Storage effects on the Cole-Cole parameters of erythrocyte suspensions

Abstract

Chemical alterations of red blood cells (RBCs) during storage eventually affect the electrical properties of blood. In this study, the physiological parameters such as extracellular (SAGM + CPD + residual plasma) Na(+), K(+), Cl(-), pH, 2,3-DPG and ATP together with the Cole-Cole parameters were measured using erythrocyte suspensions from 51 male donors (31 donors form the training set and 20 donors are used for testing), on the 0th, 10th, 21st, 35th and 42nd days of storage. During storage, while the surrounding fluid resistance (R(e)) and the effective cell membrane capacitance (C(m)) increased progressively with time, the intracellular fluid resistance (R(i)) has decreased. Storage of RBCs resulted in a rise in K(+) and a fall in Na(+), Cl(-), pH, 2,3-DPG and ATP. Accordingly, electrical parameters were all correlated with Na(+), K(+), Cl(-), pH and ATP at varying levels. By applying multi-regression analysis, it is concluded that R(i), R(e) and C(m) are appropriate for modeling Na(+), K(+), Cl(-), pH and ATP during storage.

Effects of Physiological Parameters on the Electrical Properties of Blood Bank Stored Erythrocyte Suspensions

“…While Cl -ions enter the cell, bicarbonate ions leave the cell [4], [6]. The cell swells with Cl -and a shape change occurs from discocyte to spheroechinocyte shape, resulting in an increased form factor [9]. The extracellular resistance is inversely proportional to this form factor.…”

“…Physiological and electrical measurements of erythrocyte suspensions were performed at room temperature [9].…”

Comparison of Stored Umbilical Cord Blood and Adult Donor Blood: Transfusion Feasibility

“…Numerous studies reported that K + , 2,3-BPG, ATP, and pH values in stored blood and blood components change with time, and research has been conducted in an effort to increase the quality of stored blood [24,25,26,27,28,29,30]. Studies on the biochemical and hematological properties of stored UCB, however, are less common,[26,27,28,29,30].…”

“…Studies on the biochemical and hematological properties of stored UCB, however, are less common,[26,27,28,29,30]. Studies on the biochemical and hematological properties of stored UCB, however, are less common.…”

Physiological quality assessment of stored whole blood by means of electrical measurements

“…3), the extracellular Na + , K + and Cl -concentrations, 2,3-DPG, ATP and pH were measured at room temperature as described in the earlier study [36].…”