Storage lipids as a source of carbon skeletons for asparagine synthesis in germinating seeds of yellow lupine (Lupinus luteus L.)

Borek, Sławomir; Ratajczak, Lech

doi:10.1016/j.jplph.2009.12.010

Cited by 18 publications

(34 citation statements)

References 56 publications

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“…Malate in turn, after transport to the cytoplasm, is converted to oxaloacetate. Finally, gluconeogenesis and the synthesis of sugars are the processes which are a form of carbon transport especially in germinating seeds proceed [58,75]. …”

Section: Hydrolysis Of Storage Seed Lipidsmentioning

confidence: 99%

Metabolic Processes During Seed Germination

Ali¹,

Elozeiri²

2017

Advances in Seed Biology

125

102

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Seed germination is crucial stage in plant development and can be considered as a determinant for plant productivity. Physiological and biochemical changes followed by morphological changes during germination are strongly related to seedling survival rate and vegetative growth which consequently affect yield and quality. This study is aimed to focus on proceeding of the most vital metabolic processes namely reserve mobilization, phytohormonal regulation, glyoxylate cycle and respiration process under either stressful or non-stressful conditions that may be led to suggest and conduct the more successful experimental improvements. Seed imbibition triggered the activation of various metabolic processes such as synthesis of hydrolytic enzymes which resulted in hydrolysis of reserve food into simple available form for embryo uptake. Abiotic stresses potentially affect seed germination and seedling establishment through various factors, such as a reduction in water availability, changes in the mobilization of stored reserves, hormonal balance alteration and affecting the structural organization of proteins. Recent strategies for improving seed quality involved classical genetic, molecular biology and invigoration treatments known as priming treatments. H 2 O 2 accumulation and associated oxidative damages together with a decline in antioxidant mechanisms can be regarded as a source of stress that may suppress germination. Seed priming was aimed primarily to control seed hydration by lowering external water potential, or shortening the hydration period.

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Section: Hydrolysis Of Storage Seed Lipidsmentioning

confidence: 99%

Metabolic Processes During Seed Germination

Ali¹,

Elozeiri²

2017

Advances in Seed Biology

125

102

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“…In seedling axes, sucrose (?S) enhanced the carbon flow from lipid into amino acids but in seedling cotyledons, the carbon flow was higher in -S conditions. Contrary to this, in isolated embryo axes and cotyledons cultured in vitro, the carbon flow was more intense in sucrose-fed conditions (?S) (Borek et al 2003;Borek and Ratajczak 2010). One of the key enzymes involved in pathways of carbon flow from lipids into amino acids is cytosolic aconitase ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Simultaneously, mRNA levels for cytosolic aconitase and cytosolic isocitrate dehydrogenase are lower (Borek and Nuc 2011). Higher activity and mRNA level for cytosolic aconitase and cytosolic isocitrate dehydrogenase in yellow lupin organs fed with sucrose are correlated with the enhanced carbon flow from lipid into amino acids, because amino acid synthesis from lipid is significantly enhanced by sucrose in embryo axes and cotyledons (Borek and Ratajczak 2010).…”

Section: Introductionmentioning

confidence: 99%

“…In the reaction catalyzed by phosphoenolpyruvate carboxykinase, oxaloacetate is converted to phosphoenolpyruvate, which starts the gluconeogenesis pathway (Graham 2008). The above-mentioned process is well documented in oil-storing seeds but occurs in germinating lupin seeds as well (Borek et al 2003Borek and Ratajczak 2010). During lupin seed germination, not all storage lipid is converted to sucrose.…”

Section: Introductionmentioning

confidence: 99%

“…1). In this process, cytosolic isocitrate dehydrogenase plays an important role (Borek et al 2003;Borek and Ratajczak 2010). Further research has shown that the intensity of lipid breakdown and carbon flow from lipid to amino acids depends on the lipid content in lupin seeds.…”

Section: Introductionmentioning

confidence: 99%

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Diverse regulation by sucrose of enzymes involved in storage lipid breakdown in germinating lupin seeds

Borek

Kubala

2013

Acta Physiol Plant

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The regulatory function of sucrose in the activity of lipid-degrading enzymes was investigated in germinating seeds of yellow lupin (Lupinus luteus L.), white lupin (Lupinus albus L.) and Andean lupin (Lupinus mutabilis Sweet). The study was conducted on isolated embryo axes, excised cotyledons and seedlings cultured in vitro for 96 h on medium with 60 mM sucrose or without the sugar. The activity of lipase (lipolysis), acylCoA oxidase and catalase (fatty acid b-oxidation) was enhanced in all studied organs cultured on medium without sucrose. The activity of cytosolic aconitase (glyoxylate cycle) was stimulated by sucrose in seedling axes and isolated embryo axes, whereas in seedling cotyledons and excised cotyledons, it was inhibited. The regulatory function of sucrose in phosphoenolpyruvate carboxykinase (gluconeogenesis) was observed only in isolated embryo axes and the activity was lower in carbohydrate deficiency conditions. The peculiar features of storage lipid breakdown in germinating lupin seeds and its regulation by sucrose are discussed.

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