Storage of ram semen

Salamon, S.; Maxwell, W.M.C.

doi:10.1016/s0378-4320(00)00155-x

Cited by 553 publications

(485 citation statements)

References 69 publications

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“…This difference with our study could be because of conventional static vapor freezing method used by Rao et al (1986). Therefore, it appears that spermatozoa thawed at a fast rate may also be exposed for a shorter time to the concentrated solute and cryoprotectant-glycerol, and the restoration of the intracellular and extracellular equilibrium is more rapid than for slow thawing (Salamon & Maxwell, 2000). Contrary to above, higher sperm DNA-I was reported with T1 (95.56%) compared to T2 (93.83%) and T3 (93.93%).…”

Section: Discussionmentioning

confidence: 75%

“…Regarding equilibration, it is traditionally called the 'glycerol equilibration' period, during which spermatozoa interact with glycerol before freezing. During this stage, glycerol rapidly penetrates into the spermatozoa to establish equilibrium between its intracellular and extracellular concentration and other osmotically active extender components (Salamon & Maxwell, 2000). Therefore, this phenomenon interacts with the type of extender (buffer and cryoprotectant) used and could easily interact with other cryogenic procedures (Marshall, 1984).…”

Section: Introductionmentioning

confidence: 99%

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Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Shah

Andrabi²,

Qureshi

2016

Andrology

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SUMMARYThe effects of equilibration times (E1, 2 h; E2, 4 h; E3, 6 h), freezing rates (FR1, manual, 5 cm above liquid nitrogen (LN 2 ) for 10 min, plunging in LN 2 ; FR2, programmable ultra-fast, holding at +4°C for 2 min, from 4 to À10°C at À10°C/min, from À10 to À20°C at À15°C/min, from À20 to À120°C at À60°C/min, holding at À120°C for 30 sec, plunging in LN 2 ), and thawing rates (T1, 37°C for 30 sec; T2, 50°C for 15 sec; T3, 70°C for 7 sec) were evaluated on quality of buffalo bull spermatozoa. Progressive motility (%), rapid velocity (%), average path velocity (VAP, lm/s), straight line velocity (VSL, lm/s), and mitochondrial transmembrane potential (%) were higher (p < 0.05) with E2, FR2, and T3 compared to other groups. Sperm curved line velocity (VCL, lm/s) was higher (p < 0.05) with E2 and FR2 compared to other groups. Sperm straightness (%) and linearity (LIN, %) were higher (p < 0.05) with E2 compared to other groups. Sperm LIN was affected (p < 0.05) with T3 compared to other groups. Supravital-plasma membrane integrity (%), viability and acrosome integrity (%) of spermatozoa were higher (p < 0.05) with E2 and FR2 compared to other groups. Sperm DNA integrity (%) was higher (p < 0.05) with FR2 and T1 compared to other groups. We concluded that inclusion of 4 h-equilibration time, programmable ultra-fast freezing rate, and rapid thawing at 70°C for 7 sec in cryopreservation protocol improves the post-thaw quality of buffalo bull spermatozoa.

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Section: Discussionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Shah

Andrabi²,

Qureshi

2016

Andrology

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“…Considering that these parameters are reputed as fertility indicators (Amann et al, 2000;Farrell et al, 2000;Kathiravan et al, 2008), if cryopreservation is not immediately feasible, it becomes essential to prevent the loss of speed and linearity of epididymal sperm cells through the testicle refrigeration over time. The refrigeration temperature seems to prevent the decay of epididymal sperm quality over time, in particular in sperm motility, inducing a reduction in the metabolism of sperm cells, in terms of mitochondrial oxidative phosphorylation and glycolytic activities (Salamon and Maxweel, 2000).…”

Section: Discussionmentioning

confidence: 99%

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Turri

Madeddu

Gliozzi

et al. 2014

Animal

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The interest to develop assisted reproductive technologies and cryobanking for farm animal genetic resource conservation has recently increased. However, cryopreservation for ex-situ management of genetic diversity sometimes is not routinely feasible, owing to the lack of facilities (AI centres, laboratories) and expertise near the local breed farming area. In these cases, epididymal sperm obtained from slaughtered or castrated animals, associated with the possibility of managing rather long periods between animal death, sperm recovery and freezing, would increase the opportunities to create semen storages. This investigation addresses the pre-freeze/post-thaw quality of goat epididymal sperm as a function of testicle storage temperature (environment or +5°C) and time elapsed between animal's death and sperm recovery (0, 24, 48, 72 h) to establish the optimal protocols for the recovery and cryopreservation of epididymal sperm in this species. Testicles of 50 mature bucks collected at the abattoir were divided in two groups: half of the testicles (n = 50) were transported to the laboratory at environment temperature (E), whereas the remaining half (n = 50) at a refrigeration temperature (R) of +5°C. In the two groups (E) and (R), one testicle from each pair was processed after slaughter forming the time 0 groups (0E and 0R). The contralateral testicle was processed after 24, 48 or 72 h of storage, at the corresponding temperature. Sperm motility and kinetic parameters, viability and morphology were assessed in pre-freeze and post-thaw samples. Until 48 h postmortem, both E and R temperatures are able to maintain good pre-freeze epididymal sperm quality. After 48 h postmortem, R temperature is fundamental to reduce epididymal sperm quality decay in pre-freeze samples. Moreover, testicle refrigeration also has a positive impact on post-thaw samples, allowing a lower decline through time considering total motility, kinetics parameters, sperm viability and sperm abnormalities. Therefore, when sperm cryopreservation is not immediately practicable, goat testicles should be transported and stored at 5°C up to a maximum of 48 h postmortem to ensure an acceptable sperm quality.

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“…Cold shock reduces membrane permeability to water and solutes and injures acrosomal membranes (Purdy, 2006). The main changes that occurs during freezing are mainly ultrastructural, biochemical and functional, which impairs sperm transport and survival in the female reproductive tract and reduces fertility in domestic species (Salamon & Maxwell, 2006). The ultra structural damage is greater in ram than bull spermatozoa.…”

Section: Sperm Cryopreservationmentioning

confidence: 99%

“…The ultra structural damage is greater in ram than bull spermatozoa. Greater damages have been detected in plasma and acrosome membranes, mitochondrial sheath and axoneme (Salamon & Maxwell, 2006;Barbas & Mascarenhas, 2009). …”

Section: Sperm Cryopreservationmentioning

confidence: 99%

Fertility Cryopreservation

Chian¹,

Quinn²

2010

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Protecting the reproductive potential of young patients undergoing cancer therapy is increasingly important. With modern treatment protocols, 80% of patients can be expected to survive. It has been estimated that up to one in 250 young adults will be a survivor of childhood cancer in the future; infertility, however, may be a consequence. As a wide range of fertility preservation methods are increasingly offered by clinicians, this systematic and comprehensive textbook dealing with the cryobiology, technology and clinical approach to this therapy will be essential reading to infertility specialists, embryologists, oncologists, cryobiologists, ObGyns, andrologists, and urologists with an interest in fertility preservation. Fertility Cryopreservation reviews all the techniques of this increasingly important field within reproductive medicine. It covers the basic principles of pertinent cryobiology, and contains major sections on the different therapies available, written by international specialists combining experience from both academic centers and commercial industries.

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Storage of ram semen

Cited by 553 publications

References 69 publications

Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Fertility Cryopreservation

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