Storage stability of freeze–dried Lactobacillus acidophilus (La-5) in relation to water activity and presence of oxygen and ascorbate

Kurtmann, Lone; Carlsen, Charlotte U.; Risbo, Jens; Skibsted, Leif H.

doi:10.1016/j.cryobiol.2008.12.001

Cited by 76 publications

(55 citation statements)

References 28 publications

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“…The protective effect of ascorbic acid had been demonstrated on Lactobacillus bulgaricus during storage. It had been observed that the presence of ascorbate could reduce the detrimental effect of atmospheric oxygen on Lactobacillus acidophilus (Kurtmann et al, 2009). …”

Section: Discussionmentioning

confidence: 99%

Optimization of cryoprotectants for Streptococcus thermophilus during freeze-drying using Box-Behnken experimental design

Zhang

Hui

Chen

et al. 2017

Emir. J. Food Agric

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Section: Discussionmentioning

confidence: 99%

Optimization of cryoprotectants for Streptococcus thermophilus during freeze-drying using Box-Behnken experimental design

Zhang

Hui

Chen

et al. 2017

Emir. J. Food Agric

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“…The most promising technology in this regard is freeze-drying, due to milder conditions of this process that helps to prevent excessive losses of cell viability (Tsen, Chen and King, 2002;Kurtmann, Carlsen, Risbo and Skibsted, 2009;Semyonov et al, 2010;Martin-Dejardin et al, 2013).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Evaluation of pea protein–polysaccharide matrices for encapsulation of acid-sensitive bacteria

Varankovich

Khan

Nickerson

et al. 2015

Food Research International

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“…Streit et al (2007) reported a positive effect on the viability of frozen Lactobacillus bulgaricus CFL1 when pre-acidifying the fermented broth. The survival of lyophilized Lactobacillus acidophilus improved when exposed to lower water activity and oxygen level below 4% before freezing (Kurtmann et al 2009). …”

Section: Introductionmentioning

confidence: 97%

Effects of fermentation substrates and conservation methods on the viability and antimicrobial activity of Weissella confusa and its metabolites

Serna-Cock¹,

Enriquez-Valencia²,

Jiménez-Obando³

et al. 2012

Electron. J. Biotechnol.

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Lactic acid bacteria produce metabolites with antagonistic activity against other bacteria. However, growth conditions and conservation methods may reduce the viability and antimicrobial activity of lactic acid bacteria. This study evaluated the effects of fermentation substrate, lyophilization (freeze-drying) and refrigeration on the viability and antimicrobial activity of Weissella confusa strain and its metabolites against pathogens responsible for bovine mastitis. W. confusa strain was grown in MRS broth and milk supplemented with yeast extract and glucose (MYEG). The collected fractions were preserved by lyophilization or under refrigeration at 4ºC. Every seven days, the viability of W. confusa strain and the stability of its metabolites were evaluated against Staphylococcus aureus and Streptococcus agalactiae by disc diffusion assays. In both fermentation substrates, the combination of lyophilized strain and metabolites retained antimicrobial activity against the two pathogens for 42 days. Also, W. confusa strain retained adequate viability and antimicrobial activity when grown in MYEG and stored under refrigeration conditions. It was concluded that MYEG and refrigeration are acceptable low cost options to preserve the viability of W. confusa for its potential commercial use in the prevention and treatment of bovine mastitis.

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Storage stability of freeze–dried Lactobacillus acidophilus (La-5) in relation to water activity and presence of oxygen and ascorbate

Cited by 76 publications

References 28 publications

Optimization of cryoprotectants for Streptococcus thermophilus during freeze-drying using Box-Behnken experimental design

Optimization of cryoprotectants for Streptococcus thermophilus during freeze-drying using Box-Behnken experimental design

Evaluation of pea protein–polysaccharide matrices for encapsulation of acid-sensitive bacteria

Effects of fermentation substrates and conservation methods on the viability and antimicrobial activity of Weissella confusa and its metabolites

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