Storage Temperatures for Cold-Chain Delivery in Cell Therapy: A Study of Alginate-Encapsulated Liver Cell Spheroids Stored at −80°C or −170°C for Up to 1 Year

Massie, Isobel; Selden, Clare; Hodgson, Humphrey; Fuller, Barry

doi:10.1089/ten.tec.2012.0307

Cited by 34 publications

(28 citation statements)

References 25 publications

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“…These findings are consistent with those reported for blood stem cells in which there were no significant differences in cell viability parameters between protocols that included storage in liquid nitrogen or freezing at -80°C (McCullough et al 2010). Overall, although some changes undoubtedly occur during cell storage (Massie et al 2013), our results indicate that storage of Chaoborus larvae for 1 year at -80°C is an effective means of preserving these insects without substantially altering the subcellular distributions of the metals.…”

Section: Efficacy Of Preservation Conditionssupporting

confidence: 91%

Assessment of a subcellular metal partitioning protocol for aquatic invertebrates: preservation, homogenization, and subcellular fractionation

Rosabal

Hare

Campbell

2014

Limnology & Ocean Methods

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Measurements of the subcellular partitioning of trace metals have been used to predict assimilation efficiencies for metals between trophic levels and to evaluate metal detoxification mechanisms in aquatic organisms. Using field-collected larvae of the insect Chaoborus, we assessed the performance of two preservation protocols, three homogenization treatments, and a cell fractionation protocol to determine the best combination of techniques for assessing the subcellular partitioning of Cd, Cu, Ni, and Zn. Preservation of larval samples for either 2 years at -80°C or 1 year at -80°C or -196°C had little influence on the subcellular partitioning of these metals. Using several marker enzymes, i.e., cytochrome C oxidase and citrate synthase for mitochondria, β-hexosaminidase for lysosomes, and lactate dehydrogenase for the cytosol, we show that optimal and high-yield homogenization is achieved using a combination of mechanical disruption using a micro-pestle followed by vortexing and that the subsequent fractionation procedure employing differential centrifugation/NaOH digestion was effective. We conclude that the preservation and homogenization protocols as well as the fractionation procedure that we tested using Chaoborus larvae can serve as a model approach for improving current protocols and for designing protocols for previously untested biological samples.

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Section: Efficacy Of Preservation Conditionssupporting

confidence: 91%

Assessment of a subcellular metal partitioning protocol for aquatic invertebrates: preservation, homogenization, and subcellular fractionation

Rosabal

Hare

Campbell

2014

Limnology & Ocean Methods

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“…In contrast, liver cell spheroids stored for 12 months at -170°C maintained viability. 95 These examples for the storage of cellular samples show that some cell types were sucessfully stored at -80°C and retained their viability over time. However, other cell types were found to lose their viability even after short periods of time at -80°C and thus require lower storage temperatures (e.g., -170°C).…”

Section: Storage Of Cryopreserved Cellsmentioning

confidence: 99%

“…Most of the quality tests revealed no significant differences; only the total nucleated cell count for samples stored for 24 hours at 1°C-6°C before cryopreservation declined over time. 94 Massie et al 95 investigated the effects of storage temperature on viability of alginate-encapsulated liver cell spheroids. Spheroids were stored at either -80°C or * -170°C for up to 12 months.…”

Section: Storage Of Cryopreserved Cellsmentioning

confidence: 99%

Storage of Human Biospecimens: Selection of the Optimal Storage Temperature

Hubel

Spindler

Skubitz

2014

Biopreservation and Biobanking

119

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Millions of biological samples are currently kept at low tempertures in cryobanks/biorepositories for long-term storage. The quality of the biospecimen when thawed, however, is not only determined by processing of the biospecimen but the storage conditions as well. The overall objective of this article is to describe the scientific basis for selecting a storage temperature for a biospecimen based on current scientific understanding. To that end, this article reviews some physical basics of the temperature, nucleation, and ice crystal growth present in biological samples stored at low temperatures (-20°C to -196°C), and our current understanding of the role of temperature on the activity of degradative molecules present in biospecimens. The scientific literature relevant to the stability of specific biomarkers in human fluid, cell, and tissue biospecimens is also summarized for the range of temperatures between -20°C to -196°C. These studies demonstrate the importance of storage temperature on the stability of critical biomarkers for fluid, cell, and tissue biospecimens.

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“…In comparison to storage below –135°C, it has been shown to be inferior for long-term storage of both cells and tissues [59, 60]. Significant deterioration in viability has been noted in peripheral blood mononuclear cells after 14 months of storage at –80°C [61] and in PBSCs, where clonogenic capacity was lost over periods of as little as 5 months [62].…”

Section: Storage and Transportationmentioning

confidence: 99%

Technical Considerations in the Freezing, Low-Temperature Storage and Thawing of Stem Cells for Cellular Therapies

Hunt¹

2019

Transfus Med Hemother

100

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The commercial and clinical development of cellular therapy products will invariably require cryopreservation and frozen storage of cellular starting materials, intermediates and/or final product. Optimising cryopreservation is as important as optimisation of the cell culture process in obtaining maximum yield and a consistent end-product. Suboptimal cryopreservation can lead not only to batch-to-batch variation, lowered cellular functionality and reduced cell yield, but also to the potential selection of subpopulations with genetic or epigenetic characteristics divergent from the original cell line. Regulatory requirements also impact on cryopreservation as these will require a robust and reproducible approach to the freezing, storage and thawing of the product. This requires attention to all aspects of the application of low temperatures: from the choice of freezing container and cryoprotectant, the cooling rate employed and its mode of delivery, the correct handling of the frozen material during storage and transportation, to the eventual thawing of the product by the end-user. Each of these influences all of the others to a greater or lesser extent and none should be ignored. This paper seeks to provide practical insights and alternative solutions to the technical challenges faced during cryopreservation of cells for use in cellular therapies.

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Storage Temperatures for Cold-Chain Delivery in Cell Therapy: A Study of Alginate-Encapsulated Liver Cell Spheroids Stored at −80°C or −170°C for Up to 1 Year

Cited by 34 publications

References 25 publications

Assessment of a subcellular metal partitioning protocol for aquatic invertebrates: preservation, homogenization, and subcellular fractionation

Assessment of a subcellular metal partitioning protocol for aquatic invertebrates: preservation, homogenization, and subcellular fractionation

Storage of Human Biospecimens: Selection of the Optimal Storage Temperature

Technical Considerations in the Freezing, Low-Temperature Storage and Thawing of Stem Cells for Cellular Therapies

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