Storage of platelets at 4°C in platelet additive solutions prevents aggregate formation and preserves platelet functional responses

Abstract

Storage of AP at 4°C leads to low level GPIIb-IIIa activation and results in aggregate formation over time. Separating the PLTs from the plasma component and storing them in PAS at 4°C resolves aggregate formation and preserves the metabolic and functional responses of these stored PLTs.

Cold storage of platelets in platelet additive solution: an in vitro comparison of two Food and Drug Administration–approved collection and storage systems

“…Although PLTs can be cold‐stored in plasma, an increased risk of PLT clumping during storage due to activation of the glycoprotein (GP) IIb/IIIa receptor and fibrinogen binding can result in a high discard rate . Recent work from our group has shown that cold storage of PLTs in a PLT additive solution (PAS) can minimize cold PLT clumping by dilution of plasma fibrinogen while preserving PLT function for at least 15 days …”

Platelet Additive Solutions: A Review of the Latest Developments and Their Clinical Implications

“…Similarly, PAS could play a role in optimizing conditions for cold-stored platelets [16]. This study showed that at 4 ° C the GpIIb/IIIa fibrinogen receptor is activated by the cold and that the use of PAS resulted in less aggregate formation due to dilution of fibrinogen.…”

Implications of the US Food and Drug Administration draft guidance for mitigating septic reactions from platelet transfusions

“…More recent data showed that alternate storage, such as in platelet additive solution, can lengthen the shelf-life. 23,24 Also the platelets are more hemostatically active, which could be of use in bleeding patients. Although cold-stored platelets are cleared more rapidly, these platelet products may be more suitable for actively bleeding patients, particularly trauma patients.…”