Storage of ADSOL‐Preserved Red Cells at 2.5 and 5.5°C: Comparable Retention of in vitro Properties¹

Abstract

Previous studies with CPDA-1 and Nutricel preserving solutions indicated that red cell properties were affected by small differences in storage temperature. The influence of a 3 degrees C differential on the preservation of the in vitro properties of ADSOL-preserved (AS-1) red blood cells was investigated in a paired study. AS-1 red blood cells were stored at 2.5 and 5.5 degrees C for 42 days. Extent of hemolysis, glucose consumption and pH levels were comparable at the two storage temperatures. Lactate levels were slightly, but significantly higher at 5.5 degrees C. ATP levels were slightly but significantly higher at 2.5 degrees C, only during the later part of the storage period. 2.3-DPG levels were slightly better retained at 2.5 degrees C after 7 days of storage. Holding units of whole blood for either 1 or 8 h at ambient temperature after phlebotomy prior to processing did not influence the types of temperature-dependent changes. The differences as a function of storage temperature were small and appear to be of no practical importance in connection with the storage of AS-1 red blood cells.

Transfusion immunomodulation — the case for leukoreduced and (perhaps) washed transfusions

“…It is well known that WBCs release many potentially harmful proinflammatory and prothrombotic mediators during storage, contributing to what is known as the “storage lesion” (Table I) [7; 8; 9; 10; 11; 12; 13; 14; 15; 16; 17; 18; 19; 20; 21; 22; 23]. Removal of leukocytes before storage partially or completely prevents the accumulation of some of these proinflammatory mediators [24; 25].…”

New frontiers in transfusion biology: identification and significance of mediators of morbidity and mortality in stored red blood cells

“…1). Decreased 2,3‐bisphosphoglygerate (2,3‐BPG), 16 which aids oxygen unloading from hemoglobin (Hb) in tissues, and severe deficiencies of nitric oxide, 17 needed for normal vasodilatation in the microcirculation, have been described along with rheologic and morphologic changes. Oxidative damage resulting from oxygen dissociation from Hb generates superoxide, which generates a hydroxyl radical upon interaction with iron 18 .…”

Does prolonged storage of red blood cells cause harm?

“…Most blood providers extended the RBC shelf life from 21 to 28 days with the addition of phosphate to the solution, to 35 days with the addition of adenine, and to 42 days when additive solutions became widely introduced (Hogman et al , 1978; Beutler & West, 1979; Hogman, 1985; Moroff et al , 1990; Hess, 2006). A brief survey (Table I) documents the variability in the approved versus the practiced RBC shelf life in different healthcare systems (Sparrow, 2012).…”