Storage of platelets in additive solutions: effects of magnesium and/or potassium

Wildt‐Eggen, J. de; Schrijver, J.G.; Bins, M.; Gulliksson, H.

doi:10.1046/j.1537-2995.2002.00012.x

Cited by 74 publications

(74 citation statements)

References 15 publications

(23 reference statements)

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“…[10][11][12] Also, PASs can be formulated to modulate specific PLT characteristics; for example, addition of potassium and magnesium to PAS reduces PLT activation. 13 An observational study showed that the CCI for PLTs stored in plasma had a mean 1-hour CCI of 10.2 6 6.7 versus 10.0 6 6.3 for PLTs stored in a potassium-and magnesium-containing PAS; a solution without these two ingredients had a mean CCI of 8.6 6 6.4 (p < 0.01). 14 Having this in mind, we wondered whether the use of PAS might be able to abrogate the negative effects of PRT on PLT quality, and a four-arm paired study design was developed to allow a good comparison of the various processing, treatment and storage conditions.…”

Section: Resultsmentioning

confidence: 98%

Riboflavin and UV light treatment of platelets: a protective effect of platelet additive solution?

et al. 2015

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Section: Resultsmentioning

confidence: 98%

Riboflavin and UV light treatment of platelets: a protective effect of platelet additive solution?

et al. 2015

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“…Regarding the last limitation, additive solutions have been developed that can attenuate PLT activation. Adding magnesium and potassium ions to the commercial additive solutions PASII and PASIII completely prevented PLT activation, as evidenced by comparable levels of CD62p expression, BMR release, glucose consumption and lactate production (de Wildt-Eggen et al, 2002;Gulliksson et al, 2003;Shanwell et al, 2003). In addition, M-sol, which contains magnesium and potassium ions, reportedly has attenuating effects on PLT activation (Hirayama et al, 2007).…”

Section: Plasma-reduced (Or Concentrated) Plts and Washed Pltsmentioning

confidence: 98%

Current Understanding of Allergic Transfusion Reactions: Incidence, Pathogenesis, Laboratory Tests, Prevention and Treatment

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2013

JJTC

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SummaryNon-haemolytic transfusion reactions are the most common type of transfusion reaction and include transfusion-related acute lung injury, transfusion-associated circulatory overload, allergic reactions, febrile reactions, post-transfusion purpura and graft-versus-host disease. Although life-threatening anaphylaxis occurs rarely, allergic reactions occur most frequently. If possible, even mild transfusion reactions should be avoided because they add to patients' existing suffering. During the last decade, several new discoveries have been made in the field of allergic diseases and transfusion medicine. First, mast cells are not the only cells that are key players in allergic diseases, particularly in the murine immune system. Second, it has been suggested that immunologically active undigested or digested food allergens in a donor's blood may be transferred to a recipient who is allergic to these antigens, causing anaphylaxis. Third, washed platelets have been shown to be effective for preventing allergic transfusion reactions, although substantial numbers of platelets are lost during washing procedures, and platelet recovery after transfusion may not be equivalent to that with unwashed platelets. This review describes allergic transfusion reactions, including the above-mentioned points, and focusses on their incidence, pathogenesis, laboratory tests, prevention and treatment.

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“…Storing platelets in additive solution containing magnesium and potassium (PASIIIM) improves the functionality of the platelets, as measured by glycolysis, pH, morphology, adenosine triphosphate (ATP), and CD62 expression, [12] and may allow a reduction of the amount of plasma required to be carried over to the final unit, facilitating some methods of viral inactivation and making available greater amounts of plasma for other needs. [13] Platelet additive solution replaces 70-80% of the plasma in the original platelet unit; the final medium contains 20-30% donor plasma.…”

Section: Discussionmentioning

confidence: 99%

Extended shelf life of random donor platelets stored for 7 days in platelet additive solution at different temperatures

2014

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P latelets are the second most numerous corpuscles in the blood. They are routinely stored in plasma for 5 days at an average temperature of 22°C. Research in the storage of platelets suggests that there are three fundamental quality standard parameters that must be considered for a proper evaluation of the effects of prolonging the shelf life of platelet concentrates, namely, platelet count (PLT), pH value, and absence of bacteria. Platelets' functions should be better maintained in additive solutions with all the parameters necessary to establish their efficacy. Gulliksson (2000) [2] highlighted some other characteristics in using an additive solution for substitution of plasma as a storage medium for platelet concentrates (PCs). The present study was conducted with an aim to extend the shelf life of random donor platelets stored for 7 days with and without additive solution at temperatures of 22°C, 18°C, and 16°C.Background: Platelets are routinely stored in plasma for 5 days at an average temperature of 22°C. In the present study, the shelf life of random donor platelets was extended by storing for 7 days with and without additive solution at temperatures of 22°C, 18°C, and 16°C. Methods:Random donor platelets were stored in 100% plasma and 20%/80% platelet additive solution. The data were compared using paired "t"-test. The confidence limit was kept at 95%, hence a "p" < 0.05 was considered to be statistically significant. Results:Out of total 150 samples, 148 samples were analyzed and 2 were discarded due to the bacterial contamination on day 7 at 22°C without platelet additive solution. A significant difference in platelet count, platelet factor 3 (PF 3), glucose, lactate dehydrogenase (LDH), and platelet aggregation was observed on day 7 (p < 0.001) at 16°C in without platelet additive solution. In platelet additive solution, the mean values of platelet count, platelet distribution width (PDW), LDH, and pH showed no significant difference on day 7 at 22°C, 18°C, and 16°C. Only significant differences were observed in the levels of mean platelet volume (MPV), PF 3, glucose, and platelet aggregation on day 7 (p < 0.001) at 16°C of the storage period. Conclusion: Random donor platelets functions are better maintained in platelet additive solution as compared to plasma at a lower temperature of 18°C but not at 16°C, on the 7 th day. (Biomed J 2014;37:211-217)

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Storage of platelets in additive solutions: effects of magnesium and/or potassium

Abstract: The combination of Mg(2+) and K(+) gave significantly (p < 0.05) lower platelet CD62 expression in the storage medium than in PAS-2. The effects of these differences on platelet metabolism and in vivo properties remain to be investigated.

Cited by 74 publications

References 15 publications

Riboflavin and UV light treatment of platelets: a protective effect of platelet additive solution?

Riboflavin and UV light treatment of platelets: a protective effect of platelet additive solution?

Current Understanding of Allergic Transfusion Reactions: Incidence, Pathogenesis, Laboratory Tests, Prevention and Treatment

Extended shelf life of random donor platelets stored for 7 days in platelet additive solution at different temperatures

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