Storage of pooled platelet concentrates

Snyder, Edward L.; Stack, Gary; Napychank, Paula A.; Roberts, Stanley C.

doi:10.1046/j.1537-2995.1989.29589284136.x

Cited by 49 publications

(43 citation statements)

References 12 publications

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“…These results are in concordance with observations of Snyder et al [18] and Moroff et al [19]. They studied lymphoprolifér ation by measurement of 3H-thymidine uptake in pools of PC from platelet-rich plasma.…”

Section: Evaluation Of Lymphoprolifération In Stored Bc-pcsupporting

confidence: 91%

Cytokines in Platelet Concentrates Prepared from Pooled Buffy Coats

Klüter¹,

Müller‐Steinhardt²,

Danzer³

et al. 1995

Vox Sanguinis

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Platelet concentrates (PC) prepared from pooled buffy coat (BC-PC) contain a variable number of leukocytes from different donors. We questioned whether storage of BC-PC can lead to a lymphocyte activation in the sense of a mixed lymphocyte reaction. BC-PC were prepared from four ABO-identical buffy coats and we undertook leukocyte analyses and measurement of different cytokines on days 1, 3 and 5 of PC storage (n = 72). Cytokine content was also determined in freshly prepared plasma (n = 48) and PC prepared by thrombapheresis (SD-PC) (n = 12). As control, we studied lymphoproliferation of pooled peripheral blood mononuclear cells from four individuals in 10 mixed lymphocyte cultures (MLCs) under optimal conditions. In the BC-PC, whole blood count and lymphocyte analysis showed a mean leukocyte contamination of 64±28 x 10^6 per unit with a proportion of lymphocytes of 66.7±13%. In the MLC, levels of interleukin-2 (IL-2) and interferon-γ (IFN-γ) were increased on day 3 and 5 of storage (p< 0.001). In a proportion of BC-PC, tumor necrosis factor-α (72.2%) and IL-2 (43.1%) were detectable immediately after preparation, whereas IFN-γ (4.2%), interleukin-lβ (4.2%) and interleukin-8 (11.1%) were only found in some BC-PC. In all cases, initial values of cytokines did not increase during storage. Cytokine measurement in FFP and SD-PC showed similar results. The study demonstrates that cytokines are detectable in a variety of blood products immediately after preparation. Levels of cytokines did not increase in the preparations. BC-PC can be stored for up to 5 days without any signs of lymphocyte activation.

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Section: Evaluation Of Lymphoprolifération In Stored Bc-pcsupporting

confidence: 91%

Cytokines in Platelet Concentrates Prepared from Pooled Buffy Coats

Klüter¹,

Müller‐Steinhardt²,

Danzer³

et al. 1995

Vox Sanguinis

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“…This reduction in platelet counts parallels the 5.5% drop in counts observed in 17/20 LR PCs over the same period. The tendency for platelet counts of PCs recovered from whole blood to apparently increase from day 0 to day 5 has been previously documented by a number of other investigators [45, 46, 47]. …”

Section: Discussionsupporting

confidence: 57%

Prevention of Cytokine Accumulation in Platelets Obtained with the COBE Spectra Apheresis System

Palmer

Aye

Dumont³

et al. 1998

Vox Sanguinis

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Background and Objectives: Febrile nonhemolytic transfusion reactions frequently accompany platelet transfusions and may be due to accumulation of cytokines mediating inflammation during storage of platelet concentrates (PCs). We wished to determine whether PCs collected using the COBE® SpectraTM Apheresis System (Version 4) were sufficiently leukocyte reduced (LR) to limit cytokine accumulation during storage. Materials and Methods: Cytokine accumulation – interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor-α (TNF-α) – and release of platelet α-granule – P-selectin, transforming growth factor-β1 (TGF-β1), platelet-derived growth factor AB (PDGF-AB), von Willebrand factor (vWf) – or dense granule (serotonin) markers were investigated during a 7-day storage period comparing apheresis-collected, LR PCs (LR PCs) and random donor platelets prepared from whole blood (WB). Results: Leukocyte counts were reduced 99.95% comparing LR PCs (5.7 × 10⁵/l) and WB PCs (1.09 × 10⁹/l). Little or no accumulation of leukocyte-derived cytokines was observed in LR PCs during storage in contrast to WB PCs. A reduction in the release of platelet α-granule proteins, such as P-selectin, TGF-β1 and PDGF-AB, was observed on day 0 for LR PCs compared to WB PCs with little or no difference observed from day 3 to 7. Plasma vWf levels were higher in LR PCs compared to WB PCs on days 0–7. Conclusion: Leukocyte levels in PCs collected with the COBE Spectra Apheresis System are sufficiently low to limit cytokine production during 7 days of storage.

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“…Furthermore, it was observed that leukocyte-derived cytokines are not detectable in PC with a leukocyte contamination below 0.1 × 10 9 [10, 16]. Several groups investigated the risk that passenger PBMC from different individuals in pooled PC generate mixed lymphocyte reactions during storage [17, 18]. However, no evidence for the generation of activated lymphocytes or lymphoproliferation such as 3 H thymidine uptake could be found, but the reason for this remained unclear.…”

Section: Discussionmentioning

confidence: 99%

“…The strong impact of temperature and anticoagulant on cytokine release could explain why several groups failed to detect signs of lymphoproliferation in pooled PC [17, 18]. On the other hand, cytokine production was not totally abrogated at 22 °C with CPD.…”

Section: Discussionmentioning

confidence: 99%

Impact of Storage at 22 °C and Citrate Anticoagulation on the Cytokine Secretion of Mononuclear Leukocytes

1998

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Background and Objectives: Peripheral blood mononuclear cells (PBMC) and cytokines accumulating in platelet concentrates (PC) during storage have been implicated in causing non-hemolytic transfusion reactions, in particular after prolonged PC storage. We investigated the impact of major storage parameters, such as storage time, anticoagulation and temperature, on the capability of PBMC to secrete cytokines. Materials and Methods: In a first study, PBMC from whole blood donations (n = 16) were exposed to standard PC storage conditions: 22 °C, citrate phosphate dextrose (CPD) anticoagulation. Secretion of the cytokines interleukin-1β (IL-1β), IL-2, IL-6 and interferon-γ (IFN-γ) on mitogenic stimulation was measured directly after blood donation and after 1, 3 and 5 days of storage. In a second study, paired whole blood samples (n = 24) were investigated for the mitogenic induction of IL-2, IL-6, IFN-γ and IFN-α. Cytokine values were compared with respect to incubation temperature (22 vs. 37 °C) and anticoagulant (CPD vs. lithium-heparin). Results: PBMC stored at 22 °C with CPD anticoagulation showed an altered cytokine secretion pattern in comparison to freshly donated cells. After storage for 3 days, IL-2 release was decreased (p < 0.05) and after 5 days secretion of all cytokines was significantly decreased, though still detectable (p < 0.01). In the second study, CPD anticoagulation resulted in a significantly impaired cytokine secretion compared with lithium-heparin. Cytokine secretion at 22 °C were significantly lower (p < 0.001) compared with 37 °C for both anticoagulants. Conclusion: Storage of PBMC at 22 °C with CPD leads to an impaired cytokine response, but PBMC are still capable of secreting cytokines after 5 days of storage. PBMC remain a potential source of cytokines even after 5 days of storage in CPD at 22 °C.

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Storage of pooled platelet concentrates

Cited by 49 publications

References 12 publications

Cytokines in Platelet Concentrates Prepared from Pooled Buffy Coats

Cytokines in Platelet Concentrates Prepared from Pooled Buffy Coats

Prevention of Cytokine Accumulation in Platelets Obtained with the COBE Spectra Apheresis System

Impact of Storage at 22 °C and Citrate Anticoagulation on the Cytokine Secretion of Mononuclear Leukocytes

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Storage of pooled platelet concentrates

Cited by 49 publications

References 12 publications

Cytokines in Platelet Concentrates Prepared from Pooled Buffy Coats

Cytokines in Platelet Concentrates Prepared from Pooled Buffy Coats

Prevention of Cytokine Accumulation in Platelets Obtained with the COBE Spectra Apheresis System

Impact of Storage at 22 &deg;C and Citrate Anticoagulation on the Cytokine Secretion of Mononuclear Leukocytes

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Impact of Storage at 22 °C and Citrate Anticoagulation on the Cytokine Secretion of Mononuclear Leukocytes