Storage stability of water-insoluble enzymes covalently coupled to organic and inorganic carriers

Weetall, Howard H.

doi:10.1016/0005-2744(70)90171-3

Cited by 182 publications

(44 citation statements)

References 13 publications

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“…i37 Sections Cryostat sections of unfixed brain were mounted on slides of which the surfaces were activated by the following pretreatment. Cleaned microscope slides were incubated in sealed containers containing 10% T-aminopropyl-triethoxysilane (Weetall 1970) in toluene overnight at room temperature. After washing in toluence and acetone they were air dried and reacted in 1% glutaraldehyde in water for 6 h, thoroughly rinsed in water and dried again.…”

Section: Freeze Substitutionmentioning

confidence: 99%

Modifications of S100-protein immunoreactivity in rat brain induced by tissue preparation

Rickmann¹,

Wolff²

1995

Histochem Cell Biol

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Immunocytochemistry using antibodies against various molecular forms of the Ca++ and Zn(++)-binding S100 proteins predominantly labelled astrocytes. However, especially in the neocortex the staining pattern is variable. Methods of tissue preparation have been evaluated with the aim to preserve as much S100 immunoreactivity as possible. Optimal results were obtained after perfusion fixation with 4-5% aldehydes, 0.1 M sodium cacodylate, 0.1% CaCl2, pH 7.3. In such preparations, astrocytes were completely labelled including their lamellar compartments in large parts of the central nervous system. Ca(++)-withdrawal had adverse affects on S100 immunoreactivity. Cryostat sections treated with EDTA-containing solutions before fixation showed that Ca(++)-free S100 can apparently not be fixed to the tissue. Perfusion fixatives containing EDTA resulted in inhomogeneous loss of S100 staining, indicating a differential susceptibility of astrocytic subpopulations. A different type of reduction in S100 immunoreactivity occurred around large neocortical blood vessels. Perivascular defects in immunostaining occasionally appeared even after optimal fixation, but could be regularly provoked by mildly acidic fixation (pH 6.6) or prolonged barbiturate anaesthesia. These defects might be based on S100 release into the cerebrospinal fluid. Presumably under none of the conditions studied can the immunoreactivity of all S100-forms and -fractions be completely preserved in the tissue. However, recommendations are presented for optimizing tissue preparation, to the extent that premortal modifications affecting the stainability of astrocytes may be detected by S100 immunohistochemistry in fixed brain tissue.

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Section: Freeze Substitutionmentioning

confidence: 99%

Modifications of S100-protein immunoreactivity in rat brain induced by tissue preparation

Rickmann¹,

Wolff²

1995

Histochem Cell Biol

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“…The cultured dishes contained I ml of 'plating' solution (710% L-15, GIBCO Laboratories, USA; 1 % penicillin-streptomycin, Sigma, USA; 10 mm-HEPES; adjusted to pH 7-6 with NaOH, Lasater & Dowling, 1982). The bottom of each dish contained a cover-slip modified according to Weetall (1970) by the covalent attachment of concanavalin A (Boehringer Mannheim, USA). The plating solution was replaced after 2 h with a similar solution to which was added 43 mM-glucose and 1 % Nutridoma (Boehringer Mannheim, USA).…”

Section: Introductionmentioning

confidence: 99%

Modulation of an electrical synapse between solitary pairs of catfish horizontal cells by dopamine and second messengers.

DeVries

Schwartz

1989

The Journal of Physiology

264

201

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SUMMARY1. Retinas from channel catfish were dissociated and the cells maintained in culture. Horizontal cells that normally receive input from cone photoreceptors were identified. The conductance of the electrical junction formed between a pair of 'cone' horizontal cells was measured by controlling the membrane voltage of each cell with a voltage clamp maintained through either a micropipette or a patch pipette. The two techniques yielded similar results.2. Transjunctional current was measured while transjunctional voltage was stepped to values between+60 mV. The current (measured 5 ms after a step) was proportional to voltage over the range tested. For steps to voltages greater than + 45 mV, the current exhibited a slight time-dependent decline.3. Dopamine decreased junctional conductance in a dose-dependent fashion. A 50% reduction was obtained with 10 nM-dopamine. The D1 agonist fenoldopam (100 nM) also decreased junctional conductance. The uncoupling produced by either agent was rapid and reversible.

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“…First of all, the preparative processes involved, which are quite similar to sol-gel process, require simple room temperature conditions, can be prepared at a desired size, shape and porosity, and are extremely stable [31,32]. In addition, there is no swelling or porosity changes with change in pH, and these particles are not vulnerable to microbial attack [33]. These particles also effectively protect doped molecules (enzymes, drugs etc.)…”

Section: Nanoparticlesmentioning

confidence: 99%

The Therapeutic Potential of Microencapsulate Implants: Patents and Clinical Trials

Spuch¹,

Navarro

2010

EMI

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Cell microencapsulation holds promise for the treatment of many diseases by the continuous delivery of therapeutic products. The complexity of many diseases, such as neurological and metabolic disorders needs the generation of new drugs and especially new pathways to deliver the drug at proper concentration and into the correct localization. One hopeful technology is the developing of new biomaterial components with the capacity to envelope drugs, cells or tissues, being able to distribute the therapy; and at the same time, to be isolated from the immune system. Microcapsules technology has this capacity: releasing the necessary drug in a precise location with a long-term efficacy, and keeping the grafts isolated from the immune system. The objective of this review is to summarize the last patents and news related with microencapsulation technology, and the possible therapeutic applications in different disorders.

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Storage stability of water-insoluble enzymes covalently coupled to organic and inorganic carriers

Cited by 182 publications

References 13 publications

Modifications of S100-protein immunoreactivity in rat brain induced by tissue preparation

Modifications of S100-protein immunoreactivity in rat brain induced by tissue preparation

Modulation of an electrical synapse between solitary pairs of catfish horizontal cells by dopamine and second messengers.

The Therapeutic Potential of Microencapsulate Implants: Patents and Clinical Trials

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