Storage of Porcine Cornea in an Innovative Bioreactor

Guindolet, Damien; Crouzet, Emmanuel; Hé, Zhiguo; Herbepin, Pascal; Jumelle, Clotilde; Perrache, Chantal; Dumollard, Jean Marc; Forest, Fabien; Péoc’h, Michel; Gain, Philippe; Gabison, Éric; Thuret, Gilles

doi:10.1167/iovs.17-22218

Cited by 24 publications

(21 citation statements)

References 37 publications

(46 reference statements)

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“…Consistent with our prior experience, 24 , 26 – 28 , 41 , 50 the perfused porcine anterior segments did not experience the amount of corneal edema reported with other culture techniques. 51 With intact TM, NO increased the facility by approximately 45% in group NO compared with the control group, C. After 360° AIT in experiment 2, we found an NO-mediated facility difference of approximately 60% between AIT-NO and AIT-C, in line with the findings reported by McDonnell et al 44 This difference persisted throughout experiments 1 and 2 but declined toward the end of experiment 1, possibly due to a decreasing TM facility response to NO. The TM makes for a significant contribution to outflow resistance, but not in experiment 2 where the facility enhancement is TM independent.…”

Section: Discussionsupporting

confidence: 91%

Structure–Function Changes of the Porcine Distal Outflow Tract in Response to Nitric Oxide

Waxman

Wang

Dang

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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PurposeTo correlate outflow function and outflow tract vessel diameter changes induced by nitric oxide (NO).MethodsIn a porcine anterior segment perfusion model, the effects of a nitric oxide donor (100 μM DETA-NO) on outflow facility were compared with controls (n = 8 per group) with trabecular meshwork (TM) and after circumferential ab interno trabeculectomy (AIT). Outflow structures were assessed with spectral-domain optical coherence tomography (SD-OCT) before and after NO, or an NO synthase inhibitor (100 μM L-NAME) and the vasoconstrictor, endothelin-1 (100 pg/mL ET-1). Scans were processed with a custom macroscript and aligned for automated reslicing and quantification of cross-sectional outflow tract areas (CSA).ResultsThe facility increased after DETA-NO (Δ of 0.189 ± 0.081 μL/min·mm Hg, P = 0.034) and AIT (Δ of 0.251 ± 0.094 μL/min·mm Hg, P = 0.009), respectively. Even after AIT, DETA-NO increased the facility by 61.5% (Δ of 0.190 ± 0.074 μL/min·mm Hg, P = 0.023) and CSA by 13.9% (P < 0.001). L-NAME + ET-1 decreased CSA by −8.6% (P < 0.001). NO increased the diameter of focal constrictions 5.0 ± 3.8-fold.ConclusionsNO can dilate vessels of the distal outflow tract and increase outflow facility in a TM-independent fashion. There are short, focally constricting vessel sections that display large diameter changes and may have a substantial impact on outflow.

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Section: Discussionsupporting

confidence: 91%

Structure–Function Changes of the Porcine Distal Outflow Tract in Response to Nitric Oxide

Waxman

Wang

Dang

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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“…Consistent with our prior experience, 21,23,24,34,41,42 the perfused porcine anterior segments did not experience the amount of corneal edema reported with other culture techniques. 43 With intact TM, nitric oxide increased the facility by approximately 45% in group NO compared to the control group, C. After 360° AIT in experiment 2, we found a facility difference of approximately 60% between AIT-NO and AIT-C, in line with the findings reported by McDonnell et al 37 This difference persisted throughout experiments 1 and 2 but declined toward the end of experiment 1, possibly due to a decreasing TM facility response to NO. The TM makes for a significant contribution to outflow resistance, but not in experiment 2 where the facility enhancement is TM-independent.…”

Section: Discussionsupporting

confidence: 91%

Structure-Function Changes of the Distal Outflow Tract in Response to Nitric Oxide

Waxman¹,

Wang²,

Dang³

et al. 2018

Preprint

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Purpose: To correlate outflow function and outflow tract vessel diameter changes induced by nitric oxide (NO).Methods: In a porcine anterior segment perfusion model, the effects of a nitric oxide donor (100 µM DETA-NO) on outflow facility were compared to controls (n=8 per group) with trabecular meshwork (TM) and after circumferential ab interno trabeculectomy (AIT). Outflow structures were assessed with spectral domain optical coherence tomography (SD-OCT) before and after NO, or an NO synthase inhibitor (100 µM L-NAME) and the vasoconstrictor, endothelin-1 (100 pg/mL ET-1). Scans were processed with a custom macro script and aligned for automated reslicing and quantification of cross-sectional outflow tract areas (CSA). Results:The facility increased after DETA-NO (0.189±0.081 μL/min·mmHg, p=0.034) and AIT (0.251±0.094 μL/min·mmHg, p=0.009), respectively. Even after AIT, DETA-NO increased the facility by 61.5% (0.190±0.074 μL/min·mmHg, p=0.023) and CSA by 13.9% (p<0.001). L-NAME + ET-1 decreased CSA by -8.6% (p<0.001). NO increased the diameter of focal constrictions 5.0±3.8 fold.Conclusions: NO can dilate vessels of the distal outflow tract and increase outflow facility in a TMindependent fashion. There are short, focally constricting vessel sections that display large diameter changes and may have a substantial impact on outflow.

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“…Shortly thereafter, Mueller injected a preservation solution containing DMSO into the anterior chamber of an eyeball, placing the eyeball in a preservation solution containing glycerol. The cornea was removed before surgery for full-thickness transplantation [100,101]. In 1965, Capella [102] used DMSO as an antifreeze to improve a cryopreservation method, ensuring corneal graft activity.…”

Section: Corneal Cryopreservation Technologymentioning

confidence: 99%

Cryopreservation in Ophthalmology

Shao¹,

Chen²,

Zhou³

et al. 2020

Cryopreservation - Current Advances and Evaluations

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Amniotic membranes (AMs) and corneas are critical materials in ocular surface reconstruction. AM has specific structures (e.g., basement and two types of cells with stemness characteristics: amniotic epithelial cells and amniotic mesenchymal cells), which contribute to its attractive physical and biological properties that make it fundamental to clinical application. The corneal endothelial cell is a vital part of the cornea, which can influence postoperative vision directly. However, widespread use of fresh AM and cornea has been limited due to their short use span and safety concerns. To overcome these concerns, different preservation methods have been introduced. Cryopreservation is distinguished from many preservation methods for its attractive advantages of prolonged use span, optimally retained tissue structure, and minimized infection risk. This review will focus on recent advances of cryopreserved AM and cornea, including different cryopreservation methods and their indications in ophthalmology.

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Storage of Porcine Cornea in an Innovative Bioreactor

Abstract: By recreating equivalent IOP and medium renewal, the BR obtained unprecedented storage quality of porcine corneas and preserved their main epithelial, limbal, and endothelial characteristics.

Cited by 24 publications

References 37 publications

Structure–Function Changes of the Porcine Distal Outflow Tract in Response to Nitric Oxide

Structure–Function Changes of the Porcine Distal Outflow Tract in Response to Nitric Oxide

Structure-Function Changes of the Distal Outflow Tract in Response to Nitric Oxide

Cryopreservation in Ophthalmology

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