Storage stability of soy protein isolate films incorporated with mango kernel extract at different temperature

Abstract

This research investigated the storage stability of antioxidant films made from waste and byproducts which are soy protein isolate (SPI) and mango kernel extract (MKE) stored at room temperature (25 °C), refrigeration temperature (4 °C) and frozen temperature (−18 °C) for 90 days. The thickness of the films was maintained from 0.050 to 0.058 mm until the 90th day. The colour properties of SPI films incorporated with MKE (SPI + MKE) were generally not significantly affected by time and temperature except for the b value. All the films turned darker over the storage time. There was no dominant factor between temperature and time for the mechanical properties; all the films showed an increase in tensile strength and Young's modulus, and a decrease in elongation. The antioxidant activity of the films was determined by the total phenolic content and radical scavenging activity of DPPH and ABTS. SPI + MKE film at 25 °C showed the highest antioxidant activity as compared to films stored at 4 °C and −18 °C in all the analyses, with the result being significant in DPPH and ABTS analyses. The film stored at 25 °C showed 26 to 50% higher (p > 0.05) TPC than films stored at 4 °C and −18 °C, respectively and had the highest antioxidant activity (54%) in ABTS analysis (p < 0.05). SPI + MKE film stored at 25 °C also showed only 1% depreciation of radical scavenging activity (RSA) throughout the storage time. The highest decrease (4%) in antioxidant activity was recorded for SPI + MKE film stored at −18 °C, although it was considered very low. This shows that the antioxidant activity of the films is stable for 90 days of storage.

Changes in Properties of Soy Protein Isolate Edible Films Stored at Different Temperatures: Studies on Water and Glycerol Migration

“…The results are the same as those of the whey protein film microstructure reported by Anker that when using glycerol as the plasticizer, the films presented larger pores [34]. This phenomenon indicated that glycerol as a small molecule was easily affected by storage condition changes [10], which led to a larger gap between SPI molecules, making the originally dense structure porous and fragile. Therefore, it is possible to create pores during storage [11].…”

“…Soy protein isolate (SPI) has been widely studied due to its good film-forming property [9]. Its protein content can reach more than 90%, with low cost and biodegradable superiorities [10], while strong interactions between SPI molecules lead to serious disadvantages in the film-forming process [11]. Pure SPI film without plasticizer is extremely brittle, resulting in less effective applications, mainly because the bonds formed between SPI protein chains are hydrophobic and hydrogen bonds, intermolecular disulfide bonding, and electrostatic forces [12].…”

Bioactive Compounds in Extracts from the Agro-Industrial Waste of Mango

“…At 25 °C, there was an increase in the protein–protein interactions, and the antioxidant film activity involved migration of antioxidants from the film to the product. The higher temperature promoted a higher re-arrangement process in the protein matrix, and at lower temperatures, the re-arrangement process decreased [ 81 ].…”

Stability of zein‐based films and their mechanism of change during storage at different temperatures and relative humidity

“…However, previous researches about zein films were mainly focused on the mechanical properties and barrier properties, but paid little attention to their stability. Results from a limited number of studies on the storage stability of protein film showed that storage conditions had significant influences on the mechanical properties, and protein aggregation, thiol oxidation, moisture loss were observed during film storage (Blanco-Pascual, Fernández-Martín, & Montero, 2014;Ciannamea, Stefani, & Ruseckaite, 2015;Maryam Adilaha & Nur Hanani, 2019;Orliac, Rouilly, Silvestre, & Rigal, 2003;Osés, Fernandez-Pan, Mendoza, & Maté, 2009;Piccirilli, Soazo, Pérez, Delorenzi, & Verdini, 2019). We have observed the change difference between the mechanical properties when zein films were exposed to the natural environment (uncontrolled, at ambient temperature, and humidity) in spring, summer, autumn, and winter for 60 days, respectively, with minimum change occurring in spring and maximum change in summer (Guo & Cui, 2015).…”