Storage of Yarrowia lipolytica lipase after spray-drying in the presence of additives

Alloue, Wazé Aimée Mireille; Destain, Jacqueline; Amighi, Karim; Thonart, Philippe

doi:10.1016/j.procbio.2007.05.024

Cited by 39 publications

(26 citation statements)

References 22 publications

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“…Alloue et al (2007) showed the powders produced by spray drying supplemented with maltodextrin or gum arabic, both with and without calcium chloride, were also stored for 12 months at 4 or 20 °C without loss of activities. In all cases, additives used to stabilize lipase allowed for ADJ= drying adjuvant; Ws= feed flow rate; TGI= inlet gas temperature; Tgo= outlet gas temperature; RAE= retention of the enzymatic activity; REC= product recovery; XP= moisture content; Aw=water activity; *with 0.1% Tween 80.…”

Section: Spray Drying Stabilization Of Purified Lipasementioning

confidence: 99%

Characterization and spray drying of lipase produced by the endophytic fungus Cercospora kikuchii

Costa-Silva

Souza

Oliveira

et al. 2014

Braz. J. Chem. Eng.

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-A lipase from the endophytic fungus Cercospora kikuchii was purified, biochemically characterized and the effects of spray drying on stabilization of the purified enzyme were studied. The lipase was purified 9.31-fold with recovery of 26.6% and specific activity of 223.6 U/mg. The optimum pH and temperature were 4.6 and 35 °C, respectively, while the Vmax was 10.28 µmol/min.mg -1 protein and Km 0.0324 mM. All the metal ions tested enhanced the enzyme activity. The lipase retained almost 100% activity in the presence of strong oxidants and was also resistant to Triton X, Tween 80 and 20 and SDS, as well as to proteases. The purified lipase was spray dried and kept until 85.2% of enzymatic activity. At least 70% of the enzymatic activity was maintained for spray dried purified lipase during the storage period. The lipase produced by Cercospora kikuchii has properties useful for industrial application and showed adequate stabilization and retention of its enzymatic activity after spray drying.

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Section: Spray Drying Stabilization Of Purified Lipasementioning

confidence: 99%

Characterization and spray drying of lipase produced by the endophytic fungus Cercospora kikuchii

Costa-Silva

Souza

Oliveira

et al. 2014

Braz. J. Chem. Eng.

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“…Above 70°C skimmed milk was not eVective in retaining the thermo stability. Addition of 6-12% milk powder, a source of casein and calcium ions, was found to retain 76-82% of lipase activity when added to fermented broth before spray drying [1,6]. Use of antimicrobial agents and sodium chloride has been reported to avoid contamination in fermented broth and stabilization of alkaline protease activity from Conidiobolous coronatus [14].…”

Section: Stability Of Phytase In Liquid and Solid Conditionsmentioning

confidence: 99%

Strain improvement and up scaling of phytase production by Aspergillus niger NCIM 563 under submerged fermentation conditions

Shah

Bhavsar

Soni

et al. 2008

J Ind Microbiol Biotechnol

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Combination of physical and chemical mutagenesis was used to isolate hyper secretory strains of Aspergillus niger NCIM 563 for phytase production. Phytase activity of mutant N-1 and N-79 was about 17 and 47% higher than the parent strain. In shake flask the productivity of phytase in parent, mutant N-1 and N-79 was 6,181, 7,619 and 9,523 IU/L per day, respectively. Up scaling of the fermentation from shake flask to 3 and 14 L New Brunswick fermenter was studied. After optimizing various fermentation parameters like aeration, agitation and carbon source in fermentation medium the fermentation time to achieve highest phytase activity was reduced considerably from 14 days in shake flask to 8 days in 14 L fermenter. Highest phytase activity of 80 IU/ml was obtained in 1% rice bran-3.5% glucose containing medium with aeration 0.2 vvm and agitation 550 rpm at room temperature on 8th day of fermentation. Addition of either bavistin (0.1%), penicillin (0.1%), formalin (0.2%) and sodium chloride (10%) in fermented broth were effective in retaining 100% phytase activity for 8 days at room temperature while these reagents along with methanol (50%) and ethanol (50%) confer 100% stability of phytase activity at 4 degrees C till 20 days. Among various carriers used for application of phytase in feed, wheat bran and rice bran were superior to silica and calcium carbonate. Thermo stabilization studies indicate 100% protection of phytase activity in presence of 12% skim milk at 70 degrees C, which will be useful for its spray drying.

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“…It is however usually associated with several problems, including the large particle size, the wide particle size distribution and the low specific surface area. On the other hand, spray drying, as a simple, fast, and economical technique to obtain a powder from a solution or a liquid suspension [13,14] , has been shown to be a promising method in polymeric absorbent preparation. The advantages include the relatively narrow particle size distribution, the one step preparation of particles with the required size, the simple fabricating process and the flexibility [15][16][17][18] .…”

Section: Introductionmentioning

confidence: 99%

Preparation of Highly Oil-Absorbing Resins Consisting of Hollow Nanoparticle Aggregates by Spray Drying

Huang¹,

Xiao²,

Chen³

et al. 2009

Polymer-Plastics Technology and Engineering

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We report on the preparation of a novel poly(styrene-dodecyl methacrylate) (P(St-DMA)) absorbent resin by spray drying. The microstructures of the prepared resins have been examined by scanning electron microscope (SEM), showing that the resins consisted of hollow micrometer-sized spheres that were aggregated by nanoparticles. Meanwhile, the effects of the content of the cross-linker divinybenzene (DVB) on the oil absorption capacity, the desorption efficiency and the resin microstructures have also been studied. The absorption performance and the hydrophobic properties of the resins prepared by spray drying and vacuum drying have been studied and compared.

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Storage of Yarrowia lipolytica lipase after spray-drying in the presence of additives

Cited by 39 publications

References 22 publications

Characterization and spray drying of lipase produced by the endophytic fungus Cercospora kikuchii

Characterization and spray drying of lipase produced by the endophytic fungus Cercospora kikuchii

Strain improvement and up scaling of phytase production by Aspergillus niger NCIM 563 under submerged fermentation conditions

Preparation of Highly Oil-Absorbing Resins Consisting of Hollow Nanoparticle Aggregates by Spray Drying

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