed Ro soybean plants were advanced to produce R1 plants which were also assayed for the presence of the transferred Nos-NPT II gene. The combined results of these assays indicated that about 0.7% of the surviving inoculated seeds yielded transformed tissues in the Ro plant, and that about 1/10 of these plants yielded transformed R1 plants. The presence of the Nos-NPT II gene in DNAs isolated from both Ro and R1 plant was demonstrated by using genomic blot hybridization and polymerase chain reaction methods. Integration of this gene into the soybean genome was demonstrated for three R1 soybean plants.

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Strain and cultivar specificity in the Agrobacterium-soybean interaction

Cited by 77 publications

References 59 publications

Genetic Transformation of Japanese Persimmon (Diospyros kaki L.) by Agrobacterium rhizogenes Wild Type Strain A4

Genetic Transformation of Japanese Persimmon (Diospyros kaki L.) by Agrobacterium rhizogenes Wild Type Strain A4

Physical, Chemical, Developmental, and Genetic Factors that Modulate the Agrobacterium-Vitis Interaction

Transformation of Soybean (Glycine max) by Infecting Germinating Seeds with Agrobacterium tumefaciens

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