Storage conditions, sample integrity, interferences, and a decision tool for investigating unusual high-sensitivity cardiac troponin results

Successful management of ventricular bigeminy using lidocaine in a horse anaesthetised for emergency laparotomy

“…The serum was thawed 42 days later and quickly sent for analysis, ensuring minimal interference with cTnI measurement. 15 The determined serum cTnI concentration was 0.004 ng/mL, falling within the normal range. This value aligned with preoperative cTnI levels observed in horses that successfully underwent colic surgery.…”

“…Blood was collected in a clot activator tube, promptly centrifuged to extract serum, and stored at 4°C before being frozen at −80°C within 24 h after sampling. The serum was thawed 42 days later and quickly sent for analysis, ensuring minimal interference with cTnI measurement 15 . The determined serum cTnI concentration was 0.004 ng/mL, falling within the normal range.…”

Incidence and Etiology of Poor Duplicate Ortho High-Sensitivity Cardiac Troponin I Results in a Community Hospital Emergency Department

“…Briefly, duplicate testing (laboratory initiative) for the Ortho hs-cTnI assay was performed with the average result reported if the 2 results met the prespecified criteria for agreement ( Supplemental Table S1 ). 3 , 4 Samples yielding poor repeat measurements were not reported for the Ortho hs-cTnI assay, with the sample sent immediately for testing with the Abbott hs-cTnI assay. 2 , 3 Data were obtained from January 14, 2021 to February 8, 2022 from the laboratory information system on samples from ED patients that were not reported with the Ortho hs-cTnI assay due to poor reproducibility.…”

High-Sensitivity Cardiac Troponin Publications during the COVID-19 Pandemic (2020–2022)

“…The role of an objective biomarker here, such as hs-cTn, is extremely valuable; albeit not all hs-cTn elevations following COVID-19 infection or immunization against the virus reflect ongoing, acute injury [ 7 ]. Macrocomplexes may be a possible culprit for some of these elevations in some patients in this setting, and outside of COVID-19, macrocomplexes and other new interferences as well as different combinations of known (pre-analytical) variables may result in discordant hs-cTn levels as highlighted in articles published in the past couple of years [ 7 , 8 , 9 , 10 , 11 , 12 ].…”