Storage proteins in vespid wasps: characterization, developmental pattern, and occurrence in adults

Hunt, James H.; Buck, N. A.; Wheeler, Diana E.

doi:10.1016/s0022-1910(03)00115-x

Cited by 66 publications

(56 citation statements)

References 24 publications

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“…4). The overall decline in storage protein levels from the larval stage until adult emergence reflects the pattern typical of holometabolous insects (35). Concentrations are high at the end of larval growth and then decline during the pupal stage.…”

Section: Resultsmentioning

confidence: 72%

“…Also, it is unlikely that the quantitative protein pattern (Fig. 4) is distorted by sequestration of storage protein in fat body (40), because the signatures identified for hemolymph coincide with partial patterns obtained from wholetissue preparations (35). Because the identified peptides are encoded by several different Contigs (SI Table 6), it seems likely that we identified more than one hexamerin.…”

Section: Resultsmentioning

confidence: 88%

“…35. Densitometrical analysis was performed by Quantity One imaging software (Bio-Rad, Hercules, CA) after staining the gels with Commassie Brilliant Blue (Sigma-Aldrich).…”

Section: Methodsmentioning

confidence: 99%

“…P. exclamans gynes survived a cold test at 4.5-fold worker survival (32), and delayed reproduction is the hallmark of gynes, which do not reproduce until colony initiation the next year (33). Abundant fat has been used to distinguish gynes from workers of P. exclamans (32,34), and storage proteins have been found in gynes but not workers of several Polistes species (35). These characteristics show clear correspondence to nondiapause and adult diapause (18) phenotypes of insects.…”

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confidence: 99%

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A diapause pathway underlies the gyne phenotype inPolisteswasps, revealing an evolutionary route to caste-containing insect societies

Hunt

Kensinger

Kossuth

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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142

125

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Colonies of social wasps, ants, and bees are characterized by the production of two phenotypes of female offspring, workers that remain at their natal nest and nonworkers that are potential colony reproductives of the next generation. The phenotype difference includes morphology and is fixed during larval development in ants, honey bees, and some social wasps, all of which represent an advanced state of sociality. Paper wasps (Polistes) lack morphological castes and are thought to more closely resemble an ancestral state of sociality wherein the phenotype difference between workers and nonworkers is established only during adult life. We address an alternative hypothesis: a bias toward the potential reproductive (gyne) phenotype among Polistes female offspring occurs during larval development and is based on a facultatively expressed ancestral life history trait: diapause. We show that two signatures of diapause (extended maturation time and enhanced synthesis and sequestration of a hexameric storage protein) characterize the development of gyne offspring in Polistes metricus. Hexameric storage proteins are implicated in silencing juvenile hormone signaling, which is a prerequisite for diapause. Diverging hexamerin protein dynamics driven by changes in larval provisioning levels thereby provide one possible mechanism that can cause an adaptive shift in phenotype bias during the Polistes colony cycle. This ontogenetic basis for alternative female phenotypes in Polistes challenges the view that workers and gynes represent behavior options equally available to every female offspring, and it exemplifies how social insect castes can evolve from casteless lineages.caste development ͉ hexameric storage proteins ͉ juvenile hormone ͉ life-history pleiotropy ͉ social behavior S ocial insects often show pronounced differences in behavior, physiology, and morphology between castes of workers (functionally sterile helpers) and reproductives (1).

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Section: Resultsmentioning

confidence: 72%

Section: Resultsmentioning

confidence: 88%

“…35. Densitometrical analysis was performed by Quantity One imaging software (Bio-Rad, Hercules, CA) after staining the gels with Commassie Brilliant Blue (Sigma-Aldrich).…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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A diapause pathway underlies the gyne phenotype inPolisteswasps, revealing an evolutionary route to caste-containing insect societies

Hunt

Kensinger

Kossuth

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

142

125

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“…New M. mastigophorus colonies are initiated throughout the year in the Monteverde area (O'Donnell and Joyce, 2001). Therefore, a newly emerged female's reproductive caste status is not seasonally constrained, as it is for many temperate paper wasp species (O'Donnell, 1998b;Hunt et al, 2003). Furthermore, females' task performance patterns depend on their dominance status, and their behavior can change in response to changes in colony social structure (O'Donnell, 1998c).…”

Section: Discussionmentioning

confidence: 99%

Developmental and dominance‐associated differences in mushroom body structure in the paper wasp Mischocyttarus mastigophorus

O’Donnell

Donlan

Jones

2006

Developmental Neurobiology

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Primitively eusocial paper wasps exhibit considerable plasticity in their division of labor. Dominance interactions among nest mates play a strong role in determining the task performance patterns of adult females. We asked whether dominance status and task performance differences were associated with the development of subregions of the mushroom bodies (MB) of female Mischocyttarus mastigophorus queens and workers. We found that the MB calycal neuropils were better developed (relative to the Kenyon cell body layer) in the dominant females that spent more time on the nest. Increased MB calyx development was more strongly associated with social dominance than with high rates of foraging. The MB of queens resembled those of dominant workers. The results suggest that social interactions are particularly relevant to M. mastigophorus females' cognition. By examining the MB of newly emerged females, we also found evidence for significant age-related changes in MB structure.

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Characterization and expression of the Hex 110 gene encoding a glutamine‐rich hexamerin in the honey bee, Apis mellifera

Bitondi

Nascimento

Cunha

et al. 2006

Arch Insect Biochem Physiol

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An N-terminal amino acid sequence of a previously reported honey bee hexamerin, HEX 110 [Danty et al., Insect Biochem Mol Biol 28:387-397 (1998)], was used as reference to identify the predicted genomic sequence in a public GenBank database. In silico analysis revealed an ORF of 3,033 nucleotides that encompasses eight exons. The conceptual translation product is a glutamine-rich polypeptide with a predicted molecular mass of 112.2 kDa and pI of 6.43, which contains the conserved M and C hemocyanin domains. Semiquantitative and quantitative RT-PCR with specific primers allowed for an analysis of mRNA levels during worker bee development and under different physiological conditions. Concomitantly, the abundance of the respective polypeptide in the hemolymph was examined by SDS-PAGE. Hex 110 transcripts were found in high levels during the larval stages, then decreased gradually during the pupal stage, and increased again in adults. HEX 110 subunits were highly abundant in larval hemolymph, decreased at the spinning-stage, and remained at low levels in pupae and adults. In 5th instar larvae, neither starvation nor supplementation of larval food with royal jelly changed the Hex 110 transcript levels or the amounts of HEX 110 subunit in hemolymph. In adult workers, high levels of Hex 110 mRNA, but not of the respective subunit, were related to ovary activation, and also to the consumption of a pollen-rich diet.

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Storage proteins in vespid wasps: characterization, developmental pattern, and occurrence in adults

Cited by 66 publications

References 24 publications

A diapause pathway underlies the gyne phenotype inPolisteswasps, revealing an evolutionary route to caste-containing insect societies

A diapause pathway underlies the gyne phenotype inPolisteswasps, revealing an evolutionary route to caste-containing insect societies

Developmental and dominance‐associated differences in mushroom body structure in the paper wasp Mischocyttarus mastigophorus

Characterization and expression of the Hex 110 gene encoding a glutamine‐rich hexamerin in the honey bee, Apis mellifera

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