Strahlenchemische Ver�nderung der Bakterien-Desoxyribonucleins�ure in vivo

Wacker, A.; Dellweg, H.; Weinblum, D.

doi:10.1007/bf00638304

Cited by 182 publications

(31 citation statements)

References 6 publications

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“…Herein, we report the crystal structure of a cis-syn thymine dimer in free (unbound) duplex DNA, providing a clear picture of the effects of dimer formation on DNA structure and base pairing since it was first identified in DNA in 1960 (3,4). Most notably, the dimer is found to bend DNA by 30°, in remarkable agreement with some of the early theoretical (19) and experimental (28) studies, and in addition has an unexpectedly wide minor groove.…”

supporting

confidence: 73%

“…Evidence for the formation of the thymine dimer in DNA was first obtained Ͼ40 years ago (3,4) and a few years later for cytosine-containing CPDs (5). Because of the mutagenic and protein-DNA-disrupting properties of CPDs, many organisms have evolved enzymes to specifically recognize and repair cis-syn dimers, such as Escherichia coli photolyase (6, 7), T4 endoV (8), as well as general repair enzymes such as E. coli uvrABC (9) and human excinuclease (10).…”

mentioning

confidence: 99%

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Crystal structure of a DNA decamer containing a cis-syn thymine dimer

Park

Zhang²,

Ren³

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

212

209

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It is well known that exposure to UV induces DNA damage, which is the first step in mutagenesis and a major cause of skin cancer. Among a variety of photoproducts, cyclobutane-type pyrimidine photodimers (CPD) are the most abundant primary lesion. Despite its biological importance, the precise relationship between the structure and properties of DNA containing CPD has remained to be elucidated. Here, we report the free (unbound) crystal structure of duplex DNA containing a CPD lesion at a resolution of 2.0 Å. Our crystal structure shows that the overall helical axis bends Ϸ30°t oward the major groove and unwinds Ϸ9°, in remarkable agreement with some previous theoretical and experimental studies. There are also significant differences in local structure compared with standard B-DNA, including pinching of the minor groove at the 3 side of the CPD lesion, a severe change of the base pair parameter in the 5 side, and serious widening of both minor and major groves both 3 and 5 of the CPD. Overall, the structure of the damaged DNA differs from undamaged DNA to an extent that DNA repair proteins may recognize this conformation, and the various components of the replicational and transcriptional machinery may be interfered with due to the perturbed local and global structure. The cis-syn pyrimidine dimer (cyclobutane-type pyrimidine photodimer, CPD) is the major photoproduct induced by UV light present in sunlight (1) and is one of the principal causes of skin cancer (2). Evidence for the formation of the thymine dimer in DNA was first obtained Ͼ40 years ago (3, 4) and a few years later for cytosine-containing CPDs (5). Because of the mutagenic and protein-DNA-disrupting properties of CPDs, many organisms have evolved enzymes to specifically recognize and repair cis-syn dimers, such as Escherichia coli photolyase (6, 7), T4 endoV (8), as well as general repair enzymes such as E. coli uvrABC (9) and human excinuclease (10). Additionally, dimers are efficiently repaired in transcription-coupled repair by virtue of their ability to block synthesis by RNA polymerases (11,12). CPDs also block DNA replication (13) and are efficiently bypassed in a nonmutagenic manner by DNA damage bypass polymerases such as E. coli pol V (14) and the recently discovered yeast (15) and human polymerase (16,17). There is also evidence that the mismatch repair system can recognize mismatches opposite thymine dimers (18). Understanding the mechanism by which these proteins recognize and process CPDs will be greatly aided by a structure for CPD-containing DNA.The efficiency of damage repair is likely to depend on the extent to which those changes alter the structure of the DNA, hence making it recognizable for the repair enzymes involved. It has been suggested that the binding affinities of the repair enzymes for the CPD-containing DNA depend on the degree of DNA unwinding or kinking caused by those lesions (19,20). The first evidence that CPD formation causes large alterations in the structure of DNA is offered by circular dichroism studies and...

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supporting

confidence: 73%

mentioning

confidence: 99%

Crystal structure of a DNA decamer containing a cis-syn thymine dimer

Park

Zhang²,

Ren³

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

212

209

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“…It has been demonstrated that thymidine dimers occur in DNA irradiated by short wave ultraviolet light (26). Ultraviolet-induced alterations of cellular DNA can also be demonstrated in vivo (24).…”

Section: Resultsmentioning

confidence: 99%

Ultraviolet Light-induced Formation of Pisatin and Phenylalanine Ammonia Lyase

1971

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“…Dans la mesure ou il semble directement lit au blocage des virus et des bacttries et i l'activation des microorganismes, l'importance de l'interprttation biologique de ce phtnomkne de dimtrisation a Ctt soulignte par de nombreux auteurs (4)(5)(6)(7). En ce qui concerne la rtversibilitt de la rtaction photochimique, l'tquilibre de conversion thymine-dimkre en thymine-monomkre peut 6tre envisagt sous deux aspects compltmentaires: (i) par irradiation, i une longueur d'onde voisine de 250 nm, de la moltcule de thymine-dimkre isolte (8) la valeur concorde parfaitement avec les rtfkrences ci-dessus (tableau 1).…”

Section: Introductionunclassified

Complexes des ions Hg²⁺ avec Le monomère et le dimère de la thymine: 5-methyl-2,4 (1H, 3H) pyrimidinedione

Petit‐Ramel¹,

Thomas-David²,

Périchet³

et al. 1984

Can. J. Chem.

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M. M. PETIT-RAMEL, G. THOMAS-DAVID, G. PERICHET et P. POUYET. Can. J. Chem. 62, 22 (1984). Les constantes d'association relatives aux complexes des ions Hg2' avec les formes monomkre (TM) et dimkre (TD) de la thymine sont calculCes a partir de mesures protomCtriques effectuCes dans un large domaine de pH: 2 < pH < 11. Le traitement des donnCes de chacun des systkmes est conduit par une mtthode de pit-mapping et l'on peut donner la ripartition des diverses espkces complexes en fonction du pH.En ce qui conceme le monomkre, les ions Hg2' ayant une coordinence de degrC deux, traduite par la configuration linCaire d'espkces non chClatCes, on peut mettre en evidence les complexes [H~ (

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Strahlenchemische Ver�nderung der Bakterien-Desoxyribonucleins�ure in vivo

Cited by 182 publications

References 6 publications

Crystal structure of a DNA decamer containing a cis-syn thymine dimer

Crystal structure of a DNA decamer containing a cis-syn thymine dimer

Ultraviolet Light-induced Formation of Pisatin and Phenylalanine Ammonia Lyase

Complexes des ions Hg²⁺ avec Le monomère et le dimère de la thymine: 5-methyl-2,4 (1H, 3H) pyrimidinedione

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Strahlenchemische Ver�nderung der Bakterien-Desoxyribonucleins�ure in vivo

Cited by 182 publications

References 6 publications

Crystal structure of a DNA decamer containing a cis-syn thymine dimer

Crystal structure of a DNA decamer containing a cis-syn thymine dimer

Ultraviolet Light-induced Formation of Pisatin and Phenylalanine Ammonia Lyase

Complexes des ions Hg2+ avec Le monomère et le dimère de la thymine: 5-methyl-2,4 (1H, 3H) pyrimidinedione

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Complexes des ions Hg²⁺ avec Le monomère et le dimère de la thymine: 5-methyl-2,4 (1H, 3H) pyrimidinedione